Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway
Abstract Background Staphylococcus aureus (S. aureus) infection accounts for more than 50% of the osteomyelitis cases. Currently, methicillin-resistant S. aureus (MRSA) strains present an urgent medical problem. The YycFG two-component regulatory system (TCS) can allow bacteria to rapidly adapt to p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-1055-z |
| _version_ | 1798039490323808256 |
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| author | Shizhou Wu Fuguo Huang Hui Zhang Lei Lei |
| author_facet | Shizhou Wu Fuguo Huang Hui Zhang Lei Lei |
| author_sort | Shizhou Wu |
| collection | DOAJ |
| description | Abstract Background Staphylococcus aureus (S. aureus) infection accounts for more than 50% of the osteomyelitis cases. Currently, methicillin-resistant S. aureus (MRSA) strains present an urgent medical problem. The YycFG two-component regulatory system (TCS) can allow bacteria to rapidly adapt to physical, chemical, and biological stresses. To define the role of YycFG in modulation virulence of S. aureus in osteomyelitis, we isolated clinical MRSA strains and compared these with ATCC29213 methicillin-sensitive S. aureus (MSSA). Methods In the present study, 13 MRSA strains from chronic osteomyelitis tissues were isolated. The in-depth sequencing of 16S rRNA amplicons of the samples was conducted. Bacterial growth was monitored, and biofilm biomass was determined by crystal violet microtiter assay. Furthermore, quantitative RT-PCR analysis was adopted to identify the expression of yycF/G/H and icaA/D in MRSA and MSSA strains. Analysis of variance with one-way ANOVA was used for statistical analysis. Results The in-depth sequencing of 16S rRNA amplicons of the clinical samples indicated a polymicrobial infection, with the phylum Firmicutes made up 13% of the microbial population. The MRSA strains showed an accelerated growth rate compared to the MSSA strains. Of note, MRSA biofilms showed an accumulation of an intercellular polysaccharides matrix and enhanced biomass upon microscopic examination. Furthermore, MRSA strains had a higher expression of the yycF/G/H and icaA/D genes and adhesion force. Conclusions These data suggested the roles of intercellular polysaccharide in S. aureus pathogenesis, indicating a possible association between YycFG pathways and MRSA strain virulence. |
| first_indexed | 2024-04-11T21:54:36Z |
| format | Article |
| id | doaj.art-0daf4f1cd3334e50808320ac482fbf99 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T21:54:36Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-0daf4f1cd3334e50808320ac482fbf992022-12-22T04:01:09ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2019-01-011411810.1186/s13018-018-1055-zStaphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathwayShizhou Wu0Fuguo Huang1Hui Zhang2Lei Lei3Department of Orthopedics, West China Hospital , Sichuan UniversityDepartment of Orthopedics, West China Hospital , Sichuan UniversityDepartment of Orthopedics, West China Hospital , Sichuan UniversityState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Disease, Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan UniversityAbstract Background Staphylococcus aureus (S. aureus) infection accounts for more than 50% of the osteomyelitis cases. Currently, methicillin-resistant S. aureus (MRSA) strains present an urgent medical problem. The YycFG two-component regulatory system (TCS) can allow bacteria to rapidly adapt to physical, chemical, and biological stresses. To define the role of YycFG in modulation virulence of S. aureus in osteomyelitis, we isolated clinical MRSA strains and compared these with ATCC29213 methicillin-sensitive S. aureus (MSSA). Methods In the present study, 13 MRSA strains from chronic osteomyelitis tissues were isolated. The in-depth sequencing of 16S rRNA amplicons of the samples was conducted. Bacterial growth was monitored, and biofilm biomass was determined by crystal violet microtiter assay. Furthermore, quantitative RT-PCR analysis was adopted to identify the expression of yycF/G/H and icaA/D in MRSA and MSSA strains. Analysis of variance with one-way ANOVA was used for statistical analysis. Results The in-depth sequencing of 16S rRNA amplicons of the clinical samples indicated a polymicrobial infection, with the phylum Firmicutes made up 13% of the microbial population. The MRSA strains showed an accelerated growth rate compared to the MSSA strains. Of note, MRSA biofilms showed an accumulation of an intercellular polysaccharides matrix and enhanced biomass upon microscopic examination. Furthermore, MRSA strains had a higher expression of the yycF/G/H and icaA/D genes and adhesion force. Conclusions These data suggested the roles of intercellular polysaccharide in S. aureus pathogenesis, indicating a possible association between YycFG pathways and MRSA strain virulence.http://link.springer.com/article/10.1186/s13018-018-1055-zOsteomyelitisStaphylococcus aureusTwo-component regulatory systemMRSABiofilm |
| spellingShingle | Shizhou Wu Fuguo Huang Hui Zhang Lei Lei Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway Journal of Orthopaedic Surgery and Research Osteomyelitis Staphylococcus aureus Two-component regulatory system MRSA Biofilm |
| title | Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway |
| title_full | Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway |
| title_fullStr | Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway |
| title_full_unstemmed | Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway |
| title_short | Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway |
| title_sort | staphylococcus aureus biofilm organization modulated by yycfg two component regulatory pathway |
| topic | Osteomyelitis Staphylococcus aureus Two-component regulatory system MRSA Biofilm |
| url | http://link.springer.com/article/10.1186/s13018-018-1055-z |
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