NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease
Mesenchymal stem cells (MSCs) are becoming more popular in therapy. Therefore, in-depth studies on mesenchymal stem cells in therapy are urgently needed. However, the difficulty in culturing and propagating MSCs in vitro complicates potential studies on MSCs in a murine model. OP9 cells are a stroma...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023052465 |
| _version_ | 1827892248979701760 |
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| author | Yutong Chen Weicheng Meng Guangming Ren Ning An Jing Zhang Zhixin Liu Xiaoshuang Wu Wen Yin Xingbin Hu Zheng Liu Fan Feng Yaozhen Chen |
| author_facet | Yutong Chen Weicheng Meng Guangming Ren Ning An Jing Zhang Zhixin Liu Xiaoshuang Wu Wen Yin Xingbin Hu Zheng Liu Fan Feng Yaozhen Chen |
| author_sort | Yutong Chen |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are becoming more popular in therapy. Therefore, in-depth studies on mesenchymal stem cells in therapy are urgently needed. However, the difficulty in culturing and propagating MSCs in vitro complicates potential studies on MSCs in a murine model. OP9 cells are a stromal cell line from mouse bone marrow, which have similar characteristics and functions to MSCs and can maintain their original characteristics. Because of these properties, OP9 cells have become a suitable substitute for research on MSCs. Previously, we have found that MSCs can cure inflammatory bowel disease in mice. In this study, we aimed to investigate whether OP9 cells can functionally regulate and alleviate inflammatory diseases. We evaluated the therapeutic effect of OP9 cells in the mouse model of inflammatory bowel disease and found OP9 cells were able to ameliorate inflammatory bowel disease. We explored the existence of NLRP3 inflammasome in OP9 cells, and showed better therapeutic effects when the NLRP3 inflammasome was suppressed. Thus, OP9 cell line is similar to MSCs in characteristic and function, and is an ideal substitute for MSCs research. The preliminary exploration of the inflammasome system in OP9 cells lays a theoretical and methodological foundation for further study of MSCs. |
| first_indexed | 2024-03-12T21:37:08Z |
| format | Article |
| id | doaj.art-0d74c2532dec4e55a4495f19273dfbdf |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:37:08Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-0d74c2532dec4e55a4495f19273dfbdf2023-07-27T05:58:20ZengElsevierHeliyon2405-84402023-07-0197e18038NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel diseaseYutong Chen0Weicheng Meng1Guangming Ren2Ning An3Jing Zhang4Zhixin Liu5Xiaoshuang Wu6Wen Yin7Xingbin Hu8Zheng Liu9Fan Feng10Yaozhen Chen11Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDivision of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaDepartment of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, ChinaKobilka Institute of Innovative Drug Discovery, School of Medicine, Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, ChinaDivision of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, 710032, China; Corresponding author.Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China; Corresponding author.Mesenchymal stem cells (MSCs) are becoming more popular in therapy. Therefore, in-depth studies on mesenchymal stem cells in therapy are urgently needed. However, the difficulty in culturing and propagating MSCs in vitro complicates potential studies on MSCs in a murine model. OP9 cells are a stromal cell line from mouse bone marrow, which have similar characteristics and functions to MSCs and can maintain their original characteristics. Because of these properties, OP9 cells have become a suitable substitute for research on MSCs. Previously, we have found that MSCs can cure inflammatory bowel disease in mice. In this study, we aimed to investigate whether OP9 cells can functionally regulate and alleviate inflammatory diseases. We evaluated the therapeutic effect of OP9 cells in the mouse model of inflammatory bowel disease and found OP9 cells were able to ameliorate inflammatory bowel disease. We explored the existence of NLRP3 inflammasome in OP9 cells, and showed better therapeutic effects when the NLRP3 inflammasome was suppressed. Thus, OP9 cell line is similar to MSCs in characteristic and function, and is an ideal substitute for MSCs research. The preliminary exploration of the inflammasome system in OP9 cells lays a theoretical and methodological foundation for further study of MSCs.http://www.sciencedirect.com/science/article/pii/S2405844023052465OP9 cellNLRP3 inflammasomeIL-1βPyroptosisInflammatory bowel disease |
| spellingShingle | Yutong Chen Weicheng Meng Guangming Ren Ning An Jing Zhang Zhixin Liu Xiaoshuang Wu Wen Yin Xingbin Hu Zheng Liu Fan Feng Yaozhen Chen NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease Heliyon OP9 cell NLRP3 inflammasome IL-1β Pyroptosis Inflammatory bowel disease |
| title | NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease |
| title_full | NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease |
| title_fullStr | NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease |
| title_full_unstemmed | NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease |
| title_short | NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease |
| title_sort | nlrp3 inflammasome inhibition of op9 cells enhance therapy for inflammatory bowel disease |
| topic | OP9 cell NLRP3 inflammasome IL-1β Pyroptosis Inflammatory bowel disease |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023052465 |
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