Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging
Hendrik Reynders,1 Indra Van Zundert,1 Rui Silva,1– 3 Bram Carlier,3 Olivier Deschaume,4 Carmen Bartic,4 Susana Rocha,1 Sergey Basov,5 Margriet J Van Bael,5 Uwe Himmelreich,3 Thierry Verbiest,1 Ana Zamora1,3 1Molecular Imaging and Photonics, KU Leuven, Leuven, Belgium; 2Engineering Department, Oport...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-12-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/label-free-iron-oxide-nanoparticles-as-multimodal-contrast-agents-in-c-peer-reviewed-fulltext-article-IJN |
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| author | Reynders H Van Zundert I Silva R Carlier B Deschaume O Bartic C Rocha S Basov S Van Bael MJ Himmelreich U Verbiest T Zamora A |
| author_facet | Reynders H Van Zundert I Silva R Carlier B Deschaume O Bartic C Rocha S Basov S Van Bael MJ Himmelreich U Verbiest T Zamora A |
| author_sort | Reynders H |
| collection | DOAJ |
| description | Hendrik Reynders,1 Indra Van Zundert,1 Rui Silva,1– 3 Bram Carlier,3 Olivier Deschaume,4 Carmen Bartic,4 Susana Rocha,1 Sergey Basov,5 Margriet J Van Bael,5 Uwe Himmelreich,3 Thierry Verbiest,1 Ana Zamora1,3 1Molecular Imaging and Photonics, KU Leuven, Leuven, Belgium; 2Engineering Department, Oporto University, Porto, Portugal; 3Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium; 4Laboratory for Soft Matter and Biophysics, KU Leuven, Leuven, Belgium; 5Quantum Solid State Physics, KU Leuven, Leuven, BelgiumCorrespondence: Ana Zamora Email anamaria.zamoramartinez@kuleuven.beIntroduction: The inherent fluorescence properties of iron oxide nanoparticles (IONPs) were characterized, and their applicability for multiphoton imaging in cells was tested in combination with their magnetic resonance imaging (MRI) capabilities.Methods: Superparamagnetic iron oxide nanoparticles were synthesized and subsequently coated with polyethylene glycol to make them water-dispersible. Further characterization of the particles was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), dynamic light scattering (DLS), superconducting quantum interference device (SQUID) and magnetic resonance relaxivity measurements. MRI and fluorescence properties of bare IONPs were first studied in solution and subsequently in A549-labeled cells.Results: The particles, with a core size of 11.3 ± 4.5 nm, showed a good negative MRI contrast in tissue-mimicking phantoms. In vitro studies in mammalian A549 cells demonstrate that these IONPs are biocompatible and can also produce significant T2/T2* contrast enhancement in IONPs-labeled cells. Furthermore, excitation-wavelength dependent photoluminescence was observed under one- and two-photon excitation.Discussion: The obtained results indicated that IONPs could be used for fluorescence label-free bioimaging at multiple wavelengths, which was proven by multiphoton imaging of IONPs internalization in A549 cancer cells.Keywords: iron oxide nanoparticles, photoluminescence, biomedical imaging, MRI, cancer cells, multimodal imaging |
| first_indexed | 2024-12-22T00:39:18Z |
| format | Article |
| id | doaj.art-0dadc4f760d64dd48ffeef255cfcc79c |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-22T00:39:18Z |
| publishDate | 2021-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-0dadc4f760d64dd48ffeef255cfcc79c2022-12-21T18:44:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132021-12-01Volume 168375838971922Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance ImagingReynders HVan Zundert ISilva RCarlier BDeschaume OBartic CRocha SBasov SVan Bael MJHimmelreich UVerbiest TZamora AHendrik Reynders,1 Indra Van Zundert,1 Rui Silva,1– 3 Bram Carlier,3 Olivier Deschaume,4 Carmen Bartic,4 Susana Rocha,1 Sergey Basov,5 Margriet J Van Bael,5 Uwe Himmelreich,3 Thierry Verbiest,1 Ana Zamora1,3 1Molecular Imaging and Photonics, KU Leuven, Leuven, Belgium; 2Engineering Department, Oporto University, Porto, Portugal; 3Biomedical MRI, Department of Imaging and Pathology, KU Leuven, Leuven, Belgium; 4Laboratory for Soft Matter and Biophysics, KU Leuven, Leuven, Belgium; 5Quantum Solid State Physics, KU Leuven, Leuven, BelgiumCorrespondence: Ana Zamora Email anamaria.zamoramartinez@kuleuven.beIntroduction: The inherent fluorescence properties of iron oxide nanoparticles (IONPs) were characterized, and their applicability for multiphoton imaging in cells was tested in combination with their magnetic resonance imaging (MRI) capabilities.Methods: Superparamagnetic iron oxide nanoparticles were synthesized and subsequently coated with polyethylene glycol to make them water-dispersible. Further characterization of the particles was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), dynamic light scattering (DLS), superconducting quantum interference device (SQUID) and magnetic resonance relaxivity measurements. MRI and fluorescence properties of bare IONPs were first studied in solution and subsequently in A549-labeled cells.Results: The particles, with a core size of 11.3 ± 4.5 nm, showed a good negative MRI contrast in tissue-mimicking phantoms. In vitro studies in mammalian A549 cells demonstrate that these IONPs are biocompatible and can also produce significant T2/T2* contrast enhancement in IONPs-labeled cells. Furthermore, excitation-wavelength dependent photoluminescence was observed under one- and two-photon excitation.Discussion: The obtained results indicated that IONPs could be used for fluorescence label-free bioimaging at multiple wavelengths, which was proven by multiphoton imaging of IONPs internalization in A549 cancer cells.Keywords: iron oxide nanoparticles, photoluminescence, biomedical imaging, MRI, cancer cells, multimodal imaginghttps://www.dovepress.com/label-free-iron-oxide-nanoparticles-as-multimodal-contrast-agents-in-c-peer-reviewed-fulltext-article-IJNiron oxide nanoparticlesphotoluminescencebiomedical imagingmricancer cellsmultimodal imaging |
| spellingShingle | Reynders H Van Zundert I Silva R Carlier B Deschaume O Bartic C Rocha S Basov S Van Bael MJ Himmelreich U Verbiest T Zamora A Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging International Journal of Nanomedicine iron oxide nanoparticles photoluminescence biomedical imaging mri cancer cells multimodal imaging |
| title | Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging |
| title_full | Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging |
| title_fullStr | Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging |
| title_full_unstemmed | Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging |
| title_short | Label-Free Iron Oxide Nanoparticles as Multimodal Contrast Agents in Cells Using Multi-Photon and Magnetic Resonance Imaging |
| title_sort | label free iron oxide nanoparticles as multimodal contrast agents in cells using multi photon and magnetic resonance imaging |
| topic | iron oxide nanoparticles photoluminescence biomedical imaging mri cancer cells multimodal imaging |
| url | https://www.dovepress.com/label-free-iron-oxide-nanoparticles-as-multimodal-contrast-agents-in-c-peer-reviewed-fulltext-article-IJN |
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