Role of the visual experience-dependent nascent proteome in neuronal plasticity
Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenop...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2018-02-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/33420 |
| _version_ | 1828376076540182528 |
|---|---|
| author | Han-Hsuan Liu Daniel B McClatchy Lucio Schiapparelli Wanhua Shen John R Yates III Hollis T Cline |
| author_facet | Han-Hsuan Liu Daniel B McClatchy Lucio Schiapparelli Wanhua Shen John R Yates III Hollis T Cline |
| author_sort | Han-Hsuan Liu |
| collection | DOAJ |
| description | Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially synthesized candidate plasticity proteins (CPPs). The CPPs form strongly interconnected networks and are annotated to a variety of biological functions, including RNA splicing, protein translation, and chromatin remodeling. Functional analysis of select CPPs revealed the requirement for eukaryotic initiation factor three subunit A (eIF3A), fused in sarcoma (FUS), and ribosomal protein s17 (RPS17) in experience-dependent structural plasticity in tectal neurons and behavioral plasticity in tadpoles. These results demonstrate that the nascent proteome is dynamic in response to visual experience and that de novo synthesis of machinery that regulates RNA splicing and protein translation is required for experience-dependent plasticity. |
| first_indexed | 2024-04-14T07:54:25Z |
| format | Article |
| id | doaj.art-0d835f6178594afea8499c342d015dc9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:54:25Z |
| publishDate | 2018-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0d835f6178594afea8499c342d015dc92022-12-22T02:05:05ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.33420Role of the visual experience-dependent nascent proteome in neuronal plasticityHan-Hsuan Liu0https://orcid.org/0000-0002-5330-1689Daniel B McClatchy1https://orcid.org/0000-0002-0288-5645Lucio Schiapparelli2Wanhua Shen3John R Yates III4Hollis T Cline5https://orcid.org/0000-0002-4887-9603The Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, United States; Department of Neuroscience, The Scripps Research Institute, La Jolla, United States; Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, United StatesDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, United StatesThe Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, United States; Department of Neuroscience, The Scripps Research Institute, La Jolla, United StatesThe Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, United States; Department of Neuroscience, The Scripps Research Institute, La Jolla, United States; Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, ChinaDepartment of Neuroscience, The Scripps Research Institute, La Jolla, United States; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, United StatesThe Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, United States; Department of Neuroscience, The Scripps Research Institute, La Jolla, United States; Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, United States; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, United StatesExperience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response to visual experience in Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially synthesized candidate plasticity proteins (CPPs). The CPPs form strongly interconnected networks and are annotated to a variety of biological functions, including RNA splicing, protein translation, and chromatin remodeling. Functional analysis of select CPPs revealed the requirement for eukaryotic initiation factor three subunit A (eIF3A), fused in sarcoma (FUS), and ribosomal protein s17 (RPS17) in experience-dependent structural plasticity in tectal neurons and behavioral plasticity in tadpoles. These results demonstrate that the nascent proteome is dynamic in response to visual experience and that de novo synthesis of machinery that regulates RNA splicing and protein translation is required for experience-dependent plasticity.https://elifesciences.org/articles/33420nascent proteomeplasticityFUSvisual experienceeIF3AXenopus |
| spellingShingle | Han-Hsuan Liu Daniel B McClatchy Lucio Schiapparelli Wanhua Shen John R Yates III Hollis T Cline Role of the visual experience-dependent nascent proteome in neuronal plasticity eLife nascent proteome plasticity FUS visual experience eIF3A Xenopus |
| title | Role of the visual experience-dependent nascent proteome in neuronal plasticity |
| title_full | Role of the visual experience-dependent nascent proteome in neuronal plasticity |
| title_fullStr | Role of the visual experience-dependent nascent proteome in neuronal plasticity |
| title_full_unstemmed | Role of the visual experience-dependent nascent proteome in neuronal plasticity |
| title_short | Role of the visual experience-dependent nascent proteome in neuronal plasticity |
| title_sort | role of the visual experience dependent nascent proteome in neuronal plasticity |
| topic | nascent proteome plasticity FUS visual experience eIF3A Xenopus |
| url | https://elifesciences.org/articles/33420 |
| work_keys_str_mv | AT hanhsuanliu roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity AT danielbmcclatchy roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity AT lucioschiapparelli roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity AT wanhuashen roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity AT johnryatesiii roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity AT hollistcline roleofthevisualexperiencedependentnascentproteomeinneuronalplasticity |