Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds
In this work, freshwater microalga, Haematococcus lacustris was isolated from the River Nile, identified and deposited in genebank under name of H. lacustris isolate REH10 with accession number OK336515. N-hexane extract was produced high inhibition effects against multi-antibiotic resistant pathoge...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000510 |
| _version_ | 1811188556884869120 |
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| author | Osama M. Darwesh Rehab H. Mahmoud Sayeda M. Abdo Diaa A. Marrez |
| author_facet | Osama M. Darwesh Rehab H. Mahmoud Sayeda M. Abdo Diaa A. Marrez |
| author_sort | Osama M. Darwesh |
| collection | DOAJ |
| description | In this work, freshwater microalga, Haematococcus lacustris was isolated from the River Nile, identified and deposited in genebank under name of H. lacustris isolate REH10 with accession number OK336515. N-hexane extract was produced high inhibition effects against multi-antibiotic resistant pathogens. The n-Hexane extract was fractionated and 2 fractions (F3 & F4) exhibited high antibacterial activity (15 – 20 mm) compared with other fractions. Thus, they sub-fractionated and 2 sub-fractions produced from the F3 had high inhibition activity against all tested pathogens (18–20 mm). To identify the main compounds responsible for inhibition growth of multi-drug resistance bacteria, GC–MS chromatogram analyses was applied on the F3 and its sub-fractions 2 and 3. Five compounds detected in the 2 sub-fractions. Palmitic acid was identified as the first report antibacterial agent. The antioxidant activity of SF3–3 was reached to 86 and 80.5% for DPPH and ABTS.+ tests, respectively. |
| first_indexed | 2024-04-11T14:20:46Z |
| format | Article |
| id | doaj.art-0da73665c8364195adff50fa2ff26254 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-11T14:20:46Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-0da73665c8364195adff50fa2ff262542022-12-22T04:19:03ZengElsevierBiotechnology Reports2215-017X2022-09-0135e00753Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compoundsOsama M. Darwesh0Rehab H. Mahmoud1Sayeda M. Abdo2Diaa A. Marrez3Agricultural Microbiology Department, National Research Centre, Cairo 12622, Egypt; Corresponding author.Water Pollution Research department, National Research Centre, Cairo 12622, EgyptWater Pollution Research department, National Research Centre, Cairo 12622, EgyptFood Toxicology and Contaminants Department, National Research Centre, Cairo 12622, EgyptIn this work, freshwater microalga, Haematococcus lacustris was isolated from the River Nile, identified and deposited in genebank under name of H. lacustris isolate REH10 with accession number OK336515. N-hexane extract was produced high inhibition effects against multi-antibiotic resistant pathogens. The n-Hexane extract was fractionated and 2 fractions (F3 & F4) exhibited high antibacterial activity (15 – 20 mm) compared with other fractions. Thus, they sub-fractionated and 2 sub-fractions produced from the F3 had high inhibition activity against all tested pathogens (18–20 mm). To identify the main compounds responsible for inhibition growth of multi-drug resistance bacteria, GC–MS chromatogram analyses was applied on the F3 and its sub-fractions 2 and 3. Five compounds detected in the 2 sub-fractions. Palmitic acid was identified as the first report antibacterial agent. The antioxidant activity of SF3–3 was reached to 86 and 80.5% for DPPH and ABTS.+ tests, respectively.http://www.sciencedirect.com/science/article/pii/S2215017X22000510Haematococcus lacustrisN-Hexane extractGC–MS identificationMulti-antibiotic resistant microbesAntibacterial activity |
| spellingShingle | Osama M. Darwesh Rehab H. Mahmoud Sayeda M. Abdo Diaa A. Marrez Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds Biotechnology Reports Haematococcus lacustris N-Hexane extract GC–MS identification Multi-antibiotic resistant microbes Antibacterial activity |
| title | Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds |
| title_full | Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds |
| title_fullStr | Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds |
| title_full_unstemmed | Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds |
| title_short | Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds |
| title_sort | isolation of haematococcus lacustris as source of novel anti multi antibiotic resistant microbes agents fractionation and identification of bioactive compounds |
| topic | Haematococcus lacustris N-Hexane extract GC–MS identification Multi-antibiotic resistant microbes Antibacterial activity |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000510 |
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