Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides
Light-activated antisense oligonucleotides have been developed to induce gene knockdown in living cells, however, their synthesis remains challenging and application in cell-free systems is underexplored. Here, the authors report a one-step method for selectively attaching photocages onto phosphorot...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-04-01
|
| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-023-00860-2 |
| Summary: | Light-activated antisense oligonucleotides have been developed to induce gene knockdown in living cells, however, their synthesis remains challenging and application in cell-free systems is underexplored. Here, the authors report a one-step method for selectively attaching photocages onto phosphorothioate linkages of antisense oligonucleotides that can knockdown cell-free protein synthesis using light. |
|---|---|
| ISSN: | 2399-3669 |