MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO
Intro: Canine parvovirus type 2 (PVC-2), Protoparvovirus genus of the Parvoviridae family, is a worldwide distribution virus that affects the Canidae family. In free-living coyotes (Canis latrans), the presence of the PCV-2a, PCV-2b and PCV-2c subtypes of PVC-2 has been reported, but there are no re...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223004794 |
| _version_ | 1797824485921914880 |
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| author | S. Cedillo-Rosales D. Cedillo-Rosales A. Busqueta-Medina D. Avalos-Nolazco D. Zamora-Ávila J. Zaráte-Ramos R. Avalos-Ramirez |
| author_facet | S. Cedillo-Rosales D. Cedillo-Rosales A. Busqueta-Medina D. Avalos-Nolazco D. Zamora-Ávila J. Zaráte-Ramos R. Avalos-Ramirez |
| author_sort | S. Cedillo-Rosales |
| collection | DOAJ |
| description | Intro: Canine parvovirus type 2 (PVC-2), Protoparvovirus genus of the Parvoviridae family, is a worldwide distribution virus that affects the Canidae family. In free-living coyotes (Canis latrans), the presence of the PCV-2a, PCV-2b and PCV-2c subtypes of PVC-2 has been reported, but there are no reports of their presence as a cause of clinical damage. The objective of this study is to report the presence of PVC-2c in an outbreak of mild gastroenteritis in three coyote pups detected in northeastern Mexico Methods: During the fall of 2019, in the suburban area of Monterrey, N.L., 3 affected coyote pups were detected with a mild gastroenteric condition consisting of mild diarrhea with loose stools, vomiting, dehydration, loss of appetite, pale mucous membranes, and low weight. Stool samples were tested for Canine Parvovirus (CPV-2), Canine Coronavirus (CCV) or Giardia antigens with a commercial kit. All samples were positive for CPV-2 and these were subsequently analyzed by PCR and sequencing of the CPV-2 VP2 gene. Using bioinformatics, the VP2 gene sequence data obtained were used to establish phylogenetic relationships with homologous sequences reported in coyotes and CPV-2 vaccines. Findings: The genetic sequence of VP2 obtained showed a high homology (98.1 to 100%) with CPV-2c. The sequences obtained from the pups showed 100% homology to each other. The phylogenetic tree showed that the sequences reported in coyotes are grouped in different clades and that the sequence of the VP2 gene of CPV-2c from coyote pups is grouped in a different monophyletic group. Conclusion: Information suggests that wild coyotes may not only act as asymptomatic reservoir hosts but may also be clinically affected by PVC-2c. It is necessary to carry out studies to know the effects of the genetic subtypes of PVC-2 in the population of coyotes and other wild canids of northeastern Mexico. |
| first_indexed | 2024-03-13T10:39:41Z |
| format | Article |
| id | doaj.art-0d98b0849dea4fbe9cdd5e0712db64c0 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-13T10:39:41Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-0d98b0849dea4fbe9cdd5e0712db64c02023-05-18T04:39:06ZengElsevierInternational Journal of Infectious Diseases1201-97122023-05-01130S142S143MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICOS. Cedillo-Rosales0D. Cedillo-Rosales1A. Busqueta-Medina2D. Avalos-Nolazco3D. Zamora-Ávila4J. Zaráte-Ramos5R. Avalos-Ramirez6Autonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Laboratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Labaratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Laboratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Laboratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Labaratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Laboratory, General Escobedo, Nuevo León, MexicoAutonomous University of Nuevo León, Faculty of Veterinary Medicine and Zootechnics / Virology Labaratory, General Escobedo, Nuevo León, MexicoIntro: Canine parvovirus type 2 (PVC-2), Protoparvovirus genus of the Parvoviridae family, is a worldwide distribution virus that affects the Canidae family. In free-living coyotes (Canis latrans), the presence of the PCV-2a, PCV-2b and PCV-2c subtypes of PVC-2 has been reported, but there are no reports of their presence as a cause of clinical damage. The objective of this study is to report the presence of PVC-2c in an outbreak of mild gastroenteritis in three coyote pups detected in northeastern Mexico Methods: During the fall of 2019, in the suburban area of Monterrey, N.L., 3 affected coyote pups were detected with a mild gastroenteric condition consisting of mild diarrhea with loose stools, vomiting, dehydration, loss of appetite, pale mucous membranes, and low weight. Stool samples were tested for Canine Parvovirus (CPV-2), Canine Coronavirus (CCV) or Giardia antigens with a commercial kit. All samples were positive for CPV-2 and these were subsequently analyzed by PCR and sequencing of the CPV-2 VP2 gene. Using bioinformatics, the VP2 gene sequence data obtained were used to establish phylogenetic relationships with homologous sequences reported in coyotes and CPV-2 vaccines. Findings: The genetic sequence of VP2 obtained showed a high homology (98.1 to 100%) with CPV-2c. The sequences obtained from the pups showed 100% homology to each other. The phylogenetic tree showed that the sequences reported in coyotes are grouped in different clades and that the sequence of the VP2 gene of CPV-2c from coyote pups is grouped in a different monophyletic group. Conclusion: Information suggests that wild coyotes may not only act as asymptomatic reservoir hosts but may also be clinically affected by PVC-2c. It is necessary to carry out studies to know the effects of the genetic subtypes of PVC-2 in the population of coyotes and other wild canids of northeastern Mexico.http://www.sciencedirect.com/science/article/pii/S1201971223004794 |
| spellingShingle | S. Cedillo-Rosales D. Cedillo-Rosales A. Busqueta-Medina D. Avalos-Nolazco D. Zamora-Ávila J. Zaráte-Ramos R. Avalos-Ramirez MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO International Journal of Infectious Diseases |
| title | MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO |
| title_full | MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO |
| title_fullStr | MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO |
| title_full_unstemmed | MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO |
| title_short | MOLECULAR DETECTION OF CANINE PARVOVIRUS-2C (CPV-2C) IN DISEASED COYOTE PUPS (CANIS LATRANS) AT NORTHEASTERN MEXICO |
| title_sort | molecular detection of canine parvovirus 2c cpv 2c in diseased coyote pups canis latrans at northeastern mexico |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223004794 |
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