Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica)
Liberica coffee has an inferior quality when compared to arabica and robusta coffee. This flavour can be improved through a fermentation process involving indigenous microbes, one of which is a group of pectinolytic yeasts. Therefore, this study aimed to obtain yeast isolates with pectinolytic activ...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/10/bioconf_icgrc2024_01024.pdf |
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| author | Byagsa Yarisina Sunarharum Wenny Bekti Mahatmanto Tunjung Jatmiko Yoga Dwi |
| author_facet | Byagsa Yarisina Sunarharum Wenny Bekti Mahatmanto Tunjung Jatmiko Yoga Dwi |
| author_sort | Byagsa Yarisina |
| collection | DOAJ |
| description | Liberica coffee has an inferior quality when compared to arabica and robusta coffee. This flavour can be improved through a fermentation process involving indigenous microbes, one of which is a group of pectinolytic yeasts. Therefore, this study aimed to obtain yeast isolates with pectinolytic activity. Green bean samples were used for yeast isolation using YEPD agar media. Selected yeast isolates were screened for their pectinolytic activity semi-quantitatively using the well-diffusion method and quantitatively using the dinitrosalicylic acid (DNS) method. A total of six yeast isolates with different colony morphological characters were obtained and referred to as isolates Y1, Y2, Y3, Y4, Y5, and Y6. The isolates suspected to produce pectinase enzyme due to a clear zone around the well are Y2, Y3, Y4, and Y5. Y5 had the highest pectinolytic index, 022 and 0,73 on the second and fifth days of incubation, respectively. The four selected isolates were then screened quantitatively and showed that Y2 had the highest pectinase enzyme activity with a value of 12,66 ± 0,17 U/mL. The results showed that four yeast isolates from Liberica green coffee beans had pectinolytic activity with the highest enzyme activity found in Y2. |
| first_indexed | 2024-03-07T22:51:05Z |
| format | Article |
| id | doaj.art-0d91465a377d4af4a014a8c3c254c534 |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-03-07T22:51:05Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-0d91465a377d4af4a014a8c3c254c5342024-02-23T10:17:19ZengEDP SciencesBIO Web of Conferences2117-44582024-01-01910102410.1051/bioconf/20249101024bioconf_icgrc2024_01024Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica)Byagsa Yarisina0Sunarharum Wenny Bekti1Mahatmanto Tunjung2Jatmiko Yoga Dwi3Department of Biology. Faculty of Mathematics and Natural Sciences, Universitas BrawijayaDepartment of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas BrawijayaDepartment of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas BrawijayaDepartment of Biology. Faculty of Mathematics and Natural Sciences, Universitas BrawijayaLiberica coffee has an inferior quality when compared to arabica and robusta coffee. This flavour can be improved through a fermentation process involving indigenous microbes, one of which is a group of pectinolytic yeasts. Therefore, this study aimed to obtain yeast isolates with pectinolytic activity. Green bean samples were used for yeast isolation using YEPD agar media. Selected yeast isolates were screened for their pectinolytic activity semi-quantitatively using the well-diffusion method and quantitatively using the dinitrosalicylic acid (DNS) method. A total of six yeast isolates with different colony morphological characters were obtained and referred to as isolates Y1, Y2, Y3, Y4, Y5, and Y6. The isolates suspected to produce pectinase enzyme due to a clear zone around the well are Y2, Y3, Y4, and Y5. Y5 had the highest pectinolytic index, 022 and 0,73 on the second and fifth days of incubation, respectively. The four selected isolates were then screened quantitatively and showed that Y2 had the highest pectinase enzyme activity with a value of 12,66 ± 0,17 U/mL. The results showed that four yeast isolates from Liberica green coffee beans had pectinolytic activity with the highest enzyme activity found in Y2.https://www.bio-conferences.org/articles/bioconf/pdf/2024/10/bioconf_icgrc2024_01024.pdf |
| spellingShingle | Byagsa Yarisina Sunarharum Wenny Bekti Mahatmanto Tunjung Jatmiko Yoga Dwi Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) BIO Web of Conferences |
| title | Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) |
| title_full | Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) |
| title_fullStr | Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) |
| title_full_unstemmed | Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) |
| title_short | Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica) |
| title_sort | selection of pectinolytic yeast from liberica green coffee beans coffea liberica |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/10/bioconf_icgrc2024_01024.pdf |
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