A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition
Objectives Osteosarcoma (OS) is a malignant tumor with frequent occurrence among teenagers. Long non‐coding RNAs (lncRNAs) play pro‐cancer roles in many tumors. The purpose of this study was to figure out the functional role of a novel lncRNA long intergenic non‐protein coding RNA 665 (LINC00665) in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Orthopaedic Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/os.13532 |
| _version_ | 1797954076778954752 |
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| author | Jinyu Bai Xiao Zhang Fengxian Jiang Huajian Shan Xiang Gao Lin Bo Yingzi Zhang |
| author_facet | Jinyu Bai Xiao Zhang Fengxian Jiang Huajian Shan Xiang Gao Lin Bo Yingzi Zhang |
| author_sort | Jinyu Bai |
| collection | DOAJ |
| description | Objectives Osteosarcoma (OS) is a malignant tumor with frequent occurrence among teenagers. Long non‐coding RNAs (lncRNAs) play pro‐cancer roles in many tumors. The purpose of this study was to figure out the functional role of a novel lncRNA long intergenic non‐protein coding RNA 665 (LINC00665) in OS by observing the OS cell behaviors. Methods Quantitative reverse transcription polymerase chain reaction (RT‐qPCR) was used to analyze LINC00665 expression in OS cells. Cell function assays assessed the impacts of LINC00665 on OS cell phenotype. Immunofluorescence and western blot analyzed the function of LINC00665 on epithelial‐mesenchymal transition (EMT) in OS. Moreover, mechanistic assays analyzed the downstream mechanism of LINC00665 in OS cells. Results LINC00665 was significantly up‐regulated in OS cells. LINC00665 silence facilitated OS cell proliferation, migration, invasion, and EMT while inhibiting cell apoptosis. Mechanically, LINC00665 acted as a competing endogenous RNA (ceRNA) to sponge miR‐1249‐5p and thereby modulated Wnt family member 2B (WNT2B) to activate Wnt pathway. Wnt pathway activated LINC00665 expression transcriptionally. Conclusions Our study uncovered the cancer‐promoting role of LINC00665 in OS, and the feedback loop of LINC00665/miR‐1249‐5p/WNT2B/Wnt might be a potential target for OS treatment. |
| first_indexed | 2024-04-10T23:11:51Z |
| format | Article |
| id | doaj.art-0dbdffb842c949418f4e9e17acbb93a2 |
| institution | Directory Open Access Journal |
| issn | 1757-7853 1757-7861 |
| language | English |
| last_indexed | 2024-04-10T23:11:51Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Orthopaedic Surgery |
| spelling | doaj.art-0dbdffb842c949418f4e9e17acbb93a22023-01-13T04:29:07ZengWileyOrthopaedic Surgery1757-78531757-78612023-01-0115128630010.1111/os.13532A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal TransitionJinyu Bai0Xiao Zhang1Fengxian Jiang2Huajian Shan3Xiang Gao4Lin Bo5Yingzi Zhang6Department of Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Traditional Chinese Medicine Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Rheumatology the Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Orthopaedics the Second Affiliated Hospital of Soochow University Suzhou ChinaObjectives Osteosarcoma (OS) is a malignant tumor with frequent occurrence among teenagers. Long non‐coding RNAs (lncRNAs) play pro‐cancer roles in many tumors. The purpose of this study was to figure out the functional role of a novel lncRNA long intergenic non‐protein coding RNA 665 (LINC00665) in OS by observing the OS cell behaviors. Methods Quantitative reverse transcription polymerase chain reaction (RT‐qPCR) was used to analyze LINC00665 expression in OS cells. Cell function assays assessed the impacts of LINC00665 on OS cell phenotype. Immunofluorescence and western blot analyzed the function of LINC00665 on epithelial‐mesenchymal transition (EMT) in OS. Moreover, mechanistic assays analyzed the downstream mechanism of LINC00665 in OS cells. Results LINC00665 was significantly up‐regulated in OS cells. LINC00665 silence facilitated OS cell proliferation, migration, invasion, and EMT while inhibiting cell apoptosis. Mechanically, LINC00665 acted as a competing endogenous RNA (ceRNA) to sponge miR‐1249‐5p and thereby modulated Wnt family member 2B (WNT2B) to activate Wnt pathway. Wnt pathway activated LINC00665 expression transcriptionally. Conclusions Our study uncovered the cancer‐promoting role of LINC00665 in OS, and the feedback loop of LINC00665/miR‐1249‐5p/WNT2B/Wnt might be a potential target for OS treatment.https://doi.org/10.1111/os.13532LINC00665miR‐1249‐5pOsteosarcomaWNT2B |
| spellingShingle | Jinyu Bai Xiao Zhang Fengxian Jiang Huajian Shan Xiang Gao Lin Bo Yingzi Zhang A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition Orthopaedic Surgery LINC00665 miR‐1249‐5p Osteosarcoma WNT2B |
| title | A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition |
| title_full | A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition |
| title_fullStr | A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition |
| title_full_unstemmed | A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition |
| title_short | A Feedback Loop of LINC00665 and the Wnt Signaling Pathway Expedites Osteosarcoma Cell Proliferation, Invasion, and Epithelial‐Mesenchymal Transition |
| title_sort | feedback loop of linc00665 and the wnt signaling pathway expedites osteosarcoma cell proliferation invasion and epithelial mesenchymal transition |
| topic | LINC00665 miR‐1249‐5p Osteosarcoma WNT2B |
| url | https://doi.org/10.1111/os.13532 |
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