Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years
Background Early detection of dysplastic changes within oral potentially malignant disorders is the mainstay to prevent oral cancer. Ki-67 is one of the most useful antigens in this purpose. Aims The study aims were to recognize and mutually compare the proliferative status of idiopathic oral leuko...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2020-06-01
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| Series: | South Asian Journal of Cancer |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1721212 |
| _version_ | 1819072455480705024 |
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| author | Krishnendu Mondal Rupali Mandal Badal Chandra Sarkar |
| author_facet | Krishnendu Mondal Rupali Mandal Badal Chandra Sarkar |
| author_sort | Krishnendu Mondal |
| collection | DOAJ |
| description | Background Early detection of dysplastic changes within oral potentially malignant disorders is the mainstay to prevent oral cancer. Ki-67 is one of the most useful antigens in this purpose.
Aims The study aims were to recognize and mutually compare the proliferative status of idiopathic oral leukoplakia (OL) patches, which presented through different forms of dysplasia and carcinoma.
Settings and Design In 4 years of observation, cumulatively 140 OL lesions were included for examination. The wholesome Ki-67 labeling scores in each of the subgroups were calculated.
Subjects and Methods The World Health Organization recommended histopathological classification was used to categorize the dysplastic and malignant lesions. Paraffin-embedded tissue sections were processed for Ki-67 immunostaining. The labeling indices (LIs) were quantified semiquantitatively at the site of maximal reactive cells on tissue sections.
Statistical Analysis The statistical comparison was performed by means of the SPSS software (Version 16.0 SPSS Inc.). A p-value < 0.05 was considered as the benchmark for statistical significance.
Results A steady and significant increment in Ki-67 expression was discovered from dysplastic to malignant OL patches compared with normal mucosa. The labeling differences were significant between normal mucosa and mild dysplasia, as well as between mild, moderate, and severe dysplasia. However, the expression did not differ significantly with the severity of oral cancers.
Conclusions Ki-67 is a useful molecular marker of carcinogenesis in OL. It also serves worthwhile in separating marginally dysplastic lesions, such as mild dysplasia or verrucous carcinoma from their benign epigones. |
| first_indexed | 2024-12-21T17:38:00Z |
| format | Article |
| id | doaj.art-0d8654138c7c479e939dc67de29544ad |
| institution | Directory Open Access Journal |
| issn | 2278-330X 2278-4306 |
| language | English |
| last_indexed | 2024-12-21T17:38:00Z |
| publishDate | 2020-06-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | South Asian Journal of Cancer |
| spelling | doaj.art-0d8654138c7c479e939dc67de29544ad2022-12-21T18:55:42ZengThieme Medical and Scientific Publishers Pvt. Ltd.South Asian Journal of Cancer2278-330X2278-43062020-06-0190209910410.1055/s-0040-1721212Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 YearsKrishnendu Mondal0Rupali Mandal1Badal Chandra Sarkar2Department of Pathology, North Bengal Medical College and Hospital, Darjeeling, West Bengal, IndiaDepartment of Pathology, North Bengal Medical College and Hospital, Darjeeling, West Bengal, IndiaDepartment of Oral Pathology, North Bengal Dental College and Hospital, Darjeeling, West Bengal, IndiaBackground Early detection of dysplastic changes within oral potentially malignant disorders is the mainstay to prevent oral cancer. Ki-67 is one of the most useful antigens in this purpose. Aims The study aims were to recognize and mutually compare the proliferative status of idiopathic oral leukoplakia (OL) patches, which presented through different forms of dysplasia and carcinoma. Settings and Design In 4 years of observation, cumulatively 140 OL lesions were included for examination. The wholesome Ki-67 labeling scores in each of the subgroups were calculated. Subjects and Methods The World Health Organization recommended histopathological classification was used to categorize the dysplastic and malignant lesions. Paraffin-embedded tissue sections were processed for Ki-67 immunostaining. The labeling indices (LIs) were quantified semiquantitatively at the site of maximal reactive cells on tissue sections. Statistical Analysis The statistical comparison was performed by means of the SPSS software (Version 16.0 SPSS Inc.). A p-value < 0.05 was considered as the benchmark for statistical significance. Results A steady and significant increment in Ki-67 expression was discovered from dysplastic to malignant OL patches compared with normal mucosa. The labeling differences were significant between normal mucosa and mild dysplasia, as well as between mild, moderate, and severe dysplasia. However, the expression did not differ significantly with the severity of oral cancers. Conclusions Ki-67 is a useful molecular marker of carcinogenesis in OL. It also serves worthwhile in separating marginally dysplastic lesions, such as mild dysplasia or verrucous carcinoma from their benign epigones.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1721212immunohistochemistryki-67oral epithelial dysplasiaoral leukoplakiasquamous cell carcinoma |
| spellingShingle | Krishnendu Mondal Rupali Mandal Badal Chandra Sarkar Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years South Asian Journal of Cancer immunohistochemistry ki-67 oral epithelial dysplasia oral leukoplakia squamous cell carcinoma |
| title | Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years |
| title_full | Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years |
| title_fullStr | Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years |
| title_full_unstemmed | Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years |
| title_short | Importance of Ki-67 Labeling in Oral Leukoplakia with Features of Dysplasia and Carcinomatous Transformation: An Observational Study over 4 Years |
| title_sort | importance of ki 67 labeling in oral leukoplakia with features of dysplasia and carcinomatous transformation an observational study over 4 years |
| topic | immunohistochemistry ki-67 oral epithelial dysplasia oral leukoplakia squamous cell carcinoma |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1721212 |
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