Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure
Grapevine trunk diseases (GTDs) are a substantial challenge to viticulture, especially with a lack of available control measures. The lack of approved fungicides necessitates the exploration of alternative controls. One promising approach is the investigation of disease escape plants, which remain h...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1231832/full |
| _version_ | 1797738231276503040 |
|---|---|
| author | Damola O. Adejoro E. Eirian Jones Hayley J. Ridgway Hayley J. Ridgway Dion C. Mundy Bhanupratap R. Vanga Bhanupratap R. Vanga Simon R. Bulman |
| author_facet | Damola O. Adejoro E. Eirian Jones Hayley J. Ridgway Hayley J. Ridgway Dion C. Mundy Bhanupratap R. Vanga Bhanupratap R. Vanga Simon R. Bulman |
| author_sort | Damola O. Adejoro |
| collection | DOAJ |
| description | Grapevine trunk diseases (GTDs) are a substantial challenge to viticulture, especially with a lack of available control measures. The lack of approved fungicides necessitates the exploration of alternative controls. One promising approach is the investigation of disease escape plants, which remain healthy under high disease pressure, likely due to their microbiome function. This study explored the microbiome of grapevines with the disease escape phenotype. DNA metabarcoding of the ribosomal internal transcribed spacer 1 (ITS1) and 16S ribosomal RNA gene was applied to trunk tissues of GTD escape and adjacent diseased vines. Our findings showed that the GTD escape vines had a significantly different microbiome compared with diseased vines. The GTD escape vines consistently harbored a higher relative abundance of the bacterial taxa Pseudomonas and Hymenobacter. Among fungi, Aureobasidium and Rhodotorula were differentially associated with GTD escape vines, while the GTD pathogen, Eutypa, was associated with the diseased vines. This is the first report of the link between the GTD escape phenotype and the grapevine microbiome. |
| first_indexed | 2024-03-12T13:39:53Z |
| format | Article |
| id | doaj.art-0d9fb52c2f484ca487680daee24e2fd1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:39:53Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0d9fb52c2f484ca487680daee24e2fd12023-08-23T19:45:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12318321231832Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structureDamola O. Adejoro0E. Eirian Jones1Hayley J. Ridgway2Hayley J. Ridgway3Dion C. Mundy4Bhanupratap R. Vanga5Bhanupratap R. Vanga6Simon R. Bulman7Department of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Canterbury, New ZealandDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Canterbury, New ZealandDepartment of Pest-Management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Canterbury, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Lincoln, Canterbury, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Blenheim, Marlborough, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Lincoln, Canterbury, New ZealandGrapevine Improvement Laboratory, Bragato Research Institute, Lincoln, Canterbury, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Lincoln, Canterbury, New ZealandGrapevine trunk diseases (GTDs) are a substantial challenge to viticulture, especially with a lack of available control measures. The lack of approved fungicides necessitates the exploration of alternative controls. One promising approach is the investigation of disease escape plants, which remain healthy under high disease pressure, likely due to their microbiome function. This study explored the microbiome of grapevines with the disease escape phenotype. DNA metabarcoding of the ribosomal internal transcribed spacer 1 (ITS1) and 16S ribosomal RNA gene was applied to trunk tissues of GTD escape and adjacent diseased vines. Our findings showed that the GTD escape vines had a significantly different microbiome compared with diseased vines. The GTD escape vines consistently harbored a higher relative abundance of the bacterial taxa Pseudomonas and Hymenobacter. Among fungi, Aureobasidium and Rhodotorula were differentially associated with GTD escape vines, while the GTD pathogen, Eutypa, was associated with the diseased vines. This is the first report of the link between the GTD escape phenotype and the grapevine microbiome.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1231832/fullVitis viniferamicrobiomePseudomonasgrapevine trunk diseasesEutypadisease escape |
| spellingShingle | Damola O. Adejoro E. Eirian Jones Hayley J. Ridgway Hayley J. Ridgway Dion C. Mundy Bhanupratap R. Vanga Bhanupratap R. Vanga Simon R. Bulman Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure Frontiers in Microbiology Vitis vinifera microbiome Pseudomonas grapevine trunk diseases Eutypa disease escape |
| title | Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure |
| title_full | Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure |
| title_fullStr | Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure |
| title_full_unstemmed | Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure |
| title_short | Grapevines escaping trunk diseases in New Zealand vineyards have a distinct microbiome structure |
| title_sort | grapevines escaping trunk diseases in new zealand vineyards have a distinct microbiome structure |
| topic | Vitis vinifera microbiome Pseudomonas grapevine trunk diseases Eutypa disease escape |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1231832/full |
| work_keys_str_mv | AT damolaoadejoro grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT eeirianjones grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT hayleyjridgway grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT hayleyjridgway grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT dioncmundy grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT bhanuprataprvanga grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT bhanuprataprvanga grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure AT simonrbulman grapevinesescapingtrunkdiseasesinnewzealandvineyardshaveadistinctmicrobiomestructure |