Impact of DNA purification method and primer selection on 16S rRNA gene metabarcoding on wine

Impact of DNA purification method and primer selection on 16S rRNA gene metabarcoding on wine

Aim: The high-throughput sequencing methods have revolutionized the study of the microbiota in different matrices including those of the grapevine production chain. DNA extraction is a crucial step in the sample processing. In this study, we compared different DNA purification methods and two primer...

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Bibliographic Details
Main Authors: Francesco Cerutti, Diego Cravero, Antonella Costantini, Laura Pulcini, Paola Modesto, Pier Luigi Acutis, Enrico Vaudano, Simone Peletto
Format: Article
Language:English
Published: International Viticulture and Enology Society 2019-08-01
Series:OENO One
Subjects:
wine microbiota
wine DNA purification
16S rRNA gene metabarcoding
wine NGS
wine microbial community
Online Access:https://oeno-one.eu/article/view/2368

Internet

https://oeno-one.eu/article/view/2368

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