CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES
Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages. Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2022-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/4_2022/ostapchenko_4_2022.pdf |
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| author | D.I.Ostapchenko N.P.Vedenicheva L.V.Garmanchuk |
| author_facet | D.I.Ostapchenko N.P.Vedenicheva L.V.Garmanchuk |
| author_sort | D.I.Ostapchenko |
| collection | DOAJ |
| description | Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages.
Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO2 for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours.
Results. When adding PMA, Hericium coralloides (0.017 and 0.035 ?g/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes.
Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action. |
| first_indexed | 2024-03-12T10:27:26Z |
| format | Article |
| id | doaj.art-0dcd60c541ed42e5935270a1290dd970 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T10:27:26Z |
| publishDate | 2022-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-0dcd60c541ed42e5935270a1290dd9702023-09-02T09:33:51ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2022-08-01154272910.15407/biotech15.04.027CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIESD.I.Ostapchenko0N.P.Vedenicheva 1 L.V.Garmanchuk2Taras Shevchenko National University of Kyiv "Institute of Biology and MediKholodny Institute of Botany, National Academy of Sciences of Ukraine, KyivTaras Shevchenko National University of Kyiv "Institute of Biology and MediAim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages. Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO2 for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours. Results. When adding PMA, Hericium coralloides (0.017 and 0.035 ?g/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes. Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action.http://biotechnology.kiev.ua/images/4_2022/ostapchenko_4_2022.pdfwords: hericium coralloidesnbt testoxidative metabolismperitoneal macrophages |
| spellingShingle | D.I.Ostapchenko N.P.Vedenicheva L.V.Garmanchuk CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES Biotechnologia Acta words: hericium coralloides nbt test oxidative metabolism peritoneal macrophages |
| title | CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES |
| title_full | CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES |
| title_fullStr | CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES |
| title_full_unstemmed | CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES |
| title_short | CYTOKININ FRACTION OF THE Hericium coralloides INCREASES OXIDATIVE METABOLISM OF MURINE PERITONEAL MACROPHAGIES |
| title_sort | cytokinin fraction of the hericium coralloides increases oxidative metabolism of murine peritoneal macrophagies |
| topic | words: hericium coralloides nbt test oxidative metabolism peritoneal macrophages |
| url | http://biotechnology.kiev.ua/images/4_2022/ostapchenko_4_2022.pdf |
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