Microflow Cytometers with Integrated Hydrodynamic Focusing
This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-04-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/4/4674 |
| _version_ | 1811263258074546176 |
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| author | Martin Schmidt Nicole Bock Hülya Ragusch Peter Simon Andreas Kummrow Janko Theisen Marcin Frankowski Jörg Neukammer |
| author_facet | Martin Schmidt Nicole Bock Hülya Ragusch Peter Simon Andreas Kummrow Janko Theisen Marcin Frankowski Jörg Neukammer |
| author_sort | Martin Schmidt |
| collection | DOAJ |
| description | This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension of micro particles or blood cells—is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood. |
| first_indexed | 2024-04-12T19:41:36Z |
| format | Article |
| id | doaj.art-0da13722a9aa4e23bbf2c09811bd39a2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T19:41:36Z |
| publishDate | 2013-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-0da13722a9aa4e23bbf2c09811bd39a22022-12-22T03:19:04ZengMDPI AGSensors1424-82202013-04-011344674469310.3390/s130404674Microflow Cytometers with Integrated Hydrodynamic FocusingMartin SchmidtNicole BockHülya RaguschPeter SimonAndreas KummrowJanko TheisenMarcin FrankowskiJörg NeukammerThis study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension of micro particles or blood cells—is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood.http://www.mdpi.com/1424-8220/13/4/4674microfluidicsflow cytometrylab-on-a-chipsingle cell analysisblood cell differentiationhydrodynamic focusingultraprecision milling |
| spellingShingle | Martin Schmidt Nicole Bock Hülya Ragusch Peter Simon Andreas Kummrow Janko Theisen Marcin Frankowski Jörg Neukammer Microflow Cytometers with Integrated Hydrodynamic Focusing Sensors microfluidics flow cytometry lab-on-a-chip single cell analysis blood cell differentiation hydrodynamic focusing ultraprecision milling |
| title | Microflow Cytometers with Integrated Hydrodynamic Focusing |
| title_full | Microflow Cytometers with Integrated Hydrodynamic Focusing |
| title_fullStr | Microflow Cytometers with Integrated Hydrodynamic Focusing |
| title_full_unstemmed | Microflow Cytometers with Integrated Hydrodynamic Focusing |
| title_short | Microflow Cytometers with Integrated Hydrodynamic Focusing |
| title_sort | microflow cytometers with integrated hydrodynamic focusing |
| topic | microfluidics flow cytometry lab-on-a-chip single cell analysis blood cell differentiation hydrodynamic focusing ultraprecision milling |
| url | http://www.mdpi.com/1424-8220/13/4/4674 |
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