Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction
The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and puri...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/19/3938 |
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| author | Gabriela Montiel-Jarillo Diego A. Morales-Urrea Edgardo M. Contreras Alex López-Córdoba Edwin Yesid Gómez-Pachón Julián Carrera María Eugenia Suárez-Ojeda |
| author_facet | Gabriela Montiel-Jarillo Diego A. Morales-Urrea Edgardo M. Contreras Alex López-Córdoba Edwin Yesid Gómez-Pachón Julián Carrera María Eugenia Suárez-Ojeda |
| author_sort | Gabriela Montiel-Jarillo |
| collection | DOAJ |
| description | The use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB. |
| first_indexed | 2024-03-09T21:16:34Z |
| format | Article |
| id | doaj.art-0d9b4d38fe3b488a8d49b62d51b6257f |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T21:16:34Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-0d9b4d38fe3b488a8d49b62d51b6257f2023-11-23T21:31:33ZengMDPI AGPolymers2073-43602022-09-011419393810.3390/polym14193938Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent ExtractionGabriela Montiel-Jarillo0Diego A. Morales-Urrea1Edgardo M. Contreras2Alex López-Córdoba3Edwin Yesid Gómez-Pachón4Julián Carrera5María Eugenia Suárez-Ojeda6GENOCOV Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Escola d’Enginyeria. Edifici Q Campus UAB, Bellaterra, 08193 Barcelona, SpainDivisión Catalizadores y Superficies, Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (CONICET), Av. Colón 10850, Mar del Plata 7600, ArgentinaDivisión Catalizadores y Superficies, Instituto de Investigaciones en Ciencia y Tecnología de Materiales, INTEMA (CONICET), Av. Colón 10850, Mar del Plata 7600, ArgentinaGrupo de Investigación en Bioeconomía y Sostenibilidad Agroalimentaria, Escuela de Administración de Empresas Agropecuarias, Facultad Seccional Duitama, Universidad Pedagógica y Tecnológica de Colombia, Carrera 18 con Calle 22, Duitama 150461, ColombiaGrupo de Investigación en Diseño, Innovación y Asistencia Técnica de Materiales Avanzados-DITMAV, Escuela de Diseño Industrial, Universidad Pedagógica y Tecnológica de Colombia-UPTC, Duitama 150461, ColombiaGENOCOV Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Escola d’Enginyeria. Edifici Q Campus UAB, Bellaterra, 08193 Barcelona, SpainGENOCOV Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Universitat Autònoma de Barcelona, Escola d’Enginyeria. Edifici Q Campus UAB, Bellaterra, 08193 Barcelona, SpainThe use of mixed microbial cultures (MMC) and organic wastes and wastewaters as feed sources is considered an appealing approach to reduce the current polyhydroxyalkanoates (PHAs) production costs. However, this method entails an additional hurdle to the PHAs downstream processing (recovery and purification). In the current work, the effect of a sodium hypochlorite (NaClO) pre-treatment coupled with dimethyl carbonate (DMC) or chloroform (CF) as extraction solvents on the PHAs recovery efficiency (RE) from MMC was evaluated. MMC were harvested from a sequencing batch reactor (SBR) fed with a synthetic prefermented olive mill wastewaster. Two different carbon-sources (acetic acid and acetic/propionic acids) were employed during the batch accumulation of polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from MMC. Obtained PHAs were characterized by <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance, gel-permeation chromatography, differential scanning calorimetry, and thermal gravimetric analysis. The results showed that when a NaClO pre-treatment is not added, the use of DMC allows to obtain higher RE of both biopolymers (PHB and PHBV), in comparison with CF. In contrast, the use of CF as extraction solvent required a pre-treatment step to improve the PHB and PHBV recovery. In all cases, RE values were higher for PHBV than for PHB.https://www.mdpi.com/2073-4360/14/19/3938polyhydroxyalkanoatesmixed microbial culturesextractionsodium hypochloritedimethyl carbonatechloroform |
| spellingShingle | Gabriela Montiel-Jarillo Diego A. Morales-Urrea Edgardo M. Contreras Alex López-Córdoba Edwin Yesid Gómez-Pachón Julián Carrera María Eugenia Suárez-Ojeda Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction Polymers polyhydroxyalkanoates mixed microbial cultures extraction sodium hypochlorite dimethyl carbonate chloroform |
| title | Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction |
| title_full | Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction |
| title_fullStr | Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction |
| title_full_unstemmed | Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction |
| title_short | Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction |
| title_sort | improvement of the polyhydroxyalkanoates recovery from mixed microbial cultures using sodium hypochlorite pre treatment coupled with solvent extraction |
| topic | polyhydroxyalkanoates mixed microbial cultures extraction sodium hypochlorite dimethyl carbonate chloroform |
| url | https://www.mdpi.com/2073-4360/14/19/3938 |
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