Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat
Grain protein content (GPC) and yield are of two important traits in wheat, but their negative correlation has hampered their simultaneous improvement in conventional breeding. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is an important genetic resource for wheat quality improvement. In th...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00464/full |
| _version_ | 1818206319695364096 |
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| author | Jia Liu Jia Liu Lin Huang Lin Huang Changquan Wang Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zhenzhen Wang Lan Xiang Xiaoying Zhong Fangyi Gong Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu |
| author_facet | Jia Liu Jia Liu Lin Huang Lin Huang Changquan Wang Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zhenzhen Wang Lan Xiang Xiaoying Zhong Fangyi Gong Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu |
| author_sort | Jia Liu |
| collection | DOAJ |
| description | Grain protein content (GPC) and yield are of two important traits in wheat, but their negative correlation has hampered their simultaneous improvement in conventional breeding. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is an important genetic resource for wheat quality improvement. In this study, we report a genome-wide association study (GWAS) using 13116 DArT-seq markers to characterize GPC in 161 wheat lines derived from wild emmer. Using a general linear model, we identified 141 markers that were significantly associated with GPC, and grouped into 48 QTL regions. Using both general linear model and mixed linear model, we identified four significant markers that were grouped into two novel QTL regions on chromosomes 2BS (QGpc.cd1-2B.1) and 7BL (QGpc.cd1-7B.2). The two QTLs have no negative effects on thousand kernel weight (TKW) and should be useful for simultaneous improvement of GPC and TKW in wheat breeding. Searches of public databases revealed 61 putative candidate/flanking genes related to GPC. The putative proteins of interest were grouped in four main categories: enzymes, kinase proteins, metal transport-related proteins, and disease resistance proteins. The linked markers and associated candidate genes provide essential information for cloning genes related to high GPC and performing marker-assisted breeding in wheat. |
| first_indexed | 2024-12-12T04:11:09Z |
| format | Article |
| id | doaj.art-0d77856635ca42ba869f32545f408d79 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T04:11:09Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0d77856635ca42ba869f32545f408d792022-12-22T00:38:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00464437520Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer WheatJia Liu0Jia Liu1Lin Huang2Lin Huang3Changquan Wang4Yaxi Liu5Yaxi Liu6Zehong Yan7Zehong Yan8Zhenzhen Wang9Lan Xiang10Xiaoying Zhong11Fangyi Gong12Youliang Zheng13Youliang Zheng14Dengcai Liu15Dengcai Liu16Bihua Wu17Bihua Wu18Triticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaGrain protein content (GPC) and yield are of two important traits in wheat, but their negative correlation has hampered their simultaneous improvement in conventional breeding. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is an important genetic resource for wheat quality improvement. In this study, we report a genome-wide association study (GWAS) using 13116 DArT-seq markers to characterize GPC in 161 wheat lines derived from wild emmer. Using a general linear model, we identified 141 markers that were significantly associated with GPC, and grouped into 48 QTL regions. Using both general linear model and mixed linear model, we identified four significant markers that were grouped into two novel QTL regions on chromosomes 2BS (QGpc.cd1-2B.1) and 7BL (QGpc.cd1-7B.2). The two QTLs have no negative effects on thousand kernel weight (TKW) and should be useful for simultaneous improvement of GPC and TKW in wheat breeding. Searches of public databases revealed 61 putative candidate/flanking genes related to GPC. The putative proteins of interest were grouped in four main categories: enzymes, kinase proteins, metal transport-related proteins, and disease resistance proteins. The linked markers and associated candidate genes provide essential information for cloning genes related to high GPC and performing marker-assisted breeding in wheat.https://www.frontiersin.org/article/10.3389/fpls.2019.00464/fullwild emmer wheatcommon wheatGPCwide hybridizationGWAS |
| spellingShingle | Jia Liu Jia Liu Lin Huang Lin Huang Changquan Wang Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zhenzhen Wang Lan Xiang Xiaoying Zhong Fangyi Gong Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat Frontiers in Plant Science wild emmer wheat common wheat GPC wide hybridization GWAS |
| title | Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat |
| title_full | Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat |
| title_fullStr | Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat |
| title_full_unstemmed | Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat |
| title_short | Genome-Wide Association Study Reveals Novel Genomic Regions Associated With High Grain Protein Content in Wheat Lines Derived From Wild Emmer Wheat |
| title_sort | genome wide association study reveals novel genomic regions associated with high grain protein content in wheat lines derived from wild emmer wheat |
| topic | wild emmer wheat common wheat GPC wide hybridization GWAS |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00464/full |
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