Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China
The genus <i>Hepacivirus</i> contains single-stranded positive-sense RNA viruses belonging to the family <i>Flaviviridae</i>, which comprises 14 species. These 14 hepaciviruses have been found in different mammals, such as primates, dogs, bats, and rodents. To date, <i>...
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MDPI AG
2023-11-01
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| Serie: | Pathogens |
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| Länkar: | https://www.mdpi.com/2076-0817/12/12/1400 |
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| author | Ling Guo Bei Li Peiyu Han Na Dong Yan Zhu Fuli Li Haorui Si Zhengli Shi Bo Wang Xinglou Yang Yunzhi Zhang |
| author_facet | Ling Guo Bei Li Peiyu Han Na Dong Yan Zhu Fuli Li Haorui Si Zhengli Shi Bo Wang Xinglou Yang Yunzhi Zhang |
| author_sort | Ling Guo |
| collection | DOAJ |
| description | The genus <i>Hepacivirus</i> contains single-stranded positive-sense RNA viruses belonging to the family <i>Flaviviridae</i>, which comprises 14 species. These 14 hepaciviruses have been found in different mammals, such as primates, dogs, bats, and rodents. To date, <i>Hepacivirus</i> has not been reported in the shrew genus of <i>Crocidura</i>. To study the prevalence and genetic evolution of <i>Hepacivirus</i> in small mammals in Yunnan Province, China, molecular detection of <i>Hepacivirus</i> in small mammals from Yunnan Province during 2016 and 2017 was performed using reverse-transcription polymerase chain reaction (RT-PCR). Our results showed that the overall infection rate of <i>Hepacivirus</i> in small mammals was 0.12% (2/1602), and the host animal was the Southeast Asian shrew (<i>Crocidura fuliginosa</i>) (12.5%, 2/16). Quantitative real-time PCR showed that <i>Hepacivirus</i> had the highest viral RNA copy number in the liver. Phylogenetic analysis revealed that the hepaciviruses obtained in this study does not belong to any designated species of hepaciviruses and forms an independent clade. To conclude, a novel hepacivirus was identified for the first time in <i>C. fuliginosa</i> specimens from Yunnan Province, China. This study expands the host range and viral diversity of hepaciviruses. |
| first_indexed | 2024-03-08T20:27:39Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-08T20:27:39Z |
| publishDate | 2023-11-01 |
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| series | Pathogens |
| spelling | doaj.art-0da217f5d09b44d1b3aceb8fa25ca87c2023-12-22T14:30:49ZengMDPI AGPathogens2076-08172023-11-011212140010.3390/pathogens12121400Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, ChinaLing Guo0Bei Li1Peiyu Han2Na Dong3Yan Zhu4Fuli Li5Haorui Si6Zhengli Shi7Bo Wang8Xinglou Yang9Yunzhi Zhang10Yunnan Province Key Laboratory of Anti-Pathogenic Plant Resources Screening, Yunnan Province Key University Laboratory of Zoonoses Cross-Border Prevention and Quarantine, Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, ChinaKey Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaYunnan Province Key Laboratory of Anti-Pathogenic Plant Resources Screening, Yunnan Province Key University Laboratory of Zoonoses Cross-Border Prevention and Quarantine, Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, ChinaYunnan Province Key Laboratory of Anti-Pathogenic Plant Resources Screening, Yunnan Province Key University Laboratory of Zoonoses Cross-Border Prevention and Quarantine, Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, ChinaKey Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaYunnan Province Key Laboratory of Anti-Pathogenic Plant Resources Screening, Yunnan Province Key University Laboratory of Zoonoses Cross-Border Prevention and Quarantine, Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, ChinaKey Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaKey Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USAYunnan Key Laboratory of Biodiversity Information, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650023, ChinaYunnan Province Key Laboratory of Anti-Pathogenic Plant Resources Screening, Yunnan Province Key University Laboratory of Zoonoses Cross-Border Prevention and Quarantine, Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, ChinaThe genus <i>Hepacivirus</i> contains single-stranded positive-sense RNA viruses belonging to the family <i>Flaviviridae</i>, which comprises 14 species. These 14 hepaciviruses have been found in different mammals, such as primates, dogs, bats, and rodents. To date, <i>Hepacivirus</i> has not been reported in the shrew genus of <i>Crocidura</i>. To study the prevalence and genetic evolution of <i>Hepacivirus</i> in small mammals in Yunnan Province, China, molecular detection of <i>Hepacivirus</i> in small mammals from Yunnan Province during 2016 and 2017 was performed using reverse-transcription polymerase chain reaction (RT-PCR). Our results showed that the overall infection rate of <i>Hepacivirus</i> in small mammals was 0.12% (2/1602), and the host animal was the Southeast Asian shrew (<i>Crocidura fuliginosa</i>) (12.5%, 2/16). Quantitative real-time PCR showed that <i>Hepacivirus</i> had the highest viral RNA copy number in the liver. Phylogenetic analysis revealed that the hepaciviruses obtained in this study does not belong to any designated species of hepaciviruses and forms an independent clade. To conclude, a novel hepacivirus was identified for the first time in <i>C. fuliginosa</i> specimens from Yunnan Province, China. This study expands the host range and viral diversity of hepaciviruses.https://www.mdpi.com/2076-0817/12/12/1400small mammalSoutheast Asian shrew<i>Crocidura fuliginosa</i><i>Flaviviridae</i><i>Hepacivirus</i> |
| spellingShingle | Ling Guo Bei Li Peiyu Han Na Dong Yan Zhu Fuli Li Haorui Si Zhengli Shi Bo Wang Xinglou Yang Yunzhi Zhang Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China Pathogens small mammal Southeast Asian shrew <i>Crocidura fuliginosa</i> <i>Flaviviridae</i> <i>Hepacivirus</i> |
| title | Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China |
| title_full | Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China |
| title_fullStr | Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China |
| title_full_unstemmed | Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China |
| title_short | Identification of a Novel Hepacivirus in Southeast Asian Shrew (<i>Crocidura fuliginosa</i>) from Yunnan Province, China |
| title_sort | identification of a novel hepacivirus in southeast asian shrew i crocidura fuliginosa i from yunnan province china |
| topic | small mammal Southeast Asian shrew <i>Crocidura fuliginosa</i> <i>Flaviviridae</i> <i>Hepacivirus</i> |
| url | https://www.mdpi.com/2076-0817/12/12/1400 |
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