The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study
Abstract Background This is a phase II clinical trial to investigate the immunotherapeutic effect of Curcumin, Piperine, and Taurine (CPT) combination in hepatocellular carcinoma (HCC). Methods Twenty-six HCC patients aged (50–80 years) were recruited for administration of a daily dose of 5 g of cur...
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| Format: | Article |
| Language: | English |
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Springer
2023-09-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-023-00785-1 |
| _version_ | 1797452146803736576 |
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| author | Raghda R. kotb Ahmed M. Afifi Motawa E. EL-Houseini Mohamed Ezz-Elarab Emad B. Basalious Mervat M. Omran Mona S. Abdellateif |
| author_facet | Raghda R. kotb Ahmed M. Afifi Motawa E. EL-Houseini Mohamed Ezz-Elarab Emad B. Basalious Mervat M. Omran Mona S. Abdellateif |
| author_sort | Raghda R. kotb |
| collection | DOAJ |
| description | Abstract Background This is a phase II clinical trial to investigate the immunotherapeutic effect of Curcumin, Piperine, and Taurine (CPT) combination in hepatocellular carcinoma (HCC). Methods Twenty-six HCC patients aged (50–80 years) were recruited for administration of a daily dose of 5 g of curcumin, 50 mg of piperine, and 500 mg of taurine divided into three doses for successive 3 months. The three components (CPT) were prepared in one capsule. Patients were assessed after each month (cycle) for the plasma levels of CD4, CD8, CD25, Interleukins-2 (IL-2), IL-6, IL-12, Interferon-gamma (IFN- γ), Lactate dehydrogenase (LDH), and Vascular endothelial growth factor (VEGF), FOXP3 mRNA, and miRNA 21. Results There was a significant increase in the plasma levels of CD4 and CD8, while a significant decrease in the CD25 level after the second and third cycles compared to the baseline level [P < 0.001 for both]. Also, there was a significant increase in the plasma levels of IL-2, IL-12, and IFN-γ [ P = 0.001, P = 0.006, and P = 0.029; respectively], while there was a significant decrease in IL-6, VEGF-α, LDH, and Alpha-fetoprotein (AFP) after CPT administration compared to the baseline levels [P < 0.001, P < 0.001, P = 0.020, and P = 0.004; respectively]. The expression level of miRNA-21 was significantly decreased after CPT administration compared to the baseline level [5.5±0.88, 4.1±0.78, 3±0.75, and 2.5±0.76; respectively, P<0.001]. Though there was a noticeable decrease in the FOXP3 expression after each cycle, however, it didn’t reach a significant level [5.3±0.8, 4.2±0.76, 3.2±0.67, and 2.5±0.79; respectively, P=0.184]. Conclusion CPT could exhibit a potential immune-stimulating effect in HCC patients. The current trial had been registered at the National Hepatology and Tropical Medicine Research Institute (NHTMRI), with a registration number of NHTMRI-IRB 2-21 on 5th January 2021. |
| first_indexed | 2024-03-09T15:04:34Z |
| format | Article |
| id | doaj.art-0dba683d84cf4ef4a47a4926f5766a4f |
| institution | Directory Open Access Journal |
| issn | 2730-6011 |
| language | English |
| last_indexed | 2024-03-09T15:04:34Z |
| publishDate | 2023-09-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj.art-0dba683d84cf4ef4a47a4926f5766a4f2023-11-26T13:43:31ZengSpringerDiscover Oncology2730-60112023-09-0114111410.1007/s12672-023-00785-1The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot studyRaghda R. kotb0Ahmed M. Afifi1Motawa E. EL-Houseini2Mohamed Ezz-Elarab3Emad B. Basalious4Mervat M. Omran5Mona S. Abdellateif6Department of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityMedical Biochemistry and molecular biology, Cancer Biology Department, National Cancer Institute, Cairo UniversityNational Hepatology and Tropical Medicine Research InstitutePharmaceutics and Industrial Pharmacy department, Faculty of Pharmacy, Cairo UniversityPharmacology Unit, Cancer Biology Department, National Cancer Institute, Cairo UniversityMedical Biochemistry and molecular biology, Cancer Biology Department, National Cancer Institute, Cairo UniversityAbstract Background This is a phase II clinical trial to investigate the immunotherapeutic effect of Curcumin, Piperine, and Taurine (CPT) combination in hepatocellular carcinoma (HCC). Methods Twenty-six HCC patients aged (50–80 years) were recruited for administration of a daily dose of 5 g of curcumin, 50 mg of piperine, and 500 mg of taurine divided into three doses for successive 3 months. The three components (CPT) were prepared in one capsule. Patients were assessed after each month (cycle) for the plasma levels of CD4, CD8, CD25, Interleukins-2 (IL-2), IL-6, IL-12, Interferon-gamma (IFN- γ), Lactate dehydrogenase (LDH), and Vascular endothelial growth factor (VEGF), FOXP3 mRNA, and miRNA 21. Results There was a significant increase in the plasma levels of CD4 and CD8, while a significant decrease in the CD25 level after the second and third cycles compared to the baseline level [P < 0.001 for both]. Also, there was a significant increase in the plasma levels of IL-2, IL-12, and IFN-γ [ P = 0.001, P = 0.006, and P = 0.029; respectively], while there was a significant decrease in IL-6, VEGF-α, LDH, and Alpha-fetoprotein (AFP) after CPT administration compared to the baseline levels [P < 0.001, P < 0.001, P = 0.020, and P = 0.004; respectively]. The expression level of miRNA-21 was significantly decreased after CPT administration compared to the baseline level [5.5±0.88, 4.1±0.78, 3±0.75, and 2.5±0.76; respectively, P<0.001]. Though there was a noticeable decrease in the FOXP3 expression after each cycle, however, it didn’t reach a significant level [5.3±0.8, 4.2±0.76, 3.2±0.67, and 2.5±0.79; respectively, P=0.184]. Conclusion CPT could exhibit a potential immune-stimulating effect in HCC patients. The current trial had been registered at the National Hepatology and Tropical Medicine Research Institute (NHTMRI), with a registration number of NHTMRI-IRB 2-21 on 5th January 2021.https://doi.org/10.1007/s12672-023-00785-1Liver cancerHCCCurcuminImmunomodulationPiperineTaurine |
| spellingShingle | Raghda R. kotb Ahmed M. Afifi Motawa E. EL-Houseini Mohamed Ezz-Elarab Emad B. Basalious Mervat M. Omran Mona S. Abdellateif The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study Discover Oncology Liver cancer HCC Curcumin Immunomodulation Piperine Taurine |
| title | The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study |
| title_full | The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study |
| title_fullStr | The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study |
| title_full_unstemmed | The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study |
| title_short | The potential immuno-stimulating effect of curcumin, piperine, and taurine combination in hepatocellular carcinoma; a pilot study |
| title_sort | potential immuno stimulating effect of curcumin piperine and taurine combination in hepatocellular carcinoma a pilot study |
| topic | Liver cancer HCC Curcumin Immunomodulation Piperine Taurine |
| url | https://doi.org/10.1007/s12672-023-00785-1 |
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