Morphofunctional changes in the rat's liver of different ages after L-methionine administration
Background: Literature data on the effect of methionine on functional activity and, especially, on morphological changes in the liver parenchyma in animals of different ages are sporadic, and research results are often ambiguous. Aim: The purpose of this work was to study and compare the morphof...
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| Format: | Article |
| Language: | English |
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Kazimierz Wielki University
2022-01-01
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| Series: | Journal of Education, Health and Sport |
| Subjects: | |
| Online Access: | https://apcz.umk.pl/JEHS/article/view/37162 |
| _version_ | 1818249208717639680 |
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| author | Roman Yanko Elena Chaka Mikhail Levashov |
| author_facet | Roman Yanko Elena Chaka Mikhail Levashov |
| author_sort | Roman Yanko |
| collection | DOAJ |
| description |
Background: Literature data on the effect of methionine on functional activity and, especially, on morphological changes in the liver parenchyma in animals of different ages are sporadic, and research results are often ambiguous.
Aim: The purpose of this work was to study and compare the morphofunctional changes in the liver of rats of different ages on prolonged administration of L-methionine.
Material and Methods: The experiment was performed on 48 male Wistar rats of 3 and 15 months of age. Animals of the experimental group received L-methionine at a dose of 250 mg/kg body weight in addition to the standard diet, daily for 21 days. Histological preparations were prepared from liver tissue by a standard technique. Morphometry was performed on digital images using the computer program «Image J». Succinate dehydrogenase activity and protein concentration were determined in the suspension of hepatocyte mitochondria.
Results: It was revealed that 21-day administration of L-methionine to rats led to hypertrophy of the hepatocyte nucleus, an increase in the nuclear-cytoplasmic ratio, the number of binuclear hepatocytes, and the nucleolus in the cell nucleus. The relative area of the sinusoids network increased by 50% in 3-month-old animals. This indicated a better blood filling of the liver parenchyma. The increase in succinate dehydrogenase activity and protein concentration was revealed in the suspension of hepatocyte mitochondria of the experimental rats. This indicated an increase in the mitochondria energy potential and protein-synthetic activity.
Conclusions: The administration of prophylactic doses of methionine to healthy rats leads to the appearance of pronounced morphological and functional signs of increased activity of hepatocytes. The severity of this effect has a distinct age-dependent character. In young rats, it is more pronounced than in mature rats. The results of the study are important for practical medicine when using methionine for therapeutic and prophylactic purposes.
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| first_indexed | 2024-12-12T15:32:51Z |
| format | Article |
| id | doaj.art-0d784b7a32c14b5bb135721f88f9e423 |
| institution | Directory Open Access Journal |
| issn | 2391-8306 |
| language | English |
| last_indexed | 2024-12-12T15:32:51Z |
| publishDate | 2022-01-01 |
| publisher | Kazimierz Wielki University |
| record_format | Article |
| series | Journal of Education, Health and Sport |
| spelling | doaj.art-0d784b7a32c14b5bb135721f88f9e4232022-12-22T00:20:04ZengKazimierz Wielki UniversityJournal of Education, Health and Sport2391-83062022-01-0112110.12775/JEHS.2022.12.01.010Morphofunctional changes in the rat's liver of different ages after L-methionine administrationRoman Yanko0Elena Chaka1Mikhail Levashov2Bogomoletz Institute of PhysiologyBogomoletz Institute of PhysiologyBogomoletz Institute of Physiology Background: Literature data on the effect of methionine on functional activity and, especially, on morphological changes in the liver parenchyma in animals of different ages are sporadic, and research results are often ambiguous. Aim: The purpose of this work was to study and compare the morphofunctional changes in the liver of rats of different ages on prolonged administration of L-methionine. Material and Methods: The experiment was performed on 48 male Wistar rats of 3 and 15 months of age. Animals of the experimental group received L-methionine at a dose of 250 mg/kg body weight in addition to the standard diet, daily for 21 days. Histological preparations were prepared from liver tissue by a standard technique. Morphometry was performed on digital images using the computer program «Image J». Succinate dehydrogenase activity and protein concentration were determined in the suspension of hepatocyte mitochondria. Results: It was revealed that 21-day administration of L-methionine to rats led to hypertrophy of the hepatocyte nucleus, an increase in the nuclear-cytoplasmic ratio, the number of binuclear hepatocytes, and the nucleolus in the cell nucleus. The relative area of the sinusoids network increased by 50% in 3-month-old animals. This indicated a better blood filling of the liver parenchyma. The increase in succinate dehydrogenase activity and protein concentration was revealed in the suspension of hepatocyte mitochondria of the experimental rats. This indicated an increase in the mitochondria energy potential and protein-synthetic activity. Conclusions: The administration of prophylactic doses of methionine to healthy rats leads to the appearance of pronounced morphological and functional signs of increased activity of hepatocytes. The severity of this effect has a distinct age-dependent character. In young rats, it is more pronounced than in mature rats. The results of the study are important for practical medicine when using methionine for therapeutic and prophylactic purposes. https://apcz.umk.pl/JEHS/article/view/37162methionineliver |
| spellingShingle | Roman Yanko Elena Chaka Mikhail Levashov Morphofunctional changes in the rat's liver of different ages after L-methionine administration Journal of Education, Health and Sport methionine liver |
| title | Morphofunctional changes in the rat's liver of different ages after L-methionine administration |
| title_full | Morphofunctional changes in the rat's liver of different ages after L-methionine administration |
| title_fullStr | Morphofunctional changes in the rat's liver of different ages after L-methionine administration |
| title_full_unstemmed | Morphofunctional changes in the rat's liver of different ages after L-methionine administration |
| title_short | Morphofunctional changes in the rat's liver of different ages after L-methionine administration |
| title_sort | morphofunctional changes in the rat s liver of different ages after l methionine administration |
| topic | methionine liver |
| url | https://apcz.umk.pl/JEHS/article/view/37162 |
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