Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin
Background and Aim: Asthma, a chronic disease affecting humans and animals, has recently become increasingly prevalent and steadily widespread. The alternative treatment of asthma using helminth infections or helminth-derived immunomodulatory molecules (IMs) has been evaluated and demonstrated signi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-11-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/November-2023/24.pdf |
| _version_ | 1797449538909241344 |
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| author | Nipa Thammasonthijarern Kobporn Boonnak Onrapak Reamtong Thanyaluk Krasae Janyaporn Thankansakul Wallaya Phongphaew Sumate Ampawong Poom Adisakwattana |
| author_facet | Nipa Thammasonthijarern Kobporn Boonnak Onrapak Reamtong Thanyaluk Krasae Janyaporn Thankansakul Wallaya Phongphaew Sumate Ampawong Poom Adisakwattana |
| author_sort | Nipa Thammasonthijarern |
| collection | DOAJ |
| description | Background and Aim: Asthma, a chronic disease affecting humans and animals, has recently become increasingly prevalent and steadily widespread. The alternative treatment of asthma using helminth infections or helminth-derived immunomodulatory molecules (IMs) has been evaluated and demonstrated significant amelioration of disease severity index in vitro and in vivo. Trichinella spiralis, a parasitic nematode and its IMs, elicits a potential to relieve asthma and other immune-related disorders. In this study, we investigated the immunomodulatory function of recombinant T. spiralis novel cystatin (rTsCstN) in ameliorating acute inflammatory asthma disorders in a murine model.
Materials and Methods: Female BALB/c mice were sensitized using intraperitoneal injection of ovalbumin (OVA)/alum and subsequently challenged with intranasal administration of OVA alone or OVA + rTsCstN for 3 consecutive days, producing OVA-induced allergic asthma models. To evaluate the therapeutic efficacy of rTsCstN, the inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) and OVA-specific immunoglobulin E levels in serum were assessed. Histological alterations in the lung tissues were determined by hematoxylin and eosin (H&E) staining and eventually scored for the extent of inflammatory cell infiltration.
Results: The asthmatic mouse models challenged with OVA + rTsCstN demonstrated a significant reduction of eosinophils (p < 0.01), macrophages (p < 0.05), and cytokines tumor necrosis factor-α (p < 0.05) and interferon (IFN)-γ (p < 0.05) in BALF when compared with the mice challenged with OVA alone. However, the levels of interleukin (IL)-4 and IL-10 remained unchanged. Histological examination revealed that mice administered OVA + rTsCstN were less likely to have inflammatory cell infiltration in their perivascular and peribronchial lung tissues than those administered OVA alone.
Conclusion: Recombinant T. spiralis novel cystatin demonstrated immunomodulatory effects to reduce severe pathogenic alterations in asthma mouse models, encouraging a viable alternative treatment for asthma and other immunoregulatory disorders in humans and animals in the future. |
| first_indexed | 2024-03-09T14:26:26Z |
| format | Article |
| id | doaj.art-0da938e2b22e4f098825969d10022e68 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-09T14:26:26Z |
| publishDate | 2023-11-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-0da938e2b22e4f098825969d10022e682023-11-28T07:05:06ZengVeterinary WorldVeterinary World0972-89882231-09162023-11-0116112366237310.14202/vetworld.2023.2366-2373Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatinNipa Thammasonthijarern0https://orcid.org/0000-0003-0093-1545Kobporn Boonnak1https://orcid.org/0000-0002-7536-8964Onrapak Reamtong2https://orcid.org/0000-0002-1154-6485Thanyaluk Krasae3Janyaporn Thankansakul4Wallaya Phongphaew5https://orcid.org/0000-0002-6618-9833Sumate Ampawong6https://orcid.org/0000-0002-5378-2996Poom Adisakwattana7https://orcid.org/0000-0002-0313-9900Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Laboratory Animal Science Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Background and Aim: Asthma, a chronic disease affecting humans and animals, has recently become increasingly prevalent and steadily widespread. The alternative treatment of asthma using helminth infections or helminth-derived immunomodulatory molecules (IMs) has been evaluated and demonstrated significant amelioration of disease severity index in vitro and in vivo. Trichinella spiralis, a parasitic nematode and its IMs, elicits a potential to relieve asthma and other immune-related disorders. In this study, we investigated the immunomodulatory function of recombinant T. spiralis novel cystatin (rTsCstN) in ameliorating acute inflammatory asthma disorders in a murine model. Materials and Methods: Female BALB/c mice were sensitized using intraperitoneal injection of ovalbumin (OVA)/alum and subsequently challenged with intranasal administration of OVA alone or OVA + rTsCstN for 3 consecutive days, producing OVA-induced allergic asthma models. To evaluate the therapeutic efficacy of rTsCstN, the inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) and OVA-specific immunoglobulin E levels in serum were assessed. Histological alterations in the lung tissues were determined by hematoxylin and eosin (H&E) staining and eventually scored for the extent of inflammatory cell infiltration. Results: The asthmatic mouse models challenged with OVA + rTsCstN demonstrated a significant reduction of eosinophils (p < 0.01), macrophages (p < 0.05), and cytokines tumor necrosis factor-α (p < 0.05) and interferon (IFN)-γ (p < 0.05) in BALF when compared with the mice challenged with OVA alone. However, the levels of interleukin (IL)-4 and IL-10 remained unchanged. Histological examination revealed that mice administered OVA + rTsCstN were less likely to have inflammatory cell infiltration in their perivascular and peribronchial lung tissues than those administered OVA alone. Conclusion: Recombinant T. spiralis novel cystatin demonstrated immunomodulatory effects to reduce severe pathogenic alterations in asthma mouse models, encouraging a viable alternative treatment for asthma and other immunoregulatory disorders in humans and animals in the future.https://www.veterinaryworld.org/Vol.16/November-2023/24.pdfasthmaimmunomodulatory moleculerecombinant trichinella spiralis novel cystatintrichinella spiralis |
| spellingShingle | Nipa Thammasonthijarern Kobporn Boonnak Onrapak Reamtong Thanyaluk Krasae Janyaporn Thankansakul Wallaya Phongphaew Sumate Ampawong Poom Adisakwattana Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin Veterinary World asthma immunomodulatory molecule recombinant trichinella spiralis novel cystatin trichinella spiralis |
| title | Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin |
| title_full | Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin |
| title_fullStr | Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin |
| title_full_unstemmed | Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin |
| title_short | Amelioration of ovalbumin-induced lung inflammation in a mouse model by Trichinella spiralis novel cystatin |
| title_sort | amelioration of ovalbumin induced lung inflammation in a mouse model by trichinella spiralis novel cystatin |
| topic | asthma immunomodulatory molecule recombinant trichinella spiralis novel cystatin trichinella spiralis |
| url | https://www.veterinaryworld.org/Vol.16/November-2023/24.pdf |
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