Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>)
Silver sillago (<i>Sillago sihama</i>) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of <i>S. sihama</i>. The fish were divided into four groups, such as 1 h of hypoxia...
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MDPI AG
2020-04-01
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| author | Wanida Saetan Changxu Tian Jiawang Yu Xinghua Lin Feixiang He Yang Huang Hongjuan Shi Yulei Zhang Guangli Li |
| author_facet | Wanida Saetan Changxu Tian Jiawang Yu Xinghua Lin Feixiang He Yang Huang Hongjuan Shi Yulei Zhang Guangli Li |
| author_sort | Wanida Saetan |
| collection | DOAJ |
| description | Silver sillago (<i>Sillago sihama</i>) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of <i>S. sihama</i>. The fish were divided into four groups, such as 1 h of hypoxia (hypoxia1h, DO = 1.5 ± 0.1 mg/L), 4 h of hypoxia (hypoxia4h, DO = 1.5 ± 0.1 mg/L), 4 h of reoxygen (reoxygen4h, DO = 8.0 ± 0.2 mg/L) after 4 h of hypoxia (DO = 1.5 mg/L), and normoxia or control (DO = 8.0 ± 0.2 mg/L) groups. Compared to the normoxia group, a total of 3550 genes were identified as differentially expressed genes (DEGs) (log<sub>2</sub>foldchange > 1 and padj < 0.05), including 1103, 1451 and 996 genes in hypoxia1h, hypoxia4h and reoxygen4h groups, respectively. Only 247 DEGs were differentially co-expressed in all treatment groups. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEGs were significantly enriched in steroid biosynthesis, biosynthesis of amino acids, glutathione metabolism and metabolism of xenobiotics by cytochrome P450, ferroptosis and drug metabolism—cytochrome P450 pathways. Of these, the cytochrome P450 (CYP) and glutathione S-transferase (GST) gene families were widely expressed. Our study represents the insights into the underlying molecular mechanisms of hypoxia stress. |
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| last_indexed | 2024-03-10T20:38:25Z |
| publishDate | 2020-04-01 |
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| series | Animals |
| spelling | doaj.art-0d81ddcee51744f09f31566085c8a4d32023-11-19T20:50:17ZengMDPI AGAnimals2076-26152020-04-0110462810.3390/ani10040628Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>)Wanida Saetan0Changxu Tian1Jiawang Yu2Xinghua Lin3Feixiang He4Yang Huang5Hongjuan Shi6Yulei Zhang7Guangli Li8Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaSilver sillago (<i>Sillago sihama</i>) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of <i>S. sihama</i>. The fish were divided into four groups, such as 1 h of hypoxia (hypoxia1h, DO = 1.5 ± 0.1 mg/L), 4 h of hypoxia (hypoxia4h, DO = 1.5 ± 0.1 mg/L), 4 h of reoxygen (reoxygen4h, DO = 8.0 ± 0.2 mg/L) after 4 h of hypoxia (DO = 1.5 mg/L), and normoxia or control (DO = 8.0 ± 0.2 mg/L) groups. Compared to the normoxia group, a total of 3550 genes were identified as differentially expressed genes (DEGs) (log<sub>2</sub>foldchange > 1 and padj < 0.05), including 1103, 1451 and 996 genes in hypoxia1h, hypoxia4h and reoxygen4h groups, respectively. Only 247 DEGs were differentially co-expressed in all treatment groups. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEGs were significantly enriched in steroid biosynthesis, biosynthesis of amino acids, glutathione metabolism and metabolism of xenobiotics by cytochrome P450, ferroptosis and drug metabolism—cytochrome P450 pathways. Of these, the cytochrome P450 (CYP) and glutathione S-transferase (GST) gene families were widely expressed. Our study represents the insights into the underlying molecular mechanisms of hypoxia stress.https://www.mdpi.com/2076-2615/10/4/628<i>Sillago sihama</i>hypoxia stress responsegene expressionRNA-Seq |
| spellingShingle | Wanida Saetan Changxu Tian Jiawang Yu Xinghua Lin Feixiang He Yang Huang Hongjuan Shi Yulei Zhang Guangli Li Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) Animals <i>Sillago sihama</i> hypoxia stress response gene expression RNA-Seq |
| title | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) |
| title_full | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) |
| title_fullStr | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) |
| title_full_unstemmed | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) |
| title_short | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (<i>Sillago sihama</i>) |
| title_sort | comparative transcriptome analysis of gill tissue in response to hypoxia in silver sillago i sillago sihama i |
| topic | <i>Sillago sihama</i> hypoxia stress response gene expression RNA-Seq |
| url | https://www.mdpi.com/2076-2615/10/4/628 |
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