The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa
In order to contribute to the development of semen processing procedures in camelids, the aims of the present study were to evaluate (i) the effect of 35% seminal plasma incubation on dromedary camel epididymal sperm motility and kinematic parameters, (ii) the effects of centrifugation, with cushion...
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MDPI AG
2023-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/17/2685 |
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| author | Davide Monaco Giovanni Michele Lacalandra Zeeshan Ansar Paolo Trerotoli Brendan Patrick Mulligan Taher Kamal Osman |
| author_facet | Davide Monaco Giovanni Michele Lacalandra Zeeshan Ansar Paolo Trerotoli Brendan Patrick Mulligan Taher Kamal Osman |
| author_sort | Davide Monaco |
| collection | DOAJ |
| description | In order to contribute to the development of semen processing procedures in camelids, the aims of the present study were to evaluate (i) the effect of 35% seminal plasma incubation on dromedary camel epididymal sperm motility and kinematic parameters, (ii) the effects of centrifugation, with cushion fluid and enzymatic reduction of viscosity (Papain + E64) during ejaculate processing, on the motility and kinematic parameters of dromedary camel ejaculates. The incubation with seminal plasma significantly reduced the percentage of progressively motile spermatozoa as well as the proportion of medium progressive spermatozoa whilst increasing the percentage of non-progressive spermatozoa. The centrifugation procedure improved the sperms’ kinematic parameters, and the highest values were observed for samples centrifugated with cushion fluid. The samples treated with Papain + E64 showed a significant increase in both total and medium progressive spermatozoa, along with a reduction of non-progressive spermatozoa (<i>p</i> < 0.05). The results of this investigation show that a simple, cheap, and effective procedure, such as cushioned centrifugation, could improve the motility patterns of dromedary camel spermatozoa; in combination with enzymatic reduction of viscosity, this method leads to the best results in terms of recovery rates and sperms’ kinematic parameters. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:28:19Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-0dc3860c9e55449e96d7e9ba02ff419d2023-11-19T07:46:01ZengMDPI AGAnimals2076-26152023-08-011317268510.3390/ani13172685The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull SpermatozoaDavide Monaco0Giovanni Michele Lacalandra1Zeeshan Ansar2Paolo Trerotoli3Brendan Patrick Mulligan4Taher Kamal Osman5Department of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano (BA), ItalyDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano (BA), ItalyDepartment of Advanced Biotechnology and Research, Salam Veterinary Group, Buraydah 51911, Saudi ArabiaInterdisciplinary Department of Medicine, University of Bari Aldo Moro, 70124 Bari, ItalyCamel Reproduction Centre, Dubai P.O. Box 79914, United Arab EmiratesDepartment of Advanced Biotechnology and Research, Salam Veterinary Group, Buraydah 51911, Saudi ArabiaIn order to contribute to the development of semen processing procedures in camelids, the aims of the present study were to evaluate (i) the effect of 35% seminal plasma incubation on dromedary camel epididymal sperm motility and kinematic parameters, (ii) the effects of centrifugation, with cushion fluid and enzymatic reduction of viscosity (Papain + E64) during ejaculate processing, on the motility and kinematic parameters of dromedary camel ejaculates. The incubation with seminal plasma significantly reduced the percentage of progressively motile spermatozoa as well as the proportion of medium progressive spermatozoa whilst increasing the percentage of non-progressive spermatozoa. The centrifugation procedure improved the sperms’ kinematic parameters, and the highest values were observed for samples centrifugated with cushion fluid. The samples treated with Papain + E64 showed a significant increase in both total and medium progressive spermatozoa, along with a reduction of non-progressive spermatozoa (<i>p</i> < 0.05). The results of this investigation show that a simple, cheap, and effective procedure, such as cushioned centrifugation, could improve the motility patterns of dromedary camel spermatozoa; in combination with enzymatic reduction of viscosity, this method leads to the best results in terms of recovery rates and sperms’ kinematic parameters.https://www.mdpi.com/2076-2615/13/17/2685epididymal spermatozoaseminal plasmaejaculatesdilutionrecovery ratePapain |
| spellingShingle | Davide Monaco Giovanni Michele Lacalandra Zeeshan Ansar Paolo Trerotoli Brendan Patrick Mulligan Taher Kamal Osman The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa Animals epididymal spermatozoa seminal plasma ejaculates dilution recovery rate Papain |
| title | The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa |
| title_full | The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa |
| title_fullStr | The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa |
| title_full_unstemmed | The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa |
| title_short | The Effect of Cushioned Centrifugation, with and without Enzymatic Reduction of Viscosity, on the Motility Pattern and Kinematic Parameters of Dromedary Camel Bull Spermatozoa |
| title_sort | effect of cushioned centrifugation with and without enzymatic reduction of viscosity on the motility pattern and kinematic parameters of dromedary camel bull spermatozoa |
| topic | epididymal spermatozoa seminal plasma ejaculates dilution recovery rate Papain |
| url | https://www.mdpi.com/2076-2615/13/17/2685 |
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