3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties
Extracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423001266 |
| _version_ | 1797796369542414336 |
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| author | Zahra Bashiri Motahareh Rajabi Fomeshi Hatef Ghasemi Hamidabadi Davod Jafari Sanaz Alizadeh Maryam Nazm Bojnordi Gorka Orive Alireza Dolatshahi-Pirouz Maria Zahiri Rui L Reis Subhas C Kundu Mazaher Gholipourmalekabadi |
| author_facet | Zahra Bashiri Motahareh Rajabi Fomeshi Hatef Ghasemi Hamidabadi Davod Jafari Sanaz Alizadeh Maryam Nazm Bojnordi Gorka Orive Alireza Dolatshahi-Pirouz Maria Zahiri Rui L Reis Subhas C Kundu Mazaher Gholipourmalekabadi |
| author_sort | Zahra Bashiri |
| collection | DOAJ |
| description | Extracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds. In this study, we use different concentrations (1.5%, 3% and 5%w/v) of ECM derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin. The printed hydrogels' morphology, physical structure, mechanical behavior, biocompatibility, and angiogenic property are investigated. The optimized ECM (5%w/v) 3D printed scaffold is applied on full-thickness wounds created in a mouse model. Due to their unique native-like structure, the ECM-based scaffolds provide a non-cytotoxic microenvironment for cell adhesion, infiltration, angiogenesis, and proliferation. In contrast, they do not show any sign of immune response to the host. Notably, the biodegradation, swelling rate, mechanical property, cell adhesion and angiogenesis properties increase with the increase of ECM concentrations in the construct. The ECM 3D printed scaffold implanted into deep wounds increases granulation tissue formation, angiogenesis, and re-epithelialization due to the presence of ECM components in the construct, when compared with printed scaffold with no ECM and no treatment wound. Overall, our findings demonstrate that the 5% ECM 3D scaffold supports the best deep wound regeneration in vivo, produces a skin replacement with a cellular structure comparable to native skin. |
| first_indexed | 2024-03-13T03:32:02Z |
| format | Article |
| id | doaj.art-0dc65cb5f276426daec983b6b3c30e45 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-13T03:32:02Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-0dc65cb5f276426daec983b6b3c30e452023-06-24T05:18:43ZengElsevierMaterials Today Bio2590-00642023-06-01201006663D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing propertiesZahra Bashiri0Motahareh Rajabi Fomeshi1Hatef Ghasemi Hamidabadi2Davod Jafari3Sanaz Alizadeh4Maryam Nazm Bojnordi5Gorka Orive6Alireza Dolatshahi-Pirouz7Maria Zahiri8Rui L Reis9Subhas C Kundu10Mazaher Gholipourmalekabadi11Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Omid Fertility & Infertility Clinic, Hamedan, IranCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, IranCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranNanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), 01006, Vitoria-Gasteiz, Spain; Bioaraba, NanoBioCel Research Group, 01009, Vitoria-Gasteiz, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Institute of Health Carlos III, Av Monforte de Lemos 3-5, 28029, Madrid, Spain; University Institute for Regenerative Medicine and Oral Implantology-UIRMI (UPV/EHU-Fundación Eduardo Anitua), 01007, Vitoria-Gasteiz, Spain; Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore, 169856, SingaporeDepartment of Health Technology, Technical University of Denmark, 2800 Kgs, Lyngby, DenmarkThe Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran; Department of Anatomical Sciences, School of Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran; Corresponding author. The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.3Bs Research Group, I3Bs - Research Institute on Biomaterials, Biodegradable and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, Guimaraes, Portugal3Bs Research Group, I3Bs - Research Institute on Biomaterials, Biodegradable and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, Guimaraes, PortugalCellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; Corresponding author. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran.Extracellular matrix (ECM)-based bioinks has attracted much attention in recent years for 3D printing of native-like tissue constructs. Due to organ unavailability, human placental ECM can be an alternative source for the construction of 3D print composite scaffolds for the treatment of deep wounds. In this study, we use different concentrations (1.5%, 3% and 5%w/v) of ECM derived from the placenta, sodium-alginate and gelatin to prepare a printable bioink biomimicking natural skin. The printed hydrogels' morphology, physical structure, mechanical behavior, biocompatibility, and angiogenic property are investigated. The optimized ECM (5%w/v) 3D printed scaffold is applied on full-thickness wounds created in a mouse model. Due to their unique native-like structure, the ECM-based scaffolds provide a non-cytotoxic microenvironment for cell adhesion, infiltration, angiogenesis, and proliferation. In contrast, they do not show any sign of immune response to the host. Notably, the biodegradation, swelling rate, mechanical property, cell adhesion and angiogenesis properties increase with the increase of ECM concentrations in the construct. The ECM 3D printed scaffold implanted into deep wounds increases granulation tissue formation, angiogenesis, and re-epithelialization due to the presence of ECM components in the construct, when compared with printed scaffold with no ECM and no treatment wound. Overall, our findings demonstrate that the 5% ECM 3D scaffold supports the best deep wound regeneration in vivo, produces a skin replacement with a cellular structure comparable to native skin.http://www.sciencedirect.com/science/article/pii/S2590006423001266PlacentaExtracellular matrixECM bioinkAlginate/gelatin3D printed scaffoldWound healing |
| spellingShingle | Zahra Bashiri Motahareh Rajabi Fomeshi Hatef Ghasemi Hamidabadi Davod Jafari Sanaz Alizadeh Maryam Nazm Bojnordi Gorka Orive Alireza Dolatshahi-Pirouz Maria Zahiri Rui L Reis Subhas C Kundu Mazaher Gholipourmalekabadi 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties Materials Today Bio Placenta Extracellular matrix ECM bioink Alginate/gelatin 3D printed scaffold Wound healing |
| title | 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| title_full | 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| title_fullStr | 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| title_full_unstemmed | 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| title_short | 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| title_sort | 3d printed placental derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties |
| topic | Placenta Extracellular matrix ECM bioink Alginate/gelatin 3D printed scaffold Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423001266 |
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