Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model.
Exosomes are endosome-derived small membrane vesicles that are secreted by most cell types including tumor cells. Tumor-derived exosomes usually contain tumor antigens and have been used as a source of tumor antigens to stimulate anti-tumor immune responses. However, many reports also suggest that t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3149056?pdf=render |
| _version_ | 1818128212349157376 |
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| author | Chenjie Yang Seon-Hee Kim Nicole R Bianco Paul D Robbins |
| author_facet | Chenjie Yang Seon-Hee Kim Nicole R Bianco Paul D Robbins |
| author_sort | Chenjie Yang |
| collection | DOAJ |
| description | Exosomes are endosome-derived small membrane vesicles that are secreted by most cell types including tumor cells. Tumor-derived exosomes usually contain tumor antigens and have been used as a source of tumor antigens to stimulate anti-tumor immune responses. However, many reports also suggest that tumor-derived exosomes can facilitate tumor immune evasion through different mechanisms, most of which are antigen-independent. In the present study we used a mouse model of delayed-type hypersensitivity (DTH) and demonstrated that local administration of tumor-derived exosomes carrying the model antigen chicken ovalbumin (OVA) resulted in the suppression of DTH response in an antigen-specific manner. Analysis of exosome trafficking demonstrated that following local injection, tumor-derived exosomes were internalized by CD11c+ cells and transported to the draining LN. Exosome-mediated DTH suppression is associated with increased mRNA levels of TGF-β1 and IL-4 in the draining LN. The tumor-derived exosomes examined were also found to inhibit DC maturation. Taken together, our results suggest a role for tumor-derived exosomes in inducing tumor antigen-specific immunosuppression, possibly by modulating the function of APCs. |
| first_indexed | 2024-12-11T07:29:40Z |
| format | Article |
| id | doaj.art-0d9c2dfe61a84b51b857aeab2332fe79 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:29:40Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0d9c2dfe61a84b51b857aeab2332fe792022-12-22T01:15:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2251710.1371/journal.pone.0022517Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model.Chenjie YangSeon-Hee KimNicole R BiancoPaul D RobbinsExosomes are endosome-derived small membrane vesicles that are secreted by most cell types including tumor cells. Tumor-derived exosomes usually contain tumor antigens and have been used as a source of tumor antigens to stimulate anti-tumor immune responses. However, many reports also suggest that tumor-derived exosomes can facilitate tumor immune evasion through different mechanisms, most of which are antigen-independent. In the present study we used a mouse model of delayed-type hypersensitivity (DTH) and demonstrated that local administration of tumor-derived exosomes carrying the model antigen chicken ovalbumin (OVA) resulted in the suppression of DTH response in an antigen-specific manner. Analysis of exosome trafficking demonstrated that following local injection, tumor-derived exosomes were internalized by CD11c+ cells and transported to the draining LN. Exosome-mediated DTH suppression is associated with increased mRNA levels of TGF-β1 and IL-4 in the draining LN. The tumor-derived exosomes examined were also found to inhibit DC maturation. Taken together, our results suggest a role for tumor-derived exosomes in inducing tumor antigen-specific immunosuppression, possibly by modulating the function of APCs.http://europepmc.org/articles/PMC3149056?pdf=render |
| spellingShingle | Chenjie Yang Seon-Hee Kim Nicole R Bianco Paul D Robbins Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. PLoS ONE |
| title | Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. |
| title_full | Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. |
| title_fullStr | Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. |
| title_full_unstemmed | Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. |
| title_short | Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model. |
| title_sort | tumor derived exosomes confer antigen specific immunosuppression in a murine delayed type hypersensitivity model |
| url | http://europepmc.org/articles/PMC3149056?pdf=render |
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