Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination
Summary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. Meiotic recombination is initiated by DNA double-strand break (DSB) followed by multiple processes of DNA repair. The exact mechanisms for how re...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720306392 |
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| author | Kazumasa Takemoto Naoki Tani Yuki Takada-Horisawa Sayoko Fujimura Nobuhiro Tanno Mariko Yamane Kaho Okamura Michihiko Sugimoto Kimi Araki Kei-ichiro Ishiguro |
| author_facet | Kazumasa Takemoto Naoki Tani Yuki Takada-Horisawa Sayoko Fujimura Nobuhiro Tanno Mariko Yamane Kaho Okamura Michihiko Sugimoto Kimi Araki Kei-ichiro Ishiguro |
| author_sort | Kazumasa Takemoto |
| collection | DOAJ |
| description | Summary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. Meiotic recombination is initiated by DNA double-strand break (DSB) followed by multiple processes of DNA repair. The exact mechanisms for how recombinases localize to DSB remain elusive. Here, we show that C19orf57/4930432K21Rik/BRME1 is a player for meiotic recombination in mice. C19orf57/4930432K21Rik/BRME1 associates with single-stranded DNA (ssDNA) binding proteins, BRCA2 and MEILB2/HSF2BP, which are critical recruiters of recombinases onto DSB sites. Disruption of C19orf57/4930432K21Rik/BRME1 shows severe impact on DSB repair and male fertility. Remarkably, removal of ssDNA binding proteins from DSB sites is delayed, and reciprocally, the loading of RAD51 and DMC1 onto resected ssDNA is impaired in Brme1 knockout (KO) spermatocytes. We propose that C19orf57/4930432K21Rik/BRME1 modulates localization of recombinases to meiotic DSB sites through the interaction with the BRCA2-MEILB2/HSF2BP complex during meiotic recombination. |
| first_indexed | 2024-12-11T02:08:39Z |
| format | Article |
| id | doaj.art-0d8530f0fc044b2790b7d99713c75123 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T02:08:39Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-0d8530f0fc044b2790b7d99713c751232022-12-22T01:24:19ZengElsevierCell Reports2211-12472020-05-01318Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic RecombinationKazumasa Takemoto0Naoki Tani1Yuki Takada-Horisawa2Sayoko Fujimura3Nobuhiro Tanno4Mariko Yamane5Kaho Okamura6Michihiko Sugimoto7Kimi Araki8Kei-ichiro Ishiguro9Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, Japan; Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanLiaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, JapanDepartment of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, JapanLiaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, JapanDepartment of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, JapanRIKEN, Center for Biosystems Dynamics Research, Kobe 650-0047, JapanDepartment of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, JapanInstitute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, JapanInstitute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, Japan; Center for Metabolic Regulation of Healthy Aging, Kumamoto University, Kumamoto 860-0811, JapanDepartment of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, Japan; Corresponding authorSummary: Meiotic recombination is critical for genetic exchange and generation of chiasmata that ensures faithful chromosome segregation during meiosis I. Meiotic recombination is initiated by DNA double-strand break (DSB) followed by multiple processes of DNA repair. The exact mechanisms for how recombinases localize to DSB remain elusive. Here, we show that C19orf57/4930432K21Rik/BRME1 is a player for meiotic recombination in mice. C19orf57/4930432K21Rik/BRME1 associates with single-stranded DNA (ssDNA) binding proteins, BRCA2 and MEILB2/HSF2BP, which are critical recruiters of recombinases onto DSB sites. Disruption of C19orf57/4930432K21Rik/BRME1 shows severe impact on DSB repair and male fertility. Remarkably, removal of ssDNA binding proteins from DSB sites is delayed, and reciprocally, the loading of RAD51 and DMC1 onto resected ssDNA is impaired in Brme1 knockout (KO) spermatocytes. We propose that C19orf57/4930432K21Rik/BRME1 modulates localization of recombinases to meiotic DSB sites through the interaction with the BRCA2-MEILB2/HSF2BP complex during meiotic recombination.http://www.sciencedirect.com/science/article/pii/S2211124720306392Meiosismeiotic recombinationRAD51DSBspermatogenesisChromosome |
| spellingShingle | Kazumasa Takemoto Naoki Tani Yuki Takada-Horisawa Sayoko Fujimura Nobuhiro Tanno Mariko Yamane Kaho Okamura Michihiko Sugimoto Kimi Araki Kei-ichiro Ishiguro Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination Cell Reports Meiosis meiotic recombination RAD51 DSB spermatogenesis Chromosome |
| title | Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination |
| title_full | Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination |
| title_fullStr | Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination |
| title_full_unstemmed | Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination |
| title_short | Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination |
| title_sort | meiosis specific c19orf57 4930432k21rik brme1 modulates localization of rad51 and dmc1 to dsbs in mouse meiotic recombination |
| topic | Meiosis meiotic recombination RAD51 DSB spermatogenesis Chromosome |
| url | http://www.sciencedirect.com/science/article/pii/S2211124720306392 |
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