| Summary: | In this study, Gram positive and catalase negative rod-shaped bacterial strains were isolated from Iran"s backyard chicken intestines. After a
preliminary screening for acid and bile tolerance, 14 Lactobacillus reuteri isolates were selected based on susceptibility to the antibiotics and
the absence of beta haemolysis for antimicrobial activity against Salmonella Enteritidis and Salmonella Typhimurium. All 14 isolates were
able to tolerate pH 3 for 3 h and their resistance to 0.3% bile salts was more than 50%. Among these 14 isolates, L. reuteri ABRIG22, L. reuteri
ABRIG3, L. reuteri ABRIG18, L. reuteri ABRIG17, L. reuteri ABRIG8 and L. reuteri ABRIG9 were able to withstand pH 2.3 for 2 h, and L. reuteri ABRIG17
was the only isolate with good survivability in pH 2.3 for 3 h. Five isolates were able to withstand stronger acidic conditions and inhibit
Salmonella Enteritidis and Salmonella Typhimurium by more than 90% with less of their supernatants. The results of this study demonstrated
that isolated L. reuteri from intestines of chickens native to Northern Iran could be introduced as potential antimicrobial probiotic strains to
control S. Enteritidis and S. Typhimurium infection.
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