Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects
Graphene oxide (GO) with their interesting properties including thermal and electrical conductivity and antibacterial characteristics have many promising applications in medicine. The prevalence of resistant bacteria is considered a public health problem worldwide, herein, GO has been used as a broa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02995/full |
| _version_ | 1818364056304615424 |
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| author | María Paulina Romero María Paulina Romero Valeria Spolon Marangoni Clara Gonçalves de Faria Ilaiali Souza Leite Cecília de Carvalho Castro e Silva Camila Marchetti Maroneze Marcelo A. Pereira-da-Silva Vanderlei Salvador Bagnato Natalia Mayumi Inada |
| author_facet | María Paulina Romero María Paulina Romero Valeria Spolon Marangoni Clara Gonçalves de Faria Ilaiali Souza Leite Cecília de Carvalho Castro e Silva Camila Marchetti Maroneze Marcelo A. Pereira-da-Silva Vanderlei Salvador Bagnato Natalia Mayumi Inada |
| author_sort | María Paulina Romero |
| collection | DOAJ |
| description | Graphene oxide (GO) with their interesting properties including thermal and electrical conductivity and antibacterial characteristics have many promising applications in medicine. The prevalence of resistant bacteria is considered a public health problem worldwide, herein, GO has been used as a broad spectrum selective antibacterial agent based on the photothermal therapy (PTT)/photodynamic therapy (PDT) effect. The preparation, characterization, determination of photophysical properties of two different sizes of GO is described. In vitro light dose and concentration-dependent studies were performed using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria based on the PTT/PDT effect used ultra-low doses (65 mW cm–2) of 630 nm light, to achieve efficient bacterial decontamination. The results show that GO and nanographene oxide (nGO) can sensitize the formation of 1O2 and allow a temperature rise of 55°C to 60°C together nGO and GO to exert combined PTT/PDT effect in the disinfection of gram-positive S. aureus and gram-negative E. coli bacteria. A complete elimination of S. aureus and E. coli bacteria based on GO and nGO is obtained by using a dose of 43–47 J cm–2 for high concentration used in this study, and a dose of around 70 J cm–2 for low dose of GO and nGO. The presence of high concentrations of GO allows the bacterial population of S. aureus and E. coli to be more sensitive to the use of PDT/PTT and the efficiency of S. aureus and E. coli bacteria disinfection in the presence of GO is similar to that of nGO. In human neonatal dermal fibroblast, HDFs, no significant alteration to cell viability was promoted by GO, but in nGO is observed a mild damage in the HDFs cells independent of nGO concentration and light exposure. The unique properties of GO and nGO may be useful for the clinical treatment of disinfection of broad-spectrum antimicrobials. The antibacterial results of PTT and PDT using GO in gram-positive and gram-negative bacteria, using low dose light, allow us to conclude that GO and nGO can be used in dermatologic infections, since the effect on human dermal fibroblasts of this treatment is low compared to the antibacterial effect. |
| first_indexed | 2024-12-13T21:58:18Z |
| format | Article |
| id | doaj.art-0dc3727358fb44bd8aa6638ac6564381 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T21:58:18Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0dc3727358fb44bd8aa6638ac65643812022-12-21T23:30:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.02995497869Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal EffectsMaría Paulina Romero0María Paulina Romero1Valeria Spolon Marangoni2Clara Gonçalves de Faria3Ilaiali Souza Leite4Cecília de Carvalho Castro e Silva5Camila Marchetti Maroneze6Marcelo A. Pereira-da-Silva7Vanderlei Salvador Bagnato8Natalia Mayumi Inada9São Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilDepartment of Materials, Escuela Politécnica Nacional, Quito, EcuadorMackGraphe, Mackenzie Presbyterian University, São Paulo, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilMackGraphe, Mackenzie Presbyterian University, São Paulo, BrazilMackGraphe, Mackenzie Presbyterian University, São Paulo, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilSão Carlos Institute of Physics, University of São Paulo, São Carlos, BrazilGraphene oxide (GO) with their interesting properties including thermal and electrical conductivity and antibacterial characteristics have many promising applications in medicine. The prevalence of resistant bacteria is considered a public health problem worldwide, herein, GO has been used as a broad spectrum selective antibacterial agent based on the photothermal therapy (PTT)/photodynamic therapy (PDT) effect. The preparation, characterization, determination of photophysical properties of two different sizes of GO is described. In vitro light dose and concentration-dependent studies were performed using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria based on the PTT/PDT effect used ultra-low doses (65 mW cm–2) of 630 nm light, to achieve efficient bacterial decontamination. The results show that GO and nanographene oxide (nGO) can sensitize the formation of 1O2 and allow a temperature rise of 55°C to 60°C together nGO and GO to exert combined PTT/PDT effect in the disinfection of gram-positive S. aureus and gram-negative E. coli bacteria. A complete elimination of S. aureus and E. coli bacteria based on GO and nGO is obtained by using a dose of 43–47 J cm–2 for high concentration used in this study, and a dose of around 70 J cm–2 for low dose of GO and nGO. The presence of high concentrations of GO allows the bacterial population of S. aureus and E. coli to be more sensitive to the use of PDT/PTT and the efficiency of S. aureus and E. coli bacteria disinfection in the presence of GO is similar to that of nGO. In human neonatal dermal fibroblast, HDFs, no significant alteration to cell viability was promoted by GO, but in nGO is observed a mild damage in the HDFs cells independent of nGO concentration and light exposure. The unique properties of GO and nGO may be useful for the clinical treatment of disinfection of broad-spectrum antimicrobials. The antibacterial results of PTT and PDT using GO in gram-positive and gram-negative bacteria, using low dose light, allow us to conclude that GO and nGO can be used in dermatologic infections, since the effect on human dermal fibroblasts of this treatment is low compared to the antibacterial effect.https://www.frontiersin.org/article/10.3389/fmicb.2019.02995/fullphotodynamic-photothermic effectsinglet oxygenbroad-spectrum antibacterialgraphene oxidenano graphene oxidebacterial decontamination |
| spellingShingle | María Paulina Romero María Paulina Romero Valeria Spolon Marangoni Clara Gonçalves de Faria Ilaiali Souza Leite Cecília de Carvalho Castro e Silva Camila Marchetti Maroneze Marcelo A. Pereira-da-Silva Vanderlei Salvador Bagnato Natalia Mayumi Inada Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects Frontiers in Microbiology photodynamic-photothermic effect singlet oxygen broad-spectrum antibacterial graphene oxide nano graphene oxide bacterial decontamination |
| title | Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects |
| title_full | Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects |
| title_fullStr | Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects |
| title_full_unstemmed | Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects |
| title_short | Graphene Oxide Mediated Broad-Spectrum Antibacterial Based on Bimodal Action of Photodynamic and Photothermal Effects |
| title_sort | graphene oxide mediated broad spectrum antibacterial based on bimodal action of photodynamic and photothermal effects |
| topic | photodynamic-photothermic effect singlet oxygen broad-spectrum antibacterial graphene oxide nano graphene oxide bacterial decontamination |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02995/full |
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