Single-cell transcriptomic analysis of radiation-induced lung injury in rat
Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and e...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-03-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/10357 |
| _version_ | 1797228750055669760 |
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| author | Xing-Yuan Shi You-Qing Zhu Chan-Jin Liang Ting Chen Zhi Shi Wei Wang |
| author_facet | Xing-Yuan Shi You-Qing Zhu Chan-Jin Liang Ting Chen Zhi Shi Wei Wang |
| author_sort | Xing-Yuan Shi |
| collection | DOAJ |
| description |
Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and examined lung tissues by single-cell RNA sequencing (scRNA-seq) 2 weeks post-radiation. Analysis of lung tissues revealed 18 major cell populations, indicating an increase in cell-cell communication following radiation exposure. Neutrophils, macrophages, and monocytes displayed distinct subpopulations and uncovered potential for pro-inflammatory effects. Additionally, endothelial cells exhibited a highly inflammatory profile and the potential for reactive oxygen species (ROS) production. Furthermore, smooth muscle cells (SMC) showed a high propensity for extracellular matrix (ECM) deposition. Our findings broaden the current understanding of RILI and highlight potential avenues for further investigation and clinical applications.
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| first_indexed | 2024-04-24T15:01:39Z |
| format | Article |
| id | doaj.art-0d82f1eb766c4e62bbb04cb3f43de130 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T15:01:39Z |
| publishDate | 2024-03-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-0d82f1eb766c4e62bbb04cb3f43de1302024-04-02T14:48:50ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-03-0110.17305/bb.2024.10357Single-cell transcriptomic analysis of radiation-induced lung injury in ratXing-Yuan Shi0You-Qing Zhu1https://orcid.org/0009-0004-4846-2287Chan-Jin Liang2Ting Chen3Zhi Shi4https://orcid.org/0000-0002-8328-0305Wei Wang5Department of Radiation Oncology, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong, China; Department of Radiation Oncology, The Fifth Hospital of Guangzhou Medical University, Guangzhou, Guangdong, ChinaDepartment of Cell Biology and Institute of Biomedicine, Guangdong Provincial Biotechnology and Engineering Technology Research Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, Genomic Medicine Engineering Research Center of Ministry of Education, MOE Key Laboratory of Tumor Molecular Biology, National Engineering Research Center of Genetic Medicine, State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, China Department of Radiation Oncology, The Fifth Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China Department of Radiation Oncology, The Fifth Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China Department of Cell Biology and Institute of Biomedicine, Guangdong Provincial Biotechnology and Engineering Technology Research Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, Genomic Medicine Engineering Research Center of Ministry of Education, MOE Key Laboratory of Tumor Molecular Biology, National Engineering Research Center of Genetic Medicine, State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, China Department of Radiation Oncology, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong, China Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and examined lung tissues by single-cell RNA sequencing (scRNA-seq) 2 weeks post-radiation. Analysis of lung tissues revealed 18 major cell populations, indicating an increase in cell-cell communication following radiation exposure. Neutrophils, macrophages, and monocytes displayed distinct subpopulations and uncovered potential for pro-inflammatory effects. Additionally, endothelial cells exhibited a highly inflammatory profile and the potential for reactive oxygen species (ROS) production. Furthermore, smooth muscle cells (SMC) showed a high propensity for extracellular matrix (ECM) deposition. Our findings broaden the current understanding of RILI and highlight potential avenues for further investigation and clinical applications. https://www.bjbms.org/ojs/index.php/bjbms/article/view/10357Radiation-induced lung injury (RILI)single-cell RNA sequencing (scRNA-seq)inflammatoryradiation pneumonitiscell populations |
| spellingShingle | Xing-Yuan Shi You-Qing Zhu Chan-Jin Liang Ting Chen Zhi Shi Wei Wang Single-cell transcriptomic analysis of radiation-induced lung injury in rat Biomolecules & Biomedicine Radiation-induced lung injury (RILI) single-cell RNA sequencing (scRNA-seq) inflammatory radiation pneumonitis cell populations |
| title | Single-cell transcriptomic analysis of radiation-induced lung injury in rat |
| title_full | Single-cell transcriptomic analysis of radiation-induced lung injury in rat |
| title_fullStr | Single-cell transcriptomic analysis of radiation-induced lung injury in rat |
| title_full_unstemmed | Single-cell transcriptomic analysis of radiation-induced lung injury in rat |
| title_short | Single-cell transcriptomic analysis of radiation-induced lung injury in rat |
| title_sort | single cell transcriptomic analysis of radiation induced lung injury in rat |
| topic | Radiation-induced lung injury (RILI) single-cell RNA sequencing (scRNA-seq) inflammatory radiation pneumonitis cell populations |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/10357 |
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