The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona
Butenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intrac...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.904627/full |
| _version_ | 1811239876397367296 |
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| author | Jianli Tang Jianming Chen Yang Liu Jinjuan Hu Ziyuan Xia Xiaomin Li Haocheng He Jie Rang Yunjun Sun Ziquan Yu Jun Cui Liqiu Xia |
| author_facet | Jianli Tang Jianming Chen Yang Liu Jinjuan Hu Ziyuan Xia Xiaomin Li Haocheng He Jie Rang Yunjun Sun Ziquan Yu Jun Cui Liqiu Xia |
| author_sort | Jianli Tang |
| collection | DOAJ |
| description | Butenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intracellular phosphate levels. Nevertheless, the function of PhoU remains ambiguous in S. pogona. In this study, we investigated the effects of PhoU on the growth and the butenyl-spinosyn biosynthesis of S. pogona by constructing the mutants. Overexpression of phoU increased the production of butenyl-spinosyn to 2.2-fold that of the wild-type strain. However, the phoU deletion resulted in a severe imbalance of intracellular phosphate levels, and suppression of the growth and butenyl-spinosyn biosynthesis. Quantitative Real-time PCR (qRT-PCR) analysis, distinctive protein detection and mass spectrometry revealed that PhoU widely regulated primary metabolism, energy metabolism and DNA repair, which implied that PhoU influences the growth and butenyl-spinosyn biosynthesis of S. pogona as a global regulator. |
| first_indexed | 2024-04-12T13:08:56Z |
| format | Article |
| id | doaj.art-0da8ffd5237c4b2fa98e190216dbb795 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T13:08:56Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0da8ffd5237c4b2fa98e190216dbb7952022-12-22T03:31:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.904627904627The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogonaJianli TangJianming ChenYang LiuJinjuan HuZiyuan XiaXiaomin LiHaocheng HeJie RangYunjun SunZiquan YuJun CuiLiqiu XiaButenyl-spinosyn, a highly effective biological insecticide, is produced by Saccharopolyspora pogona. However, its application has been severely hampered by its low yield. Recent studies have shown that PhoU plays a pivotal role in regulating cell growth, secondary metabolite biosynthesis and intracellular phosphate levels. Nevertheless, the function of PhoU remains ambiguous in S. pogona. In this study, we investigated the effects of PhoU on the growth and the butenyl-spinosyn biosynthesis of S. pogona by constructing the mutants. Overexpression of phoU increased the production of butenyl-spinosyn to 2.2-fold that of the wild-type strain. However, the phoU deletion resulted in a severe imbalance of intracellular phosphate levels, and suppression of the growth and butenyl-spinosyn biosynthesis. Quantitative Real-time PCR (qRT-PCR) analysis, distinctive protein detection and mass spectrometry revealed that PhoU widely regulated primary metabolism, energy metabolism and DNA repair, which implied that PhoU influences the growth and butenyl-spinosyn biosynthesis of S. pogona as a global regulator.https://www.frontiersin.org/articles/10.3389/fmicb.2022.904627/fullPhoUbutenyl-spinosynSaccharopolyspora pogonaglobal regulatorphosphate |
| spellingShingle | Jianli Tang Jianming Chen Yang Liu Jinjuan Hu Ziyuan Xia Xiaomin Li Haocheng He Jie Rang Yunjun Sun Ziquan Yu Jun Cui Liqiu Xia The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona Frontiers in Microbiology PhoU butenyl-spinosyn Saccharopolyspora pogona global regulator phosphate |
| title | The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona |
| title_full | The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona |
| title_fullStr | The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona |
| title_full_unstemmed | The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona |
| title_short | The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona |
| title_sort | global regulator phou positively controls growth and butenyl spinosyn biosynthesis in saccharopolyspora pogona |
| topic | PhoU butenyl-spinosyn Saccharopolyspora pogona global regulator phosphate |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.904627/full |
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