In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate
Abstract Background The antifungal efficacy of cold atmospheric microwave plasma (CAMP) against Malassezia pachydermatis has not been to be evaluated. Objective To examine the antifungal effects of CAMP against M. pachydermatis and its synergistic effects with chlorhexidine gluconate (CHX). Methods...
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| Format: | Article |
| Language: | English |
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Wiley
2022-03-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.719 |
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| author | Tae‐Hyun Lee Jae‐Eun Hyun Yeong‐Hun Kang Seung‐Joon Baek Cheol‐Yong Hwang |
| author_facet | Tae‐Hyun Lee Jae‐Eun Hyun Yeong‐Hun Kang Seung‐Joon Baek Cheol‐Yong Hwang |
| author_sort | Tae‐Hyun Lee |
| collection | DOAJ |
| description | Abstract Background The antifungal efficacy of cold atmospheric microwave plasma (CAMP) against Malassezia pachydermatis has not been to be evaluated. Objective To examine the antifungal effects of CAMP against M. pachydermatis and its synergistic effects with chlorhexidine gluconate (CHX). Methods A M. pachydermatis isolate was collected from a dog with otitis externa and Malassezia dermatitis at the Seoul National University Veterinary Medical Teaching Hospital. The antifungal effect was determined by applying CAMP to a M. pachydermatis isolate that was incubated for 3 days at 37°C. After 1, 2, 3 and 5 min of application, the efficacy of the plasma treatment was determined according to the number of colony forming units (CFUs). A mixture consisting of inoculum and CHX was applied to evaluate the synergistic effect of the plasma treatment in the same way. Results The application of CAMP showed significant antifungal effects against M. pachydermatis. The antifungal effect of CAMP was enhanced by an increased exposure time and output power. The application of CAMP with 0.02% and 0.2% CHX resulted in lower survival rates against M. pachydermatis when compared with its sole application at 1 or 2 min. Conclusions The study findings demonstrate that CAMP has a potential as a new antifungal option for M. pachydermatis and has synergistic antifungal effects with CHX in vitro. Clinical applications for CAMP are necessary to assess the antifungal efficacy for patients. |
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| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-12T22:24:44Z |
| publishDate | 2022-03-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj.art-0d81aff8ecc44cadb3999876a25cd9642022-12-22T03:14:12ZengWileyVeterinary Medicine and Science2053-10952022-03-018252452910.1002/vms3.719In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconateTae‐Hyun Lee0Jae‐Eun Hyun1Yeong‐Hun Kang2Seung‐Joon Baek3Cheol‐Yong Hwang4Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul Republic of KoreaInstitute of Animal Medicine College of Veterinary Medicine Gyeongsang National University Jinju Republic of KoreaLaboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul Republic of KoreaLaboratory of Signal Transduction College of Veterinary Medicine Seoul National University Seoul Republic of KoreaLaboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul Republic of KoreaAbstract Background The antifungal efficacy of cold atmospheric microwave plasma (CAMP) against Malassezia pachydermatis has not been to be evaluated. Objective To examine the antifungal effects of CAMP against M. pachydermatis and its synergistic effects with chlorhexidine gluconate (CHX). Methods A M. pachydermatis isolate was collected from a dog with otitis externa and Malassezia dermatitis at the Seoul National University Veterinary Medical Teaching Hospital. The antifungal effect was determined by applying CAMP to a M. pachydermatis isolate that was incubated for 3 days at 37°C. After 1, 2, 3 and 5 min of application, the efficacy of the plasma treatment was determined according to the number of colony forming units (CFUs). A mixture consisting of inoculum and CHX was applied to evaluate the synergistic effect of the plasma treatment in the same way. Results The application of CAMP showed significant antifungal effects against M. pachydermatis. The antifungal effect of CAMP was enhanced by an increased exposure time and output power. The application of CAMP with 0.02% and 0.2% CHX resulted in lower survival rates against M. pachydermatis when compared with its sole application at 1 or 2 min. Conclusions The study findings demonstrate that CAMP has a potential as a new antifungal option for M. pachydermatis and has synergistic antifungal effects with CHX in vitro. Clinical applications for CAMP are necessary to assess the antifungal efficacy for patients.https://doi.org/10.1002/vms3.719antifungal effectschlorhexidine gluconatecold atmospheric microwave plasmaMalassezia pachydermatissynergism |
| spellingShingle | Tae‐Hyun Lee Jae‐Eun Hyun Yeong‐Hun Kang Seung‐Joon Baek Cheol‐Yong Hwang In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate Veterinary Medicine and Science antifungal effects chlorhexidine gluconate cold atmospheric microwave plasma Malassezia pachydermatis synergism |
| title | In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate |
| title_full | In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate |
| title_fullStr | In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate |
| title_full_unstemmed | In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate |
| title_short | In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate |
| title_sort | in vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against malassezia pachydermatis when combined with chlorhexidine gluconate |
| topic | antifungal effects chlorhexidine gluconate cold atmospheric microwave plasma Malassezia pachydermatis synergism |
| url | https://doi.org/10.1002/vms3.719 |
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