Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing
In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4340 |
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| author | Orlando Santoro Miryam Chiara Malacarne Francesco Sarcone Luca Scapinello Stefania Pragliola Enrico Caruso Viviana Teresa Orlandi Lorella Izzo |
| author_facet | Orlando Santoro Miryam Chiara Malacarne Francesco Sarcone Luca Scapinello Stefania Pragliola Enrico Caruso Viviana Teresa Orlandi Lorella Izzo |
| author_sort | Orlando Santoro |
| collection | DOAJ |
| description | In this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in the backbone. The P(MMA-<i>ran</i>-DMAEMA) copolymers have inherently bactericidal activity, due to the amino groups of DMAEMA and to the quaternized nitrogens bounded to BODIPY. Systems consisting of filter paper discs coated with copolymers conjugated to BODIPY were tested on two model microorganisms, <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>). On solid medium, irradiation with green light induced an antimicrobial effect, visible as a clear inhibition area around the coated disks. The system based on the copolymer with 43% DMAEMA and circa 0.70 wt/wt% of BODIPY was the most efficient in both bacterial species, and a selectivity for the Gram-positive model was observed, independently of the conjugated BODIPY. A residual antimicrobial activity was also observed after dark incubation, attributed to the inherently bactericidal properties of copolymers. |
| first_indexed | 2024-03-11T07:23:11Z |
| format | Article |
| id | doaj.art-0dc18ab427314aff915244108decc668 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:23:11Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0dc18ab427314aff915244108decc6682023-11-17T07:47:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245434010.3390/ijms24054340Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound DressingOrlando Santoro0Miryam Chiara Malacarne1Francesco Sarcone2Luca Scapinello3Stefania Pragliola4Enrico Caruso5Viviana Teresa Orlandi6Lorella Izzo7Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Chimica e Biologia, Università degli Studi di Salerno, Via Giovanni Paolo II, 128, 85085 Fisciano, SA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyDipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant, 3, 21100 Varese, VA, ItalyIn this work, two compounds belonging to the BODIPY family, and previously investigated for their photosensitizing properties, have been bound to the amino-pendant groups of three random copolymers, with different amounts of methyl methacrylate (MMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) in the backbone. The P(MMA-<i>ran</i>-DMAEMA) copolymers have inherently bactericidal activity, due to the amino groups of DMAEMA and to the quaternized nitrogens bounded to BODIPY. Systems consisting of filter paper discs coated with copolymers conjugated to BODIPY were tested on two model microorganisms, <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>). On solid medium, irradiation with green light induced an antimicrobial effect, visible as a clear inhibition area around the coated disks. The system based on the copolymer with 43% DMAEMA and circa 0.70 wt/wt% of BODIPY was the most efficient in both bacterial species, and a selectivity for the Gram-positive model was observed, independently of the conjugated BODIPY. A residual antimicrobial activity was also observed after dark incubation, attributed to the inherently bactericidal properties of copolymers.https://www.mdpi.com/1422-0067/24/5/4340antimicrobial materialsphotosensitizersBODIPYantimicrobial photodynamic therapy (aPDT)photoinactivation |
| spellingShingle | Orlando Santoro Miryam Chiara Malacarne Francesco Sarcone Luca Scapinello Stefania Pragliola Enrico Caruso Viviana Teresa Orlandi Lorella Izzo Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing International Journal of Molecular Sciences antimicrobial materials photosensitizers BODIPY antimicrobial photodynamic therapy (aPDT) photoinactivation |
| title | Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing |
| title_full | Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing |
| title_fullStr | Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing |
| title_full_unstemmed | Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing |
| title_short | Inherently Antimicrobial P(MMA-<i>ran</i>-DMAEMA) Copolymers Sensitive to Photodynamic Therapy: A Double Bactericidal Effect for Active Wound Dressing |
| title_sort | inherently antimicrobial p mma i ran i dmaema copolymers sensitive to photodynamic therapy a double bactericidal effect for active wound dressing |
| topic | antimicrobial materials photosensitizers BODIPY antimicrobial photodynamic therapy (aPDT) photoinactivation |
| url | https://www.mdpi.com/1422-0067/24/5/4340 |
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