Real-time dynamic imaging of virus distribution in vivo.
The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-02-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3039657?pdf=render |
| _version_ | 1818499085806600192 |
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| author | Sean E Hofherr Kristen E Adams Christopher Y Chen Shannon May Eric A Weaver Michael A Barry |
| author_facet | Sean E Hofherr Kristen E Adams Christopher Y Chen Shannon May Eric A Weaver Michael A Barry |
| author_sort | Sean E Hofherr |
| collection | DOAJ |
| description | The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with injection, appearing in the heart within 500 milliseconds, distributing in the bloodstream and throughout the animal within 7 seconds, and that the bulk of virus distribution was essentially complete within 3 minutes. These data provide the first in vivo real-time tracking of the rapid initial events of systemic virus infection. |
| first_indexed | 2024-12-10T20:24:27Z |
| format | Article |
| id | doaj.art-0da95cd4a0fd4a75844982b7fac02ee7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T20:24:27Z |
| publishDate | 2011-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0da95cd4a0fd4a75844982b7fac02ee72022-12-22T01:34:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-02-0162e1707610.1371/journal.pone.0017076Real-time dynamic imaging of virus distribution in vivo.Sean E HofherrKristen E AdamsChristopher Y ChenShannon MayEric A WeaverMichael A BarryThe distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with injection, appearing in the heart within 500 milliseconds, distributing in the bloodstream and throughout the animal within 7 seconds, and that the bulk of virus distribution was essentially complete within 3 minutes. These data provide the first in vivo real-time tracking of the rapid initial events of systemic virus infection.http://europepmc.org/articles/PMC3039657?pdf=render |
| spellingShingle | Sean E Hofherr Kristen E Adams Christopher Y Chen Shannon May Eric A Weaver Michael A Barry Real-time dynamic imaging of virus distribution in vivo. PLoS ONE |
| title | Real-time dynamic imaging of virus distribution in vivo. |
| title_full | Real-time dynamic imaging of virus distribution in vivo. |
| title_fullStr | Real-time dynamic imaging of virus distribution in vivo. |
| title_full_unstemmed | Real-time dynamic imaging of virus distribution in vivo. |
| title_short | Real-time dynamic imaging of virus distribution in vivo. |
| title_sort | real time dynamic imaging of virus distribution in vivo |
| url | http://europepmc.org/articles/PMC3039657?pdf=render |
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