Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR
In order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, and chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) of specific genomic regions from five selected species and specificity and amplification efficiency were deter...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/19/3034 |
| _version_ | 1797479290501070848 |
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| author | Chuan He Lan Bai Yifan Chen Wei Jiang Junwei Jia Aihu Pan Beibei Lv Xiao Wu |
| author_facet | Chuan He Lan Bai Yifan Chen Wei Jiang Junwei Jia Aihu Pan Beibei Lv Xiao Wu |
| author_sort | Chuan He |
| collection | DOAJ |
| description | In order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, and chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) of specific genomic regions from five selected species and specificity and amplification efficiency were determined. The mixed DNA template with an equal copy number was used for detecting the accuracy and limit of multiplex PCR. The results showed that the primers and probes of the five selected species had good specificity with the minimum number of detection copies: 0.15 copies/µL beef (<i>Bos taurus</i>), 0.28 copies/μL duck (<i>Anas platyrhynchos</i>), 0.37 copies/μL pork (<i>Sus scrofa</i>), 0.39 copies/μL chicken (<i>Gallus gallus</i>), and 0.41 copies/μL mutton (<i>Ovis aries</i>), respectively. The five sets of primers and probes could quickly judge whether the specified meat components existed in the food commodities. |
| first_indexed | 2024-03-09T21:43:43Z |
| format | Article |
| id | doaj.art-0dbd111124f2411f9a51204a20419c89 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:43:43Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-0dbd111124f2411f9a51204a20419c892023-11-23T20:21:05ZengMDPI AGFoods2304-81582022-09-011119303410.3390/foods11193034Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCRChuan He0Lan Bai1Yifan Chen2Wei Jiang3Junwei Jia4Aihu Pan5Beibei Lv6Xiao Wu7Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaShanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaIn order to seek high profit, businesses mix beef and mutton with cheap meat, such as duck, pork, and chicken. Five pairs of primers were designed for quintuple droplet digital PCR (qddPCR) of specific genomic regions from five selected species and specificity and amplification efficiency were determined. The mixed DNA template with an equal copy number was used for detecting the accuracy and limit of multiplex PCR. The results showed that the primers and probes of the five selected species had good specificity with the minimum number of detection copies: 0.15 copies/µL beef (<i>Bos taurus</i>), 0.28 copies/μL duck (<i>Anas platyrhynchos</i>), 0.37 copies/μL pork (<i>Sus scrofa</i>), 0.39 copies/μL chicken (<i>Gallus gallus</i>), and 0.41 copies/μL mutton (<i>Ovis aries</i>), respectively. The five sets of primers and probes could quickly judge whether the specified meat components existed in the food commodities.https://www.mdpi.com/2304-8158/11/19/3034meat adulterationfood quality controlquantificationnucleic acid detectiondroplet digital PCR |
| spellingShingle | Chuan He Lan Bai Yifan Chen Wei Jiang Junwei Jia Aihu Pan Beibei Lv Xiao Wu Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR Foods meat adulteration food quality control quantification nucleic acid detection droplet digital PCR |
| title | Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR |
| title_full | Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR |
| title_fullStr | Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR |
| title_full_unstemmed | Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR |
| title_short | Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR |
| title_sort | detection and quantification of adulterated beef and mutton products by multiplex droplet digital pcr |
| topic | meat adulteration food quality control quantification nucleic acid detection droplet digital PCR |
| url | https://www.mdpi.com/2304-8158/11/19/3034 |
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