Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling
Ex-vivo molecular profiling has recently emerged as a promising method for intraoperative tissue identification, especially in neurosurgery. The short-term storage of resected samples at room temperature is proposed to have negligible influence on the lipid molecular profiles. However, a detailed in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/8/2587 |
| _version_ | 1797444550553239552 |
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| author | Stanislav I. Pekov Evgeny S. Zhvansky Vasily A. Eliferov Anatoly A. Sorokin Daniil G. Ivanov Eugene N. Nikolaev Igor A. Popov |
| author_facet | Stanislav I. Pekov Evgeny S. Zhvansky Vasily A. Eliferov Anatoly A. Sorokin Daniil G. Ivanov Eugene N. Nikolaev Igor A. Popov |
| author_sort | Stanislav I. Pekov |
| collection | DOAJ |
| description | Ex-vivo molecular profiling has recently emerged as a promising method for intraoperative tissue identification, especially in neurosurgery. The short-term storage of resected samples at room temperature is proposed to have negligible influence on the lipid molecular profiles. However, a detailed investigation of short-term molecular profile stability is required to implement molecular profiling in a clinic. This study evaluates the effect of storage media, temperature, and washing solution to determine conditions that provide stable and reproducible molecular profiles, with the help of ambient ionization mass spectrometry using rat cerebral cortex as model brain tissue samples. Utilizing normal saline for sample storage and washing media shows a positive effect on the reproducibility of the spectra; however, the refrigeration shows a negligible effect on the spectral similarity. Thus, it was demonstrated that up to hour-long storage in normal saline, even at room temperature, ensures the acquisition of representative molecular profiles using ambient ionization mass spectrometry. |
| first_indexed | 2024-03-09T13:13:44Z |
| format | Article |
| id | doaj.art-0d80fe3a5f4040ea9f06efe7015d9285 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:13:44Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0d80fe3a5f4040ea9f06efe7015d92852023-11-30T21:38:40ZengMDPI AGMolecules1420-30492022-04-01278258710.3390/molecules27082587Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid ProfilingStanislav I. Pekov0Evgeny S. Zhvansky1Vasily A. Eliferov2Anatoly A. Sorokin3Daniil G. Ivanov4Eugene N. Nikolaev5Igor A. Popov6Skolkovo Institute of Science and Technology, 121205 Moscow, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaSkolkovo Institute of Science and Technology, 121205 Moscow, RussiaMoscow Institute of Physics and Technology, 141701 Dolgoprudny, RussiaEx-vivo molecular profiling has recently emerged as a promising method for intraoperative tissue identification, especially in neurosurgery. The short-term storage of resected samples at room temperature is proposed to have negligible influence on the lipid molecular profiles. However, a detailed investigation of short-term molecular profile stability is required to implement molecular profiling in a clinic. This study evaluates the effect of storage media, temperature, and washing solution to determine conditions that provide stable and reproducible molecular profiles, with the help of ambient ionization mass spectrometry using rat cerebral cortex as model brain tissue samples. Utilizing normal saline for sample storage and washing media shows a positive effect on the reproducibility of the spectra; however, the refrigeration shows a negligible effect on the spectral similarity. Thus, it was demonstrated that up to hour-long storage in normal saline, even at room temperature, ensures the acquisition of representative molecular profiles using ambient ionization mass spectrometry.https://www.mdpi.com/1420-3049/27/8/2587molecular profilingneurosurgeryambient ionization mass spectrometrycosine similarity |
| spellingShingle | Stanislav I. Pekov Evgeny S. Zhvansky Vasily A. Eliferov Anatoly A. Sorokin Daniil G. Ivanov Eugene N. Nikolaev Igor A. Popov Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling Molecules molecular profiling neurosurgery ambient ionization mass spectrometry cosine similarity |
| title | Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling |
| title_full | Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling |
| title_fullStr | Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling |
| title_full_unstemmed | Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling |
| title_short | Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling |
| title_sort | determination of brain tissue samples storage conditions for reproducible intraoperative lipid profiling |
| topic | molecular profiling neurosurgery ambient ionization mass spectrometry cosine similarity |
| url | https://www.mdpi.com/1420-3049/27/8/2587 |
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