Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides
Light-activated antisense oligonucleotides have been developed to induce gene knockdown in living cells, however, their synthesis remains challenging and application in cell-free systems is underexplored. Here, the authors report a one-step method for selectively attaching photocages onto phosphorot...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-04-01
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| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-023-00860-2 |
| _version_ | 1827974744650022912 |
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| author | Denis Hartmann Michael J. Booth |
| author_facet | Denis Hartmann Michael J. Booth |
| author_sort | Denis Hartmann |
| collection | DOAJ |
| description | Light-activated antisense oligonucleotides have been developed to induce gene knockdown in living cells, however, their synthesis remains challenging and application in cell-free systems is underexplored. Here, the authors report a one-step method for selectively attaching photocages onto phosphorothioate linkages of antisense oligonucleotides that can knockdown cell-free protein synthesis using light. |
| first_indexed | 2024-04-09T19:59:16Z |
| format | Article |
| id | doaj.art-0d84e3fda91a47cbae6150ab2ac6801c |
| institution | Directory Open Access Journal |
| issn | 2399-3669 |
| language | English |
| last_indexed | 2024-04-09T19:59:16Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj.art-0d84e3fda91a47cbae6150ab2ac6801c2023-04-03T05:19:28ZengNature PortfolioCommunications Chemistry2399-36692023-04-01611810.1038/s42004-023-00860-2Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotidesDenis Hartmann0Michael J. Booth1Department of Chemistry, University of OxfordDepartment of Chemistry, University of OxfordLight-activated antisense oligonucleotides have been developed to induce gene knockdown in living cells, however, their synthesis remains challenging and application in cell-free systems is underexplored. Here, the authors report a one-step method for selectively attaching photocages onto phosphorothioate linkages of antisense oligonucleotides that can knockdown cell-free protein synthesis using light.https://doi.org/10.1038/s42004-023-00860-2 |
| spellingShingle | Denis Hartmann Michael J. Booth Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides Communications Chemistry |
| title | Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides |
| title_full | Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides |
| title_fullStr | Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides |
| title_full_unstemmed | Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides |
| title_short | Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides |
| title_sort | accessible light controlled knockdown of cell free protein synthesis using phosphorothioate caged antisense oligonucleotides |
| url | https://doi.org/10.1038/s42004-023-00860-2 |
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