Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower
Orobanche cumana Wall., sunflower broomrape, is one of the major pests for the sunflower crop. Breeding for resistant varieties in sunflower has been the most efficient method to control this parasitic weed. However, more virulent broomrape populations continuously emerge by overcoming genetic resis...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1038684/full |
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| author | Mireille Chabaud Marie-Christine Auriac Marie-Claude Boniface Sabine Delgrange Tifaine Folletti Marie-Françoise Jardinaud Alexandra Legendre Begoña Pérez-Vich Jean-Bernard Pouvreau Leonardo Velasco Philippe Delavault Stéphane Muños |
| author_facet | Mireille Chabaud Marie-Christine Auriac Marie-Claude Boniface Sabine Delgrange Tifaine Folletti Marie-Françoise Jardinaud Alexandra Legendre Begoña Pérez-Vich Jean-Bernard Pouvreau Leonardo Velasco Philippe Delavault Stéphane Muños |
| author_sort | Mireille Chabaud |
| collection | DOAJ |
| description | Orobanche cumana Wall., sunflower broomrape, is one of the major pests for the sunflower crop. Breeding for resistant varieties in sunflower has been the most efficient method to control this parasitic weed. However, more virulent broomrape populations continuously emerge by overcoming genetic resistance. It is thus essential to identify new broomrape resistances acting at various stages of the interaction and combine them to improve resistance durability. In this study, 71 wild sunflowers and wild relatives accessions from 16 Helianthus species were screened in pots for their resistance to broomrape at the late emergence stage. From this initial screen, 18 accessions from 9 species showing resistance, were phenotyped at early stages of the interaction: the induction of broomrape seed germination by sunflower root exudates, the attachment to the host root and the development of tubercles in rhizotron assays. We showed that wild Helianthus accessions are an important source of resistance to the most virulent broomrape races, affecting various stages of the interaction: the inability to induce broomrape seed germination, the development of incompatible attachments or necrotic tubercles, and the arrest of emerged structure growth. Cytological studies of incompatible attachments showed that several cellular mechanisms were shared among resistant Helianthus species. |
| first_indexed | 2024-04-11T07:22:48Z |
| format | Article |
| id | doaj.art-0da368dfbfa345489f31b49ece86d8b5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T07:22:48Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0da368dfbfa345489f31b49ece86d8b52022-12-22T04:37:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10386841038684Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflowerMireille Chabaud0Marie-Christine Auriac1Marie-Claude Boniface2Sabine Delgrange3Tifaine Folletti4Marie-Françoise Jardinaud5Alexandra Legendre6Begoña Pérez-Vich7Jean-Bernard Pouvreau8Leonardo Velasco9Philippe Delavault10Stéphane Muños11Laboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceUnité en Sciences Biologiques et Biotechnologies (US2B), Nantes Université, Centre national de la recherche scientifique (CNRS), Unité mixte de recherche 6286 (UMR 6286), Nantes, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceIAS-CSIC, Córdoba, SpainUnité en Sciences Biologiques et Biotechnologies (US2B), Nantes Université, Centre national de la recherche scientifique (CNRS), Unité mixte de recherche 6286 (UMR 6286), Nantes, FranceIAS-CSIC, Córdoba, SpainUnité en Sciences Biologiques et Biotechnologies (US2B), Nantes Université, Centre national de la recherche scientifique (CNRS), Unité mixte de recherche 6286 (UMR 6286), Nantes, FranceLaboratoire des Interactions Plantes-Microbes- Environnement (LIPME), Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Castanet-tolosan, FranceOrobanche cumana Wall., sunflower broomrape, is one of the major pests for the sunflower crop. Breeding for resistant varieties in sunflower has been the most efficient method to control this parasitic weed. However, more virulent broomrape populations continuously emerge by overcoming genetic resistance. It is thus essential to identify new broomrape resistances acting at various stages of the interaction and combine them to improve resistance durability. In this study, 71 wild sunflowers and wild relatives accessions from 16 Helianthus species were screened in pots for their resistance to broomrape at the late emergence stage. From this initial screen, 18 accessions from 9 species showing resistance, were phenotyped at early stages of the interaction: the induction of broomrape seed germination by sunflower root exudates, the attachment to the host root and the development of tubercles in rhizotron assays. We showed that wild Helianthus accessions are an important source of resistance to the most virulent broomrape races, affecting various stages of the interaction: the inability to induce broomrape seed germination, the development of incompatible attachments or necrotic tubercles, and the arrest of emerged structure growth. Cytological studies of incompatible attachments showed that several cellular mechanisms were shared among resistant Helianthus species.https://www.frontiersin.org/articles/10.3389/fpls.2022.1038684/fullHelianthusOrobanche cumanasunflowergenetic resistancephenotypingcytology |
| spellingShingle | Mireille Chabaud Marie-Christine Auriac Marie-Claude Boniface Sabine Delgrange Tifaine Folletti Marie-Françoise Jardinaud Alexandra Legendre Begoña Pérez-Vich Jean-Bernard Pouvreau Leonardo Velasco Philippe Delavault Stéphane Muños Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower Frontiers in Plant Science Helianthus Orobanche cumana sunflower genetic resistance phenotyping cytology |
| title | Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| title_full | Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| title_fullStr | Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| title_full_unstemmed | Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| title_short | Wild Helianthus species: A reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| title_sort | wild helianthus species a reservoir of resistance genes for sustainable pyramidal resistance to broomrape in sunflower |
| topic | Helianthus Orobanche cumana sunflower genetic resistance phenotyping cytology |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1038684/full |
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