Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells
Thiazole derivatives have cytotoxic effects towards tumor cells, such as glioblastoma, melanoma, leukemia and lymphoma. However, the intracellular mechanism of this action is not clear. The aim of our study was to investigate the action of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-ca...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2020/04/Hreniukh_2_20.pdf |
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| author | V. P. Hreniukh N. S. Finiuk Ya. R. Shalai B. O. Manko B. V. Manko Yu. V. Ostapiuk O. R. Kulachkovskyy M. D. Obushak R. S. Stoika A. M. Babsky |
| author_facet | V. P. Hreniukh N. S. Finiuk Ya. R. Shalai B. O. Manko B. V. Manko Yu. V. Ostapiuk O. R. Kulachkovskyy M. D. Obushak R. S. Stoika A. M. Babsky |
| author_sort | V. P. Hreniukh |
| collection | DOAJ |
| description | Thiazole derivatives have cytotoxic effects towards tumor cells, such as glioblastoma, melanoma, leukemia and lymphoma. However, the intracellular mechanism of this action is not clear. The aim of our study was to investigate the action of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and 7-benzyl-8-methyl-2-propylpyrazolo[4,3-e]thiazolo[3,2-a]pyrimidin-4(2H)-one (PP2) on cellular structure, and bioenergetic functions of mitochondria in Nemeth-Kellner lymphoma cells (NK/Ly). The structure of treated NK/Ly cells and their mitochondria was examined using electron microscopy. The rate of oxygen uptake by isolated mitochondria was recorded by a polarographic method using a Clark electrode. The mitochondrial potential relative values were registered using fluorescence dye rhodamine 123. In the short-term (15 min), incubation with BF1 and PP2 in 10 and 50 µM concentrations induced apoptotic and necrotic changes in the structure of NK/Ly cells, such as fragmentation and disintegration of the nucleus, destruction of the plasma membrane, and an increase in numbers of lysosomes and mitochondria. A polarographic method did not show significant metabolic shifts in lymphoma mitochondria, in either in vitro or ex vivo actions of the thiazole derivatives. However, fluorescent microscopy showed a significant decrease in mitochondria potential, following a 15 min incubation of cells with 50 µM of PP2. Thus, the electron and fluorescent microscopy data suggest that mitochondria are involved in the mechanism of cytotoxic action of the studied thiazole derivatives. |
| first_indexed | 2024-03-09T08:45:31Z |
| format | Article |
| id | doaj.art-0d95acffb7b64a4e9267c50ffed7c816 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:45:31Z |
| publishDate | 2020-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-0d95acffb7b64a4e9267c50ffed7c8162023-12-02T15:28:16ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032020-04-0192212113010.15407/ubj92.02.121Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cellsV. P. Hreniukh0https://orcid.org/0000-0001-6418-2964N. S. Finiuk1https://orcid.org/0000-0002-4112-8815Ya. R. Shalai2B. O. Manko3B. V. Manko4Yu. V. Ostapiuk5https://orcid.org/0000-0001-7335-211XO. R. Kulachkovskyy6M. D. Obushak7https://orcid.org/0000-0001-8146-9529R. S. Stoika8https://orcid.org/0000-0001-5719-2187A. M. Babsky9https://orcid.org/0000-0001-5559-0456Ivan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, Ukraine; Institute of Cell Biology, Nationl Academy of Sciences of Ukraine, LvivIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, UkraineIvan Franko National University of Lviv, Ukraine; Institute of Cell Biology, Nationl Academy of Sciences of Ukraine, LvivIvan Franko National University of Lviv, UkraineThiazole derivatives have cytotoxic effects towards tumor cells, such as glioblastoma, melanoma, leukemia and lymphoma. However, the intracellular mechanism of this action is not clear. The aim of our study was to investigate the action of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and 7-benzyl-8-methyl-2-propylpyrazolo[4,3-e]thiazolo[3,2-a]pyrimidin-4(2H)-one (PP2) on cellular structure, and bioenergetic functions of mitochondria in Nemeth-Kellner lymphoma cells (NK/Ly). The structure of treated NK/Ly cells and their mitochondria was examined using electron microscopy. The rate of oxygen uptake by isolated mitochondria was recorded by a polarographic method using a Clark electrode. The mitochondrial potential relative values were registered using fluorescence dye rhodamine 123. In the short-term (15 min), incubation with BF1 and PP2 in 10 and 50 µM concentrations induced apoptotic and necrotic changes in the structure of NK/Ly cells, such as fragmentation and disintegration of the nucleus, destruction of the plasma membrane, and an increase in numbers of lysosomes and mitochondria. A polarographic method did not show significant metabolic shifts in lymphoma mitochondria, in either in vitro or ex vivo actions of the thiazole derivatives. However, fluorescent microscopy showed a significant decrease in mitochondria potential, following a 15 min incubation of cells with 50 µM of PP2. Thus, the electron and fluorescent microscopy data suggest that mitochondria are involved in the mechanism of cytotoxic action of the studied thiazole derivatives.http://ukrbiochemjournal.org/wp-content/uploads/2020/04/Hreniukh_2_20.pdflymphomamembrane potentialmitochondriathiazole derivatives |
| spellingShingle | V. P. Hreniukh N. S. Finiuk Ya. R. Shalai B. O. Manko B. V. Manko Yu. V. Ostapiuk O. R. Kulachkovskyy M. D. Obushak R. S. Stoika A. M. Babsky Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells The Ukrainian Biochemical Journal lymphoma membrane potential mitochondria thiazole derivatives |
| title | Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| title_full | Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| title_fullStr | Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| title_full_unstemmed | Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| title_short | Effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| title_sort | effects of thiazole derivatives on intracellular structure and functions in murine lymphoma cells |
| topic | lymphoma membrane potential mitochondria thiazole derivatives |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2020/04/Hreniukh_2_20.pdf |
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