Transcriptome analysis of PK-15 cells expressing CSFV NS4A
Abstract Background Classical swine fever (CSF) is a severe disease of pigs that results in huge economic losses worldwide and is caused by classical swine fever virus (CSFV). CSFV nonstructural protein 4 A (NS4A) plays a crucial role in infectious CSFV particle formation. However, the function of N...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03533-9 |
| _version_ | 1797980331851120640 |
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| author | Huifang Lv Zhifeng Peng Bingxin Jia Huiyuan Jing Sufang Cao Zhikun Xu Wang Dong |
| author_facet | Huifang Lv Zhifeng Peng Bingxin Jia Huiyuan Jing Sufang Cao Zhikun Xu Wang Dong |
| author_sort | Huifang Lv |
| collection | DOAJ |
| description | Abstract Background Classical swine fever (CSF) is a severe disease of pigs that results in huge economic losses worldwide and is caused by classical swine fever virus (CSFV). CSFV nonstructural protein 4 A (NS4A) plays a crucial role in infectious CSFV particle formation. However, the function of NS4A during CSFV infection is not well understood. Results In this study, we used RNA-seq to investigate the functional role of CSFV NS4A in PK-15 cells. A total of 3893 differentially expressed genes (DEGs) were identified in PK-15 cells expressing NS4A compared to cells expressing the empty vector (NC). Twelve DEGs were selected and further verified by RT‒qPCR. GO and KEGG enrichment analyses revealed that these DEGs were associated with multiple biological functions, including cell adhesion, apoptosis, host defence response, the inflammatory response, the immune response, and autophagy. Interestingly, some genes associated with host immune defence and inflammatory response were downregulated, and some genes associated with host apoptosis and autophagy were upregulated. Conclusion CSFV NS4A inhibits the innate immune response, and suppresses the expression of important genes associated with defence response to viruses and inflammatory response, and regulates cell adhesion, apoptosis and autophagy. |
| first_indexed | 2024-04-11T05:52:49Z |
| format | Article |
| id | doaj.art-0db14f69c1bb4a9ca277b64078a921a5 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T05:52:49Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-0db14f69c1bb4a9ca277b64078a921a52022-12-22T04:42:00ZengBMCBMC Veterinary Research1746-61482022-12-0118111010.1186/s12917-022-03533-9Transcriptome analysis of PK-15 cells expressing CSFV NS4AHuifang Lv0Zhifeng Peng1Bingxin Jia2Huiyuan Jing3Sufang Cao4Zhikun Xu5Wang Dong6Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and EconomyAbstract Background Classical swine fever (CSF) is a severe disease of pigs that results in huge economic losses worldwide and is caused by classical swine fever virus (CSFV). CSFV nonstructural protein 4 A (NS4A) plays a crucial role in infectious CSFV particle formation. However, the function of NS4A during CSFV infection is not well understood. Results In this study, we used RNA-seq to investigate the functional role of CSFV NS4A in PK-15 cells. A total of 3893 differentially expressed genes (DEGs) were identified in PK-15 cells expressing NS4A compared to cells expressing the empty vector (NC). Twelve DEGs were selected and further verified by RT‒qPCR. GO and KEGG enrichment analyses revealed that these DEGs were associated with multiple biological functions, including cell adhesion, apoptosis, host defence response, the inflammatory response, the immune response, and autophagy. Interestingly, some genes associated with host immune defence and inflammatory response were downregulated, and some genes associated with host apoptosis and autophagy were upregulated. Conclusion CSFV NS4A inhibits the innate immune response, and suppresses the expression of important genes associated with defence response to viruses and inflammatory response, and regulates cell adhesion, apoptosis and autophagy.https://doi.org/10.1186/s12917-022-03533-9RNA-seqClassical swine fever virusNS4AVirus replicationImmune defence |
| spellingShingle | Huifang Lv Zhifeng Peng Bingxin Jia Huiyuan Jing Sufang Cao Zhikun Xu Wang Dong Transcriptome analysis of PK-15 cells expressing CSFV NS4A BMC Veterinary Research RNA-seq Classical swine fever virus NS4A Virus replication Immune defence |
| title | Transcriptome analysis of PK-15 cells expressing CSFV NS4A |
| title_full | Transcriptome analysis of PK-15 cells expressing CSFV NS4A |
| title_fullStr | Transcriptome analysis of PK-15 cells expressing CSFV NS4A |
| title_full_unstemmed | Transcriptome analysis of PK-15 cells expressing CSFV NS4A |
| title_short | Transcriptome analysis of PK-15 cells expressing CSFV NS4A |
| title_sort | transcriptome analysis of pk 15 cells expressing csfv ns4a |
| topic | RNA-seq Classical swine fever virus NS4A Virus replication Immune defence |
| url | https://doi.org/10.1186/s12917-022-03533-9 |
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