Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i>
Artificial parthenogenetic induction via thermal stimuli in silkworm is an important technique that has been used in sericultural production. However, the molecular mechanism underlying it remains largely unknown. We have created a fully parthenogenetic line (PL) with more than 85% occurrence and 80...
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MDPI AG
2023-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/2/134 |
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| author | Jine Chen Xin Du Xia Xu Sheng Zhang Lusong Yao Xiuling He Yongqiang Wang |
| author_facet | Jine Chen Xin Du Xia Xu Sheng Zhang Lusong Yao Xiuling He Yongqiang Wang |
| author_sort | Jine Chen |
| collection | DOAJ |
| description | Artificial parthenogenetic induction via thermal stimuli in silkworm is an important technique that has been used in sericultural production. However, the molecular mechanism underlying it remains largely unknown. We have created a fully parthenogenetic line (PL) with more than 85% occurrence and 80% hatching rate via hot water treatment and genetic selection, while the parent amphigenetic line (AL) has less than 30% pigmentation rate and less than 1% hatching rate when undergoing the same treatment. Here, isobaric tags for relative and absolute quantitation (iTRAQ)-based analysis were used to investigate the key proteins and pathways associated with silkworm parthenogenesis. We uncovered the unique proteomic features of unfertilized eggs in PL. In total, 274 increased abundance proteins and 211 decreased abundance proteins were identified relative to AL before thermal induction. Function analysis displayed an increased level of translation and metabolism in PL. After thermal induction, 97 increased abundance proteins and 187 decreased abundance proteins were identified. An increase in stress response-related proteins and decrease in energy metabolism suggested that PL has a more effective response to buffer the thermal stress than AL. Cell cycle-related proteins, including histones, and spindle-related proteins were decreased in PL, indicating an important role of this decrease in the process of ameiotic parthenogenesis. |
| first_indexed | 2024-03-11T08:39:20Z |
| format | Article |
| id | doaj.art-0dbbdd938c264986acb849d2b151c91f |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T08:39:20Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-0dbbdd938c264986acb849d2b151c91f2023-11-16T21:14:18ZengMDPI AGInsects2075-44502023-01-0114213410.3390/insects14020134Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i>Jine Chen0Xin Du1Xia Xu2Sheng Zhang3Lusong Yao4Xiuling He5Yongqiang Wang6Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaProteomics and Metabolomics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USAInstitute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaArtificial parthenogenetic induction via thermal stimuli in silkworm is an important technique that has been used in sericultural production. However, the molecular mechanism underlying it remains largely unknown. We have created a fully parthenogenetic line (PL) with more than 85% occurrence and 80% hatching rate via hot water treatment and genetic selection, while the parent amphigenetic line (AL) has less than 30% pigmentation rate and less than 1% hatching rate when undergoing the same treatment. Here, isobaric tags for relative and absolute quantitation (iTRAQ)-based analysis were used to investigate the key proteins and pathways associated with silkworm parthenogenesis. We uncovered the unique proteomic features of unfertilized eggs in PL. In total, 274 increased abundance proteins and 211 decreased abundance proteins were identified relative to AL before thermal induction. Function analysis displayed an increased level of translation and metabolism in PL. After thermal induction, 97 increased abundance proteins and 187 decreased abundance proteins were identified. An increase in stress response-related proteins and decrease in energy metabolism suggested that PL has a more effective response to buffer the thermal stress than AL. Cell cycle-related proteins, including histones, and spindle-related proteins were decreased in PL, indicating an important role of this decrease in the process of ameiotic parthenogenesis.https://www.mdpi.com/2075-4450/14/2/134<i>Bombyx mori</i>parthenogenesisthermal inductionproteomeiTRAQPRM |
| spellingShingle | Jine Chen Xin Du Xia Xu Sheng Zhang Lusong Yao Xiuling He Yongqiang Wang Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> Insects <i>Bombyx mori</i> parthenogenesis thermal induction proteome iTRAQ PRM |
| title | Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> |
| title_full | Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> |
| title_fullStr | Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> |
| title_full_unstemmed | Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> |
| title_short | Comparative Proteomic Analysis Provides New Insights into the Molecular Basis of Thermal-Induced Parthenogenesis in Silkworm (<i>Bombyx mori)</i> |
| title_sort | comparative proteomic analysis provides new insights into the molecular basis of thermal induced parthenogenesis in silkworm i bombyx mori i |
| topic | <i>Bombyx mori</i> parthenogenesis thermal induction proteome iTRAQ PRM |
| url | https://www.mdpi.com/2075-4450/14/2/134 |
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