Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System
This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cel...
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| Format: | Article |
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MDPI AG
2015-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/2/2763 |
| _version_ | 1818015414365454336 |
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| author | Yang Zhao Deyong Chen Yana Luo Feng Chen Xiaoting Zhao Mei Jiang Wentao Yue Rong Long Junbo Wang Jian Chen |
| author_facet | Yang Zhao Deyong Chen Yana Luo Feng Chen Xiaoting Zhao Mei Jiang Wentao Yue Rong Long Junbo Wang Jian Chen |
| author_sort | Yang Zhao |
| collection | DOAJ |
| description | This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties. |
| first_indexed | 2024-04-14T06:56:45Z |
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| id | doaj.art-0daae0c880c94f5daf1669e7d2033da6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T06:56:45Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-0daae0c880c94f5daf1669e7d2033da62022-12-22T02:06:51ZengMDPI AGSensors1424-82202015-01-011522763277310.3390/s150202763s150202763Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic SystemYang Zhao0Deyong Chen1Yana Luo2Feng Chen3Xiaoting Zhao4Mei Jiang5Wentao Yue6Rong Long7Junbo Wang8Jian Chen9State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaDepartment of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, ChinaDepartment of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, ChinaDepartment of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, ChinaDepartment of Mechanical Engineering, University of Alberta, Edmonton, AB T6G 2G8, CanadaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, ChinaThis paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties.http://www.mdpi.com/1424-8220/15/2/2763microfluidicssingle-cell analysiscellular biophysicsinstantaneous Young’s modulusspecific membrane capacitance |
| spellingShingle | Yang Zhao Deyong Chen Yana Luo Feng Chen Xiaoting Zhao Mei Jiang Wentao Yue Rong Long Junbo Wang Jian Chen Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System Sensors microfluidics single-cell analysis cellular biophysics instantaneous Young’s modulus specific membrane capacitance |
| title | Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System |
| title_full | Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System |
| title_fullStr | Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System |
| title_full_unstemmed | Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System |
| title_short | Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System |
| title_sort | simultaneous characterization of instantaneous young s modulus and specific membrane capacitance of single cells using a microfluidic system |
| topic | microfluidics single-cell analysis cellular biophysics instantaneous Young’s modulus specific membrane capacitance |
| url | http://www.mdpi.com/1424-8220/15/2/2763 |
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