Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars
To maximize the economic benefits of <i>Hyacinthus orientalis</i> L., the micropropagation and morphogenesis induction of five <i>Hyacinthus</i> cultivars were investigated under four different concentrations of indole acetic acid (IAA) with two cytokinins, benzyl adenine (BA...
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MDPI AG
2023-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/2/176 |
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| author | Hany M. El-Naggar Ashraf M. Shehata Maneea Moubarak Amira R. Osman |
| author_facet | Hany M. El-Naggar Ashraf M. Shehata Maneea Moubarak Amira R. Osman |
| author_sort | Hany M. El-Naggar |
| collection | DOAJ |
| description | To maximize the economic benefits of <i>Hyacinthus orientalis</i> L., the micropropagation and morphogenesis induction of five <i>Hyacinthus</i> cultivars were investigated under four different concentrations of indole acetic acid (IAA) with two cytokinins, benzyl adenine (BA), or kinetin (Kin). Days for morphogenesis initiation and shoot formation in the red cultivars were fewer than in the blue and white cultivars. Blue cultivars showed an increase in fresh weight and chlorophyll content under either BA or Kin, but they showed an increase in shoot height in the BA treatments only. IAA at 1.5 mg/L caused a time reduction in days for morphogenesis induction and shoot formation and enhanced shoot height and fresh weight. Kin had a lesser impact than BA on all parameters at all concentrations. The number of shoots differed significantly among the cultivars. The Murashige and Skoog (MS) medium supplemented with 3.0 mg/L indole-3-butyric acid (IBA) produced the highest percentage of root formation (93.3%), number of roots/plantlet (5.26), and root length (1.10 cm). The Jan Bos cultivar at 3.0 mg/L IBA had the highest mean rooting percentage (100%) and number of roots per plantlet (6.66), while Pink Pearl had the highest root length (1.39 cm). |
| first_indexed | 2024-03-11T08:44:36Z |
| format | Article |
| id | doaj.art-0dc83e3dc889485fadf35c187aaf63ad |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T08:44:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-0dc83e3dc889485fadf35c187aaf63ad2023-11-16T20:49:41ZengMDPI AGHorticulturae2311-75242023-01-019217610.3390/horticulturae9020176Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> CultivarsHany M. El-Naggar0Ashraf M. Shehata1Maneea Moubarak2Amira R. Osman3Department of Floriculture, Faculty of Agriculture, Alexandria University (El-Shatby), Alexandria 21545, EgyptDepartment of Floriculture, Faculty of Agriculture, Alexandria University (El-Shatby), Alexandria 21545, EgyptDepartment of Horticulture, Faculty of Agriculture, Damanhour University, Damanhour 22516, EgyptDepartment of Horticulture, Faculty of Agriculture, Damanhour University, Damanhour 22516, EgyptTo maximize the economic benefits of <i>Hyacinthus orientalis</i> L., the micropropagation and morphogenesis induction of five <i>Hyacinthus</i> cultivars were investigated under four different concentrations of indole acetic acid (IAA) with two cytokinins, benzyl adenine (BA), or kinetin (Kin). Days for morphogenesis initiation and shoot formation in the red cultivars were fewer than in the blue and white cultivars. Blue cultivars showed an increase in fresh weight and chlorophyll content under either BA or Kin, but they showed an increase in shoot height in the BA treatments only. IAA at 1.5 mg/L caused a time reduction in days for morphogenesis induction and shoot formation and enhanced shoot height and fresh weight. Kin had a lesser impact than BA on all parameters at all concentrations. The number of shoots differed significantly among the cultivars. The Murashige and Skoog (MS) medium supplemented with 3.0 mg/L indole-3-butyric acid (IBA) produced the highest percentage of root formation (93.3%), number of roots/plantlet (5.26), and root length (1.10 cm). The Jan Bos cultivar at 3.0 mg/L IBA had the highest mean rooting percentage (100%) and number of roots per plantlet (6.66), while Pink Pearl had the highest root length (1.39 cm).https://www.mdpi.com/2311-7524/9/2/176micropropagationmorphogenesis inductionindole acetic acid (IAA)cytokininsbenzyl adenine (BA) and kinetin (Kin) |
| spellingShingle | Hany M. El-Naggar Ashraf M. Shehata Maneea Moubarak Amira R. Osman Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars Horticulturae micropropagation morphogenesis induction indole acetic acid (IAA) cytokinins benzyl adenine (BA) and kinetin (Kin) |
| title | Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars |
| title_full | Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars |
| title_fullStr | Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars |
| title_full_unstemmed | Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars |
| title_short | Optimization of Morphogenesis and In Vitro Production of Five <i>Hyacinthus orientalis</i> Cultivars |
| title_sort | optimization of morphogenesis and in vitro production of five i hyacinthus orientalis i cultivars |
| topic | micropropagation morphogenesis induction indole acetic acid (IAA) cytokinins benzyl adenine (BA) and kinetin (Kin) |
| url | https://www.mdpi.com/2311-7524/9/2/176 |
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