Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo
Flavonoids are famous for their diverse sources, strong biological activity, and low toxicity and could be used as a natural antioxidant in animal husbandry. In this study, the purification process and antioxidant activity of flavonoids from fermented dandelion were investigated. The adsorption and...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/7/306 |
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| author | Na Yin Yuan Wang Xuerong Ren Yang Zhao Na Liu Xiaoping An Jingwei Qi |
| author_facet | Na Yin Yuan Wang Xuerong Ren Yang Zhao Na Liu Xiaoping An Jingwei Qi |
| author_sort | Na Yin |
| collection | DOAJ |
| description | Flavonoids are famous for their diverse sources, strong biological activity, and low toxicity and could be used as a natural antioxidant in animal husbandry. In this study, the purification process and antioxidant activity of flavonoids from fermented dandelion were investigated. The adsorption and desorption characterizations of AB-8 macroporous resin for flavonoids from fermented dandelion (FD) were determined and purification parameters were optimized. Qualitative analysis using UPLC-MS/MS analysis was explored to identify the components of the purified flavonoids of FD (PFDF). The antioxidant activity of PFDF in vitro and in vivo was analyzed. The optimum purification parameters were as follows: a sample concentration of 2 mg/mL, 120 mL of the sample volume, a pH of 2.0, and eluted with 90 mL of 70% ethanol (pH 5). After purification, the concentration of the flavonoids in PFDF was 356.08 mg/mL. By comparison with reference standards or the literature data, 135 kinds of flavonoids in PFDF were identified. Furthermore, PFDF had a strong reducing power and scavenging ability against 8-hydroxy radical and DPPH radical. PFDF can effectively reduce the oxidative stress of zebrafish embryos and IPCE-J2 cells by modulating antioxidant enzyme activities. In summary, the purified flavonoids from fermented dandelion have good antioxidant activity and display superior potential as a natural antioxidant in animal husbandry. |
| first_indexed | 2024-03-09T10:18:57Z |
| format | Article |
| id | doaj.art-0da92e70cbde4a91bfb9ffd1e77f423c |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T10:18:57Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-0da92e70cbde4a91bfb9ffd1e77f423c2023-12-01T22:08:14ZengMDPI AGFermentation2311-56372022-06-018730610.3390/fermentation8070306Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In VivoNa Yin0Yuan Wang1Xuerong Ren2Yang Zhao3Na Liu4Xiaoping An5Jingwei Qi6College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, ChinaFlavonoids are famous for their diverse sources, strong biological activity, and low toxicity and could be used as a natural antioxidant in animal husbandry. In this study, the purification process and antioxidant activity of flavonoids from fermented dandelion were investigated. The adsorption and desorption characterizations of AB-8 macroporous resin for flavonoids from fermented dandelion (FD) were determined and purification parameters were optimized. Qualitative analysis using UPLC-MS/MS analysis was explored to identify the components of the purified flavonoids of FD (PFDF). The antioxidant activity of PFDF in vitro and in vivo was analyzed. The optimum purification parameters were as follows: a sample concentration of 2 mg/mL, 120 mL of the sample volume, a pH of 2.0, and eluted with 90 mL of 70% ethanol (pH 5). After purification, the concentration of the flavonoids in PFDF was 356.08 mg/mL. By comparison with reference standards or the literature data, 135 kinds of flavonoids in PFDF were identified. Furthermore, PFDF had a strong reducing power and scavenging ability against 8-hydroxy radical and DPPH radical. PFDF can effectively reduce the oxidative stress of zebrafish embryos and IPCE-J2 cells by modulating antioxidant enzyme activities. In summary, the purified flavonoids from fermented dandelion have good antioxidant activity and display superior potential as a natural antioxidant in animal husbandry.https://www.mdpi.com/2311-5637/8/7/306fermented dandelionflavonoidspurificationcharacterizationantioxidant activity |
| spellingShingle | Na Yin Yuan Wang Xuerong Ren Yang Zhao Na Liu Xiaoping An Jingwei Qi Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo Fermentation fermented dandelion flavonoids purification characterization antioxidant activity |
| title | Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo |
| title_full | Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo |
| title_fullStr | Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo |
| title_full_unstemmed | Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo |
| title_short | Isolation and Characterization of Flavonoids from Fermented Dandelion (<i>Taraxacum mongolicum</i> Hand.-Mazz.), and Assessment of Its Antioxidant Actions In Vitro and In Vivo |
| title_sort | isolation and characterization of flavonoids from fermented dandelion i taraxacum mongolicum i hand mazz and assessment of its antioxidant actions in vitro and in vivo |
| topic | fermented dandelion flavonoids purification characterization antioxidant activity |
| url | https://www.mdpi.com/2311-5637/8/7/306 |
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