Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice
High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wou...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
UIN Sunan Gunung Djati
2023-05-01
|
| Series: | Jurnal Biodjati |
| Subjects: | |
| Online Access: | https://journal.uinsgd.ac.id/index.php/biodjati/article/view/19968 |
| _version_ | 1827938831201992704 |
|---|---|
| author | Retno Susilowati Ulfa Maynisa Rohmanningrum |
| author_facet | Retno Susilowati Ulfa Maynisa Rohmanningrum |
| author_sort | Retno Susilowati |
| collection | DOAJ |
| description | High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. This study used three types of controls, namely KN (normal, HPMC 3%), K- (HPMC 3%), and K+ (Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on day 3, 7 and 14. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7 showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment. |
| first_indexed | 2024-03-13T08:44:39Z |
| format | Article |
| id | doaj.art-0d95a86d4c0f4e9eabfcac09dec267e2 |
| institution | Directory Open Access Journal |
| issn | 2541-4208 2548-1606 |
| language | English |
| last_indexed | 2024-03-13T08:44:39Z |
| publishDate | 2023-05-01 |
| publisher | UIN Sunan Gunung Djati |
| record_format | Article |
| series | Jurnal Biodjati |
| spelling | doaj.art-0d95a86d4c0f4e9eabfcac09dec267e22023-05-30T07:15:05ZengUIN Sunan Gunung DjatiJurnal Biodjati2541-42082548-16062023-05-018110611610.15575/biodjati.v8i1.199687325Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic MiceRetno Susilowati0Ulfa Maynisa Rohmanningrum1Departement of Magister of Biology, Faculty of Science and Technology, UIN Maulana Malik Ibrahim Malang, Jl. Gajayana No. 50, Malang, East Java, Indonesia, 65144Departement of Magister of Biology, Faculty of Science and Technology, UIN Maulana Malik Ibrahim Malang, Jl. Gajayana No. 50, Malang, East Java, Indonesia, 65144High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. This study used three types of controls, namely KN (normal, HPMC 3%), K- (HPMC 3%), and K+ (Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on day 3, 7 and 14. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7 showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment.https://journal.uinsgd.ac.id/index.php/biodjati/article/view/19968diabetes mellitus (dm), nigella sativa, trigonella foenum-graecum, wound healing |
| spellingShingle | Retno Susilowati Ulfa Maynisa Rohmanningrum Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice Jurnal Biodjati diabetes mellitus (dm), nigella sativa, trigonella foenum-graecum, wound healing |
| title | Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice |
| title_full | Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice |
| title_fullStr | Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice |
| title_full_unstemmed | Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice |
| title_short | Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice |
| title_sort | effective combination of nigella sativa and trigonella foenum graecum seed extract on wound healing in diabetic mice |
| topic | diabetes mellitus (dm), nigella sativa, trigonella foenum-graecum, wound healing |
| url | https://journal.uinsgd.ac.id/index.php/biodjati/article/view/19968 |
| work_keys_str_mv | AT retnosusilowati effectivecombinationofnigellasativaandtrigonellafoenumgraecumseedextractonwoundhealingindiabeticmice AT ulfamaynisarohmanningrum effectivecombinationofnigellasativaandtrigonellafoenumgraecumseedextractonwoundhealingindiabeticmice |