Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts
In this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10<sup>−15</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/3/89 |
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| author | Almaz Kamalov Mikhail Shishov Natalia Smirnova Vera Kodolova-Chukhontseva Irina Dobrovol’skaya Konstantin Kolbe Andrei Didenko Elena Ivan’kova Vladimir Yudin Pierfrancesco Morganti |
| author_facet | Almaz Kamalov Mikhail Shishov Natalia Smirnova Vera Kodolova-Chukhontseva Irina Dobrovol’skaya Konstantin Kolbe Andrei Didenko Elena Ivan’kova Vladimir Yudin Pierfrancesco Morganti |
| author_sort | Almaz Kamalov |
| collection | DOAJ |
| description | In this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10<sup>−15</sup> to 10<sup>2</sup> S/m with increasing graphene content (from 0 to 5.0 wt.%). The characteristics of ionic and electronic currents flowing through the matrix with the superposition of cyclic potentials ± 100 mV were studied. The high stability of the composite was established during prolonged cycling (130 h) in an electric field with a frequency of 0.016 Hz. It was established that the composite films based on polyimide and graphene have good biocompatibility and are not toxic to fibroblast cells. It was shown that preliminary electrical stimulation increases the proliferative activity of human dermal fibroblasts in comparison with intact cells. It is revealed that an electric field with a strength E = 0.02–0.04 V/m applied to the polyimide films containing 0.5–3.0 wt.% of the graphene nanoparticles activates cellular processes (adhesion, proliferation). |
| first_indexed | 2024-03-09T23:34:44Z |
| format | Article |
| id | doaj.art-0d9ab9440c4442bea49955659d790388 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T23:34:44Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-0d9ab9440c4442bea49955659d7903882023-11-23T17:02:50ZengMDPI AGJournal of Functional Biomaterials2079-49832022-06-011338910.3390/jfb13030089Influence of Electric Field on Proliferation Activity of Human Dermal FibroblastsAlmaz Kamalov0Mikhail Shishov1Natalia Smirnova2Vera Kodolova-Chukhontseva3Irina Dobrovol’skaya4Konstantin Kolbe5Andrei Didenko6Elena Ivan’kova7Vladimir Yudin8Pierfrancesco Morganti9Research Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaResearch Laboratory “Polymer Materials for Tissue Engineering and Transplantology”, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, 195251 St. Petersburg, RussiaR&D Unit, Academy of History of Healthcare Art, Lungotevere in Sassia 3, 00186 Rome, ItalyIn this work, an electrically conductive composite based on thermoplastic polyimide and graphene was obtained and used as a bioelectrode for electrical stimulation of human dermal fibroblasts. The values of the electrical conductivity of the obtained composite films varied from 10<sup>−15</sup> to 10<sup>2</sup> S/m with increasing graphene content (from 0 to 5.0 wt.%). The characteristics of ionic and electronic currents flowing through the matrix with the superposition of cyclic potentials ± 100 mV were studied. The high stability of the composite was established during prolonged cycling (130 h) in an electric field with a frequency of 0.016 Hz. It was established that the composite films based on polyimide and graphene have good biocompatibility and are not toxic to fibroblast cells. It was shown that preliminary electrical stimulation increases the proliferative activity of human dermal fibroblasts in comparison with intact cells. It is revealed that an electric field with a strength E = 0.02–0.04 V/m applied to the polyimide films containing 0.5–3.0 wt.% of the graphene nanoparticles activates cellular processes (adhesion, proliferation).https://www.mdpi.com/2079-4983/13/3/89polyimidegraphenefibroblastselectrostimulationskin |
| spellingShingle | Almaz Kamalov Mikhail Shishov Natalia Smirnova Vera Kodolova-Chukhontseva Irina Dobrovol’skaya Konstantin Kolbe Andrei Didenko Elena Ivan’kova Vladimir Yudin Pierfrancesco Morganti Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts Journal of Functional Biomaterials polyimide graphene fibroblasts electrostimulation skin |
| title | Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts |
| title_full | Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts |
| title_fullStr | Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts |
| title_full_unstemmed | Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts |
| title_short | Influence of Electric Field on Proliferation Activity of Human Dermal Fibroblasts |
| title_sort | influence of electric field on proliferation activity of human dermal fibroblasts |
| topic | polyimide graphene fibroblasts electrostimulation skin |
| url | https://www.mdpi.com/2079-4983/13/3/89 |
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