Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status
The efficacy of two plant growth promoting rhizobacteria (PGPR) viz. Pseudomonas jessenni strain R62 and Pseudomonas synxantha strain R81 was examined for mycorrhization of arbuscular mycorrhizal (AM) fungi (Glomus intraradices), survivability, growth and leaf nutrient status in litchi air-layer sys...
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| Language: | English |
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Elsevier
2021-12-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517421000444 |
| _version_ | 1819320358340132864 |
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| author | Amit Visen Pramodh Narayan Singh Binayak Chakraborty Anand Singh Tejpal Singh Bisht |
| author_facet | Amit Visen Pramodh Narayan Singh Binayak Chakraborty Anand Singh Tejpal Singh Bisht |
| author_sort | Amit Visen |
| collection | DOAJ |
| description | The efficacy of two plant growth promoting rhizobacteria (PGPR) viz. Pseudomonas jessenni strain R62 and Pseudomonas synxantha strain R81 was examined for mycorrhization of arbuscular mycorrhizal (AM) fungi (Glomus intraradices), survivability, growth and leaf nutrient status in litchi air-layer system. Therefore, the litchi air-layers were inoculated with PGPR i.e., Pseudomonad strains and AM fungi alone and with combination during the preparation of air-layers on the mother tree and planting of air-layers in root trainers just after detachment of the fresh air-layers from the mother tree. The scanning electron microscopy of the litchi roots indicated that Pseudomonad strains enhanced the process of mycorrhization of AM fungi and accounted near about 11.5 (tree inoculation) to 14.5 (root trainer inoculation) per cent increase in colonization over the sole inoculation of AM fungi in respective air-layers. No sign of mortality in any air-layered plants was noted in PGPR + AM fungi and sole AM fungi inoculated air-layers up to 18 months of growing. Significantly the highest shoot and root dry weight, and root length were recorded in the air-layers inoculated with both PGPR and AM fungi. This co-inoculation of PGPR with AM fungi was also responsible for the significant enrichment of the primary (N, P and K) and micro (Zn, Cu and Fe) nutrient concentration of the leaves in the litchi air-layers. However, the inoculation of air-layers with these microorganisms failed to produce any significant effects on leaf secondary (Ca, Mg and S) nutrient content. Further, the inoculation treatments had an adverse impact on leaf Mn content. The fresh air-layers inoculated after detachment from the mother tree were performed better for most of the studied parameters than the tree inoculated air-layers. |
| first_indexed | 2024-12-24T11:18:19Z |
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| institution | Directory Open Access Journal |
| issn | 2666-5174 |
| language | English |
| last_indexed | 2024-12-24T11:18:19Z |
| publishDate | 2021-12-01 |
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| series | Current Research in Microbial Sciences |
| spelling | doaj.art-0dcb4579692249edadb9a838e08b64042022-12-21T16:58:18ZengElsevierCurrent Research in Microbial Sciences2666-51742021-12-012100063Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient statusAmit Visen0Pramodh Narayan Singh1Binayak Chakraborty2Anand Singh3Tejpal Singh Bisht4Department of Horticulture, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar- 263 145, Udham Singh Nagar, Uttarakhand, India; Subject Matter Specialist (Horticulture), Krishi Vigyan Kendra, Dr. Rajendra Prasad Central Agricultural University, Sipaya, Gopalganj, 841 501, Bihar, India; Corresponding author.Department of Horticulture, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar- 263 145, Udham Singh Nagar, Uttarakhand, IndiaRegional Research Station (Terai Zone), Uttar Banga Krishi Viswavidyalaya, P.O.: Pundibari- 736 165, Coochbehar, WB, IndiaDepartment of Horticulture, Janta College, C.S.J.M. University, Bakewar, Etawah 206 124, Uttar Pradesh, IndiaDepartment of Horticulture, Chauras Campus, Hemvati Nandan Bahuguna Garhwal University, Srinagar-Garhwal- 246 174, Uttarakhand, IndiaThe efficacy of two plant growth promoting rhizobacteria (PGPR) viz. Pseudomonas jessenni strain R62 and Pseudomonas synxantha strain R81 was examined for mycorrhization of arbuscular mycorrhizal (AM) fungi (Glomus intraradices), survivability, growth and leaf nutrient status in litchi air-layer system. Therefore, the litchi air-layers were inoculated with PGPR i.e., Pseudomonad strains and AM fungi alone and with combination during the preparation of air-layers on the mother tree and planting of air-layers in root trainers just after detachment of the fresh air-layers from the mother tree. The scanning electron microscopy of the litchi roots indicated that Pseudomonad strains enhanced the process of mycorrhization of AM fungi and accounted near about 11.5 (tree inoculation) to 14.5 (root trainer inoculation) per cent increase in colonization over the sole inoculation of AM fungi in respective air-layers. No sign of mortality in any air-layered plants was noted in PGPR + AM fungi and sole AM fungi inoculated air-layers up to 18 months of growing. Significantly the highest shoot and root dry weight, and root length were recorded in the air-layers inoculated with both PGPR and AM fungi. This co-inoculation of PGPR with AM fungi was also responsible for the significant enrichment of the primary (N, P and K) and micro (Zn, Cu and Fe) nutrient concentration of the leaves in the litchi air-layers. However, the inoculation of air-layers with these microorganisms failed to produce any significant effects on leaf secondary (Ca, Mg and S) nutrient content. Further, the inoculation treatments had an adverse impact on leaf Mn content. The fresh air-layers inoculated after detachment from the mother tree were performed better for most of the studied parameters than the tree inoculated air-layers.http://www.sciencedirect.com/science/article/pii/S2666517421000444LitchiPGPRAM fungiAir-layerLeaf nutrient |
| spellingShingle | Amit Visen Pramodh Narayan Singh Binayak Chakraborty Anand Singh Tejpal Singh Bisht Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status Current Research in Microbial Sciences Litchi PGPR AM fungi Air-layer Leaf nutrient |
| title | Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status |
| title_full | Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status |
| title_fullStr | Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status |
| title_full_unstemmed | Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status |
| title_short | Scanning electron microscopy indicates Pseudomonad strains facilitate AMF mycorrhization in litchi (Litchi chinensis Sonn.) air-layers and improving survivability, growth and leaf nutrient status |
| title_sort | scanning electron microscopy indicates pseudomonad strains facilitate amf mycorrhization in litchi litchi chinensis sonn air layers and improving survivability growth and leaf nutrient status |
| topic | Litchi PGPR AM fungi Air-layer Leaf nutrient |
| url | http://www.sciencedirect.com/science/article/pii/S2666517421000444 |
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