Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis
Objective: It has recently been shown that the melanoma antigen gene (MAGE) family is expressed in various tumor cell lines but silent in normal tissues, except germ cell lines. Mageb4, a member of the MAGE family, is highly expressed in the testis and homologous in humans and mice. Whole-exome sequ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2020-01-01
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| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2020;volume=4;issue=3;spage=129;epage=136;aulast=Gao |
| _version_ | 1818470990456291328 |
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| author | Sheng Gao Da-Min Yun Li-Wei Zhou Yun-Hao Wu Deng-Feng Lin Xiao-Long Wu Fei Sun |
| author_facet | Sheng Gao Da-Min Yun Li-Wei Zhou Yun-Hao Wu Deng-Feng Lin Xiao-Long Wu Fei Sun |
| author_sort | Sheng Gao |
| collection | DOAJ |
| description | Objective: It has recently been shown that the melanoma antigen gene (MAGE) family is expressed in various tumor cell lines but silent in normal tissues, except germ cell lines. Mageb4, a member of the MAGE family, is highly expressed in the testis and homologous in humans and mice. Whole-exome sequencing studies have identified Mageb4 as a possible X-linked cause of inherited male infertility. However, the function of Mageb4 protein remains largely unknown.
Methods: Using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 technology, we generated a Mageb4 knockout mouse model (Mageb4−/Y) to explore the role of this gene in spermatogenesis.
Results: First, immunostaining of testicular cells showed that Mageb4 is localized in the cytoplasm of spermatogonia. Second, Mageb4−/Y male mice displayed significant increases in apoptosis. However, Mageb4−/Y male mice showed normal fertility, including normal sperm concentration, sperm motility, and testicular and epididymal histology.
Conclusions: These findings suggest that, despite testis-exclusive expression, Mageb4 is dispensable for mouse spermatogenesis. Future research should focus on the role of this gene in apoptosis, aiming to provide clinical guidance regarding male infertility. |
| first_indexed | 2024-04-13T21:45:02Z |
| format | Article |
| id | doaj.art-0dc3ba37a0724d3d9655bdc6f8eb44ec |
| institution | Directory Open Access Journal |
| issn | 2096-2924 2589-8728 |
| language | English |
| last_indexed | 2024-04-13T21:45:02Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-0dc3ba37a0724d3d9655bdc6f8eb44ec2022-12-22T02:28:35ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242589-87282020-01-014312913610.4103/2096-2924.296546Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesisSheng GaoDa-Min YunLi-Wei ZhouYun-Hao WuDeng-Feng LinXiao-Long WuFei SunObjective: It has recently been shown that the melanoma antigen gene (MAGE) family is expressed in various tumor cell lines but silent in normal tissues, except germ cell lines. Mageb4, a member of the MAGE family, is highly expressed in the testis and homologous in humans and mice. Whole-exome sequencing studies have identified Mageb4 as a possible X-linked cause of inherited male infertility. However, the function of Mageb4 protein remains largely unknown. Methods: Using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 technology, we generated a Mageb4 knockout mouse model (Mageb4−/Y) to explore the role of this gene in spermatogenesis. Results: First, immunostaining of testicular cells showed that Mageb4 is localized in the cytoplasm of spermatogonia. Second, Mageb4−/Y male mice displayed significant increases in apoptosis. However, Mageb4−/Y male mice showed normal fertility, including normal sperm concentration, sperm motility, and testicular and epididymal histology. Conclusions: These findings suggest that, despite testis-exclusive expression, Mageb4 is dispensable for mouse spermatogenesis. Future research should focus on the role of this gene in apoptosis, aiming to provide clinical guidance regarding male infertility.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2020;volume=4;issue=3;spage=129;epage=136;aulast=Gaoclustered regularly interspaced palindromic repeats/crispr-associated protein 9; mageb4; male infertility; spermatogenesis |
| spellingShingle | Sheng Gao Da-Min Yun Li-Wei Zhou Yun-Hao Wu Deng-Feng Lin Xiao-Long Wu Fei Sun Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis Reproductive and Developmental Medicine clustered regularly interspaced palindromic repeats/crispr-associated protein 9; mageb4; male infertility; spermatogenesis |
| title | Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis |
| title_full | Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis |
| title_fullStr | Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis |
| title_full_unstemmed | Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis |
| title_short | Mageb4, a testis-specific gene, is dispensable for mouse spermatogenesis |
| title_sort | mageb4 a testis specific gene is dispensable for mouse spermatogenesis |
| topic | clustered regularly interspaced palindromic repeats/crispr-associated protein 9; mageb4; male infertility; spermatogenesis |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2020;volume=4;issue=3;spage=129;epage=136;aulast=Gao |
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