Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally
In vitro culture allows the production of numerous plants with both desirable and undesirable traits. To investigate the impact of the propagation method on highbush blueberry plants, an analysis was performed on four groups of differentially propagated plants: in vitro with axillary (TC-Ax) or adve...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| author | Marzena Mazurek Aleksandra Siekierzyńska Tomasz Piechowiak Anna Spinardi Wojciech Litwińczuk |
| author_facet | Marzena Mazurek Aleksandra Siekierzyńska Tomasz Piechowiak Anna Spinardi Wojciech Litwińczuk |
| author_sort | Marzena Mazurek |
| collection | DOAJ |
| description | In vitro culture allows the production of numerous plants with both desirable and undesirable traits. To investigate the impact of the propagation method on highbush blueberry plants, an analysis was performed on four groups of differentially propagated plants: in vitro with axillary (TC-Ax) or adventitious shoots (TC-Ad), conventionally (SC) and using a mixed method (TC/SC). The analysis included plant features (shoot length and branching, chlorophyll and fluorescence and DNA methylation) and fruit properties (antioxidant compounds). The data obtained indicated significant differences between plants propagated conventionally and in vitro, as well as variations among plants derived from in vitro cultures with different types of explants. SC plants generally exhibited the lowest values of morphological and physiological parameters but produced fruits richest in antioxidant compounds. TC/SC plants were dominant in length, branching and fluorescence. Conversely, TC-Ax plants produced fruits with the lowest levels of antioxidant compounds. The methylation-sensitive amplified polymorphism (MSAP) technique was employed to detect molecular differences. TC-Ad plants showed the highest methylation level, whereas SC plants had the lowest. The overall methylation level varied among differentially propagated plants. It can be speculated that the differences among the analysed plants may be attributed to variations in DNA methylation. |
| first_indexed | 2024-03-08T15:04:30Z |
| format | Article |
| id | doaj.art-0da215d4b72c45a1ae40d0380d609cd8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:04:30Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0da215d4b72c45a1ae40d0380d609cd82024-01-10T14:59:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125154410.3390/ijms25010544Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and ConventionallyMarzena Mazurek0Aleksandra Siekierzyńska1Tomasz Piechowiak2Anna Spinardi3Wojciech Litwińczuk4Department of Physiology and Plant Biotechnology, Institute of Agricultural Sciences, Environment Management and Protection University of Rzeszow, Ćwiklińskiej 2, 35-601 Rzeszow, PolandDepartment of Physiology and Plant Biotechnology, Institute of Agricultural Sciences, Environment Management and Protection University of Rzeszow, Ćwiklińskiej 2, 35-601 Rzeszow, PolandDepartment of Chemistry and Food Toxicology, Institute of Food Technology and Nutrition, University of Rzeszow, St. Cwiklinskiej 1a, 35-601 Rzeszow, PolandDepartment of Agricultural and Environmental Sciences—Production, Landscape, Agroenergy, Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Physiology and Plant Biotechnology, Institute of Agricultural Sciences, Environment Management and Protection University of Rzeszow, Ćwiklińskiej 2, 35-601 Rzeszow, PolandIn vitro culture allows the production of numerous plants with both desirable and undesirable traits. To investigate the impact of the propagation method on highbush blueberry plants, an analysis was performed on four groups of differentially propagated plants: in vitro with axillary (TC-Ax) or adventitious shoots (TC-Ad), conventionally (SC) and using a mixed method (TC/SC). The analysis included plant features (shoot length and branching, chlorophyll and fluorescence and DNA methylation) and fruit properties (antioxidant compounds). The data obtained indicated significant differences between plants propagated conventionally and in vitro, as well as variations among plants derived from in vitro cultures with different types of explants. SC plants generally exhibited the lowest values of morphological and physiological parameters but produced fruits richest in antioxidant compounds. TC/SC plants were dominant in length, branching and fluorescence. Conversely, TC-Ax plants produced fruits with the lowest levels of antioxidant compounds. The methylation-sensitive amplified polymorphism (MSAP) technique was employed to detect molecular differences. TC-Ad plants showed the highest methylation level, whereas SC plants had the lowest. The overall methylation level varied among differentially propagated plants. It can be speculated that the differences among the analysed plants may be attributed to variations in DNA methylation.https://www.mdpi.com/1422-0067/25/1/544plant in vitro culturesomaclonal variation<i>Vaccinium</i> sp.DNA methylationantioxidant |
| spellingShingle | Marzena Mazurek Aleksandra Siekierzyńska Tomasz Piechowiak Anna Spinardi Wojciech Litwińczuk Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally International Journal of Molecular Sciences plant in vitro culture somaclonal variation <i>Vaccinium</i> sp. DNA methylation antioxidant |
| title | Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally |
| title_full | Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally |
| title_fullStr | Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally |
| title_full_unstemmed | Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally |
| title_short | Comprehensive Analysis of Highbush Blueberry Plants Propagated In Vitro and Conventionally |
| title_sort | comprehensive analysis of highbush blueberry plants propagated in vitro and conventionally |
| topic | plant in vitro culture somaclonal variation <i>Vaccinium</i> sp. DNA methylation antioxidant |
| url | https://www.mdpi.com/1422-0067/25/1/544 |
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