Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates
Polymeric nanoparticles (NPs) present some ideal properties as biomedical nanocarriers for targeted drug delivery such as enhanced translocation through body barriers. Biopolymers, such as polyhydroxyalkanoates (PHAs) are gaining attention as nanocarrier biomaterials due to their inherent biocompati...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220336/full |
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| author | Francisco G. Blanco Francisco G. Blanco Roberto Vázquez Ana M. Hernández-Arriaga Ana M. Hernández-Arriaga Pedro García M. Auxiliadora Prieto M. Auxiliadora Prieto |
| author_facet | Francisco G. Blanco Francisco G. Blanco Roberto Vázquez Ana M. Hernández-Arriaga Ana M. Hernández-Arriaga Pedro García M. Auxiliadora Prieto M. Auxiliadora Prieto |
| author_sort | Francisco G. Blanco |
| collection | DOAJ |
| description | Polymeric nanoparticles (NPs) present some ideal properties as biomedical nanocarriers for targeted drug delivery such as enhanced translocation through body barriers. Biopolymers, such as polyhydroxyalkanoates (PHAs) are gaining attention as nanocarrier biomaterials due to their inherent biocompatibility, biodegradability, and ability to be vehiculized through hydrophobic media, such as the lung surfactant (LS). Upon colonization of the lung alveoli, below the LS layer, Streptococcus pneumoniae, causes community-acquired pneumonia, a severe respiratory condition. In this work, we convert PHA NPs into an antimicrobial material by the immobilization of an enzybiotic, an antimicrobial enzyme, via a minimal PHA affinity tag. We first produced the fusion protein M711, comprising the minimized PHA affinity tag, MinP, and the enzybiotic Cpl-711, which specifically targets S. pneumoniae. Then, a PHA nanoparticulate suspension with adequate physicochemical properties for pulmonary delivery was formulated, and NPs were decorated with M711. Finally, we assessed the antipneumococcal activity of the nanosystem against planktonic and biofilm forms of S. pneumoniae. The resulting system displayed sustained antimicrobial activity against both, free and sessile cells, confirming that tag-mediated immobilization of enzybiotics on PHAs is a promising platform for bioactive antimicrobial functionalization. |
| first_indexed | 2024-03-13T02:52:09Z |
| format | Article |
| id | doaj.art-0d748cecd53342299ec34a74a3229b0e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T02:52:09Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-0d748cecd53342299ec34a74a3229b0e2023-06-28T09:04:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-06-011110.3389/fbioe.2023.12203361220336Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoatesFrancisco G. Blanco0Francisco G. Blanco1Roberto Vázquez2Ana M. Hernández-Arriaga3Ana M. Hernández-Arriaga4Pedro García5M. Auxiliadora Prieto6M. Auxiliadora Prieto7Polymer Biotechnology Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB–CSIC), Madrid, SpainInterdisciplinary Platform of Sustainable Plastics towards a Circular Economy, Spanish National Research Council (SusPlast-CSIC), Madrid, SpainProtein Engineering Against Antibiotic Resistance Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB-CSIC), Madrid, SpainPolymer Biotechnology Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB–CSIC), Madrid, SpainInterdisciplinary Platform of Sustainable Plastics towards a Circular Economy, Spanish National Research Council (SusPlast-CSIC), Madrid, SpainProtein Engineering Against Antibiotic Resistance Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB-CSIC), Madrid, SpainPolymer Biotechnology Group, Microbial and Plant Biotechnology Department, Margarita Salas Center for Biological Research (CIB–CSIC), Madrid, SpainInterdisciplinary Platform of Sustainable Plastics towards a Circular Economy, Spanish National Research Council (SusPlast-CSIC), Madrid, SpainPolymeric nanoparticles (NPs) present some ideal properties as biomedical nanocarriers for targeted drug delivery such as enhanced translocation through body barriers. Biopolymers, such as polyhydroxyalkanoates (PHAs) are gaining attention as nanocarrier biomaterials due to their inherent biocompatibility, biodegradability, and ability to be vehiculized through hydrophobic media, such as the lung surfactant (LS). Upon colonization of the lung alveoli, below the LS layer, Streptococcus pneumoniae, causes community-acquired pneumonia, a severe respiratory condition. In this work, we convert PHA NPs into an antimicrobial material by the immobilization of an enzybiotic, an antimicrobial enzyme, via a minimal PHA affinity tag. We first produced the fusion protein M711, comprising the minimized PHA affinity tag, MinP, and the enzybiotic Cpl-711, which specifically targets S. pneumoniae. Then, a PHA nanoparticulate suspension with adequate physicochemical properties for pulmonary delivery was formulated, and NPs were decorated with M711. Finally, we assessed the antipneumococcal activity of the nanosystem against planktonic and biofilm forms of S. pneumoniae. The resulting system displayed sustained antimicrobial activity against both, free and sessile cells, confirming that tag-mediated immobilization of enzybiotics on PHAs is a promising platform for bioactive antimicrobial functionalization.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220336/fullpolyhydroxyalkanoatesantimicrobial nanoparticlesantimicrobial materialsenzybioticsdrug delivery |
| spellingShingle | Francisco G. Blanco Francisco G. Blanco Roberto Vázquez Ana M. Hernández-Arriaga Ana M. Hernández-Arriaga Pedro García M. Auxiliadora Prieto M. Auxiliadora Prieto Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates Frontiers in Bioengineering and Biotechnology polyhydroxyalkanoates antimicrobial nanoparticles antimicrobial materials enzybiotics drug delivery |
| title | Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates |
| title_full | Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates |
| title_fullStr | Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates |
| title_full_unstemmed | Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates |
| title_short | Enzybiotic-mediated antimicrobial functionalization of polyhydroxyalkanoates |
| title_sort | enzybiotic mediated antimicrobial functionalization of polyhydroxyalkanoates |
| topic | polyhydroxyalkanoates antimicrobial nanoparticles antimicrobial materials enzybiotics drug delivery |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1220336/full |
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