Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/3879 |
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| author | Latiffa Amniai Coline Ple Mathieu Barrier Patricia de Nadai Philippe Marquillies Han Vorng Cécile Chenivesse Anne Tsicopoulos Catherine Duez |
| author_facet | Latiffa Amniai Coline Ple Mathieu Barrier Patricia de Nadai Philippe Marquillies Han Vorng Cécile Chenivesse Anne Tsicopoulos Catherine Duez |
| author_sort | Latiffa Amniai |
| collection | DOAJ |
| description | Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune cells. CCL18 is a constitutive and inducible chemokine involved in allergic diseases. The aim of this study was to evaluate CCL18’s effect on NK cells from allergic and nonallergic donors in terms of both chemotactic and immune effects. Results showed that CCL18 was able to induce migration of NK cells from nonallergic donors in a G-protein-dependent manner, suggesting the involvement of a classical chemokine receptor from the family of seven-transmembrane domain G-protein-coupled receptors. In contrast, NK cells from allergic patients were unresponsive. Similarly, CCL18 was able to induce NK cell cytotoxicity only in nonallergic subjects. Purified NK cells did not express CCR8, one of the receptors described to be involved in CCL18 functions. Finally, the defect in CCL18 response by NK cells from allergic patients was unrelated to a defect in CCL18 binding to NK cells. Overall, our results suggest that some NK cell functions may be defective in allergic diseases. |
| first_indexed | 2024-03-10T12:28:38Z |
| format | Article |
| id | doaj.art-0dcf0bc6ecf042ef9bad6d742ce8908f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:28:38Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0dcf0bc6ecf042ef9bad6d742ce8908f2023-11-21T14:48:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228387910.3390/ijms22083879Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 ChemokineLatiffa Amniai0Coline Ple1Mathieu Barrier2Patricia de Nadai3Philippe Marquillies4Han Vorng5Cécile Chenivesse6Anne Tsicopoulos7Catherine Duez8U1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceU1019–UMR 9017-CIIL-Center for Infection and Immunity of Lille, Institut Pasteur de Lille, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, FranceNatural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune cells. CCL18 is a constitutive and inducible chemokine involved in allergic diseases. The aim of this study was to evaluate CCL18’s effect on NK cells from allergic and nonallergic donors in terms of both chemotactic and immune effects. Results showed that CCL18 was able to induce migration of NK cells from nonallergic donors in a G-protein-dependent manner, suggesting the involvement of a classical chemokine receptor from the family of seven-transmembrane domain G-protein-coupled receptors. In contrast, NK cells from allergic patients were unresponsive. Similarly, CCL18 was able to induce NK cell cytotoxicity only in nonallergic subjects. Purified NK cells did not express CCR8, one of the receptors described to be involved in CCL18 functions. Finally, the defect in CCL18 response by NK cells from allergic patients was unrelated to a defect in CCL18 binding to NK cells. Overall, our results suggest that some NK cell functions may be defective in allergic diseases.https://www.mdpi.com/1422-0067/22/8/3879natural killer cellCCL18migrationcytotoxicityallergy |
| spellingShingle | Latiffa Amniai Coline Ple Mathieu Barrier Patricia de Nadai Philippe Marquillies Han Vorng Cécile Chenivesse Anne Tsicopoulos Catherine Duez Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine International Journal of Molecular Sciences natural killer cell CCL18 migration cytotoxicity allergy |
| title | Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine |
| title_full | Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine |
| title_fullStr | Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine |
| title_full_unstemmed | Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine |
| title_short | Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine |
| title_sort | natural killer cells from allergic donors are defective in their response to ccl18 chemokine |
| topic | natural killer cell CCL18 migration cytotoxicity allergy |
| url | https://www.mdpi.com/1422-0067/22/8/3879 |
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