Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas
The production of renewable energy or biochemicals is gaining more attention to minimize the emissions of greenhouse gases such as methane (CH<sub>4</sub>) and carbon dioxide for sustainable development. In the present study, the influence of copper (Cu)- and iron (Fe)-based nanoparticle...
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MDPI AG
2023-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/18/3667 |
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| author | Sanjay K. S. Patel Rahul K. Gupta In-Won Kim Jung-Kul Lee |
| author_facet | Sanjay K. S. Patel Rahul K. Gupta In-Won Kim Jung-Kul Lee |
| author_sort | Sanjay K. S. Patel |
| collection | DOAJ |
| description | The production of renewable energy or biochemicals is gaining more attention to minimize the emissions of greenhouse gases such as methane (CH<sub>4</sub>) and carbon dioxide for sustainable development. In the present study, the influence of copper (Cu)- and iron (Fe)-based nanoparticles (NPs), such as Cu, Fe<sub>3</sub>O<sub>4</sub>, and CuFe<sub>2</sub>O<sub>4</sub>, was evaluated during the growth of methanotrophs for inoculum preparation and on the development of a polymeric-matrix-based encapsulation system to enhance methanol production from simulated biogas (CH<sub>4</sub> and CO<sub>2</sub>). The use of simulated biogas feed and the presence of NP-derived inoculums produce a remarkable enhancement in methanol production up to 149% and 167% for <i>Methyloferula stellata</i> and <i>Methylocystis bryophila</i> free-cells-based bioconversion, respectively, compared with the use of pure CH<sub>4</sub> as a control feed during the growth stage. Furthermore, these methanotrophs encapsulated within a polymeric matrix and NPs-based systems exhibited high methanol production of up to 156%, with a maximum methanol accumulation of 12.8 mmol/L over free cells. Furthermore, after encapsulation, the methanotrophs improved the stability of residual methanol production and retained up to 62.5-fold higher production potential than free cells under repeated batch reusability of 10 cycles. In the presence of CH<sub>4</sub> vectors, methanol production by <i>M. bryophila</i> improved up to 16.4 mmol/L and retained 20% higher recycling stability for methanol production in paraffin oil. These findings suggest that Cu and Fe NPs can be beneficially employed with a polymeric matrix to encapsulate methanotrophs and improve methanol production. |
| first_indexed | 2024-03-10T22:12:00Z |
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| id | doaj.art-0db6ab97f1804d338a43dd3d56303a46 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T22:12:00Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | Polymers |
| spelling | doaj.art-0db6ab97f1804d338a43dd3d56303a462023-11-19T12:34:06ZengMDPI AGPolymers2073-43602023-09-011518366710.3390/polym15183667Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated BiogasSanjay K. S. Patel0Rahul K. Gupta1In-Won Kim2Jung-Kul Lee3Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of KoreaThe production of renewable energy or biochemicals is gaining more attention to minimize the emissions of greenhouse gases such as methane (CH<sub>4</sub>) and carbon dioxide for sustainable development. In the present study, the influence of copper (Cu)- and iron (Fe)-based nanoparticles (NPs), such as Cu, Fe<sub>3</sub>O<sub>4</sub>, and CuFe<sub>2</sub>O<sub>4</sub>, was evaluated during the growth of methanotrophs for inoculum preparation and on the development of a polymeric-matrix-based encapsulation system to enhance methanol production from simulated biogas (CH<sub>4</sub> and CO<sub>2</sub>). The use of simulated biogas feed and the presence of NP-derived inoculums produce a remarkable enhancement in methanol production up to 149% and 167% for <i>Methyloferula stellata</i> and <i>Methylocystis bryophila</i> free-cells-based bioconversion, respectively, compared with the use of pure CH<sub>4</sub> as a control feed during the growth stage. Furthermore, these methanotrophs encapsulated within a polymeric matrix and NPs-based systems exhibited high methanol production of up to 156%, with a maximum methanol accumulation of 12.8 mmol/L over free cells. Furthermore, after encapsulation, the methanotrophs improved the stability of residual methanol production and retained up to 62.5-fold higher production potential than free cells under repeated batch reusability of 10 cycles. In the presence of CH<sub>4</sub> vectors, methanol production by <i>M. bryophila</i> improved up to 16.4 mmol/L and retained 20% higher recycling stability for methanol production in paraffin oil. These findings suggest that Cu and Fe NPs can be beneficially employed with a polymeric matrix to encapsulate methanotrophs and improve methanol production.https://www.mdpi.com/2073-4360/15/18/3667biogasgreenhouse gasesencapsulationmethanolmethanotrophspolymeric matrix |
| spellingShingle | Sanjay K. S. Patel Rahul K. Gupta In-Won Kim Jung-Kul Lee Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas Polymers biogas greenhouse gases encapsulation methanol methanotrophs polymeric matrix |
| title | Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas |
| title_full | Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas |
| title_fullStr | Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas |
| title_full_unstemmed | Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas |
| title_short | Encapsulation of Methanotrophs within a Polymeric Matrix Containing Copper- and Iron-Based Nanoparticles to Enhance Methanol Production from a Simulated Biogas |
| title_sort | encapsulation of methanotrophs within a polymeric matrix containing copper and iron based nanoparticles to enhance methanol production from a simulated biogas |
| topic | biogas greenhouse gases encapsulation methanol methanotrophs polymeric matrix |
| url | https://www.mdpi.com/2073-4360/15/18/3667 |
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